| Entry |
|
|---|
| Name |
sepiapterin reductase;
SR |
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
 |
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| Sysname |
7,8-dihydrobiopterin:NADP+ oxidoreductase |
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| Reaction(IUBMB) |
(1) 7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+
[RN:R02975];
(2) tetrahydrobiopterin + 2 NADP+ =
6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH + 2 H+ [RN:R08208] |
|---|
| Reaction(KEGG) |
R02975 R08208;
(other) R01813 R04285
 |
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| Substrate |
7,8-dihydrobiopterin [CPD:C02953];
NADP+ [CPD:C00006];
tetrahydrobiopterin [CPD:C00272] |
|---|
| Product |
sepiapterin [CPD:C00835];
NADPH [CPD:C00005];
H+ [CPD:C00080];
6-pyruvoyl-5,6,7,8-tetrahydropterin [CPD:C03684] |
|---|
| Comment |
This enzyme catalyses the final step in the de novo synthesis of
tetrahydrobiopterin from GTP, with the other enzymes involved being
EC 3.5.4.16 (GTP cyclohydrolase I) and EC 4.2.3.12
(6-pyruvoyltetrahydropterin synthase) [5]. |
|---|
| Pathway |
PATH: ec00790 Folate biosynthesis
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K00072 sepiapterin reductase |
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| Genes |
HSA: 6697(SPR)
PTR: 470403(SPR)
MCC: 705317(SPR)
MMU: 20751(Spr)
RNO: 29270(Spr)
CFA: 483118(SPR)
BTA: 533836(SPR)
ECB: 100059420
MDO: 100010091
OAA: 100091653
GGA: 425255(SPR)
XLA: 380273(spr)
XTR: 100145068(spr)
DRE: 554136(spra)
SPU: 577750
DME: Dmel_CG12116 Dmel_CG12117(Sptr)
DPO: Dpse_GA11411
AGA: AgaP_AGAP008000
AAG: AaeL_AAEL001845
TCA: 662832
BMY: Bm1_29970
NVE: NEMVE_v1g161481
TAD: TRIADDRAFT_58441
DDI: DDB_0191275(SR)
 |
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| Structures |
PDB: 1NAS 1OAA 1SEP 1Z6Z 2BD0 |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:4401291]
Kato S.
Sepiapterin reductase from horse liver: purification and properties
of the enzyme.
Arch. Biochem. Biophys. 146 (1971) 202-14.
Rattus norvegicus [GN:rno], Equus caballus [GN:ecb] |
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Reference
Authors
Title
Journal
Organism
|
2 [PMID:5969298]
Matsubara M, Katoh S, Akino M, Kaufman S.
Sepiapterin reductase.
Biochim. Biophys. Acta. 122 (1966) 202-12.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:7528005]
Werner ER, Schmid M, Werner-Felmayer G, Mayer B, Wachter H.
Synthesis and characterization of 3H-labelled tetrahydrobiopterin.
Biochem. J. 304 ( Pt 1) (1994) 189-93.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:10987137]
Kim YA, Chung HJ, Kim YJ, Choi YK, Hwang YK, Lee SW, Park YS.
Characterization of recombinant Dictyostelium discoideum sepiapterin
reductase expressed in E. coli.
Mol. Cells. 10 (2000) 405-10.
Dictyostelium discoideum [GN:ddi] |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:16510994]
Supangat S, Choi YK, Park YS, Son D, Han CD, Lee KH.
Expression, purification, crystallization and preliminary X-ray
analysis of sepiapterin reductase from Chlorobium tepidum.
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 61 (2005)
202-4.
Chlorobium tepidum |
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Reference
Authors
Title
Journal
Organism
|
6 [PMID:15621425]
Choi YK, Jun SR, Cha EY, Park JS, Park YS.
Sepiapterin reductases from Chlorobium tepidum and Chlorobium
limicola catalyze the synthesis of L-threo-tetrahydrobiopterin from
6-pyruvoyltetrahydropterin.
FEMS. Microbiol. Lett. 242 (2005) 95-9.
Chlorobium tepidum |
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| Other DBs |
ExplorEnz - The Enzyme Database: 1.1.1.153
IUBMB Enzyme Nomenclature: 1.1.1.153
ExPASy - ENZYME nomenclature database: 1.1.1.153
BRENDA, the Enzyme Database: 1.1.1.153
CAS: 9059-48-7 |
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