KEGG   ENZYME: 1.1.1.176Help
Entry
EC 1.1.1.176                Enzyme                                 

Name
12alpha-hydroxysteroid dehydrogenase;
12alpha-hydroxy steroid dehydrogenase;
NAD+-dependent 12alpha-hydroxysteroid dehydrogenase;
NADP+-12alpha-hydroxysteroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
12alpha-hydroxysteroid:NADP+ 12-oxidoreductase
Reaction(IUBMB)
cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate + NADPH + H+ [RN:R02793]
Reaction(KEGG)
Substrate
cholate [CPD:C00695];
NADP+ [CPD:C00006]
Product
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate;
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
Catalyses the oxidation of the 12alpha-hydroxy group of bile acids, both in their free and conjugated form. Also acts on bile alcohols.
History
EC 1.1.1.176 created 1978
Reference
1  [PMID:201289]
  Authors
MacDonald IA, Mahony DE, Jellet JF, Meier CE.
  Title
NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559.
  Journal
Biochim. Biophys. Acta. 489 (1977) 466-76.
Reference
2  [PMID:921266]
  Authors
Mahony DE, Meier CE, Macdonald IA, Holdeman LV.
  Title
Bile salt degradation by nonfermentative clostridia.
  Journal
Appl. Environ. Microbiol. 34 (1977) 419-23.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
61642-40-8

DBGET integrated database retrieval system