3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase

a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]

R03176;
(other) R01837 R02216 R02499 R02840 R03327 R04163 R04678 R04849 R09920

3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]

This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.

1  [PMID:4226148]

Cheatum SG, Watten JC.

Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.

Biochim. Biophys. Acta. 122 (1966) 1-13.

Bos taurus [GN:bta]

2  [PMID:14220672]

KORITZ SB.

THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.

Biochemistry. 3 (1964) 1098-102.

Rattus norvegicus [GN:rno]

3

Neville, A.M., Orr, J.C. and Engel, L.L.

Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.

Biochem. J. 107 (1968) 20.