KEGG   ENZYME: 6.4.1.7Help
Entry
EC 6.4.1.7                  Enzyme                                 

Name
2-oxoglutarate carboxylase;
oxalosuccinate synthetase;
carboxylating factor for ICDH (incorrect);
CFI;
OGC
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
BRITE hierarchy
Reaction(IUBMB)
ATP + 2-oxoglutarate + HCO3- = ADP + phosphate + oxalosuccinate [RN:R08201]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
2-oxoglutarate [CPD:C00026];
HCO3- [CPD:C00288]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
oxalosuccinate [CPD:C05379]
Comment
A biotin-containing enzyme that requires Mg2+ for activity. It was originally thought [1] that this enzyme was a promoting factor for the carboxylation of 2-oxoglutarate by EC 1.1.1.41, isocitrate dehydrogenase (NAD+), but this has since been disproved [2]. The product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 [2].
History
EC 6.4.1.7 created 2006
Reference
1  [PMID:14731279]
  Authors
Aoshima M, Ishii M, Igarashi Y.
  Title
A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6.
  Journal
Mol. Microbiol. 51 (2004) 791-8.
  Organism
Hydrogenobacter thermophilus
  Sequence
Reference
2  [PMID:17076668]
  Authors
Aoshima M, Igarashi Y.
  Title
A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6.
  Journal
Mol. Microbiol. 62 (2006) 748-59.
  Organism
Hydrogenobacter thermophilus
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
60382-75-4

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