KEGG   ENZYME: 6.3.5.12Help
Entry
EC 6.3.5.12                 Enzyme                                 

Name
Ni-sirohydrochlorin a,c-diamide synthase;
cfbB (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Carbon-nitrogen ligases with glutamine as amido-N-donor
BRITE hierarchy
Sysname
Ni-sirohydrochlorin:L-glutamine amido-ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + Ni-sirohydrochlorin + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + Ni-sirohydrochlorin a,c-diamide + 2 L-glutamate [RN:R11627]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
Ni-sirohydrochlorin [CPD:C21510];
L-glutamine [CPD:C00064];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
Ni-sirohydrochlorin a,c-diamide [CPD:C21511];
L-glutamate [CPD:C00025]
Comment
The enzyme, studied from the methanogenic archaeon Methanosarcina acetivorans, participates in the biosynthesis of the nickel-containing tetrapyrrole cofactor coenzyme F430, which is required by EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase.
History
EC 6.3.5.12 created 2017
Pathway
Porphyrin and chlorophyll metabolism
Orthology
K22012  
Ni-sirohydrochlorin a,c-diamide synthase
Genes
MAC: 
MA_3626(cbiA2)
MBA: 
MBY: 
MBW: 
MBAR: 
MBAK: 
MMA: 
MMJ: 
MMAC: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MBU: 
Mbur_1039(cbiA_cobB)
MMET: 
MMH: 
MHAZ: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MCON_0502(cobB1)
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MCP_0365(cobB)
MEZ: 
Mtc_0065(cbiA-1)
RCI: 
RCIX2001(cbiA-1)
AVE: 
NCT: 
 » show all
Taxonomy
Reference
1  [PMID:27846569]
  Authors
Zheng K, Ngo PD, Owens VL, Yang XP, Mansoorabadi SO.
  Title
The biosynthetic pathway of coenzyme F430 in methanogenic and methanotrophic archaea.
  Journal
Science. 354 (2016) 339-342.
  Sequence
[mac:MA_3626]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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