KEGG   ENZYME: 1.1.1.270Help
Entry
EC 1.1.1.270                Enzyme                                 

Name
3beta-hydroxysteroid 3-dehydrogenase;
3-keto-steroid reductase;
3-KSR;
HSD17B7 (gene name);
ERG27 (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxysteroid:NADP+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ [RN:R05691]
Reaction(KEGG)
R05691;
(other) R04328 R07495
Show
Substrate
3beta-hydroxysteroid [CPD:C02945];
NADP+ [CPD:C00006]
Product
3-oxosteroid [CPD:C01876];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase [4].
History
EC 1.1.1.270 created 2002, modified 2012
Pathway
Steroid biosynthesis
Metabolic pathways
Orthology
K09827  
3-keto steroid reductase
K13373  
17beta-estradiol 17-dehydrogenase / 3beta-hydroxysteroid 3-dehydrogenase
Genes
HSA: 
51478(HSD17B7)
PTR: 
466046 747814(HSD17B7)
PPS: 
100985156(HSD17B7)
GGO: 
PON: 
100446761(HSD17B7)
NLE: 
100587120(HSD17B7)
MCC: 
MCF: 
102127673(HSD17B7)
CJC: 
100410416(HSD17B7)
MMU: 
15490(Hsd17b7)
RNO: 
29540(Hsd17b7)
CGE: 
100767580(Hsd17b7)
HGL: 
101712707(Hsd17b7)
TUP: 
102473205(HSD17B7)
CFA: 
609364(HSD17B7)
AML: 
UMR: 
103658287(HSD17B7)
FCA: 
101083482(HSD17B7)
PTG: 
102968316(HSD17B7)
BTA: 
505212(HSD17B7)
BOM: 
102266348(HSD17B7)
PHD: 
102316552(HSD17B7)
CHX: 
102179058(HSD17B7)
OAS: 
101110013(HSD17B7)
SSC: 
100155418(HSD17B7)
CFR: 
102512546(HSD17B7)
BACU: 
103014331(HSD17B7)
LVE: 
103084050(HSD17B7)
ECB: 
100059061(HSD17B7)
MYB: 
102251181(HSD17B7)
MYD: 
102767046(HSD17B7)
PALE: 
102892079(HSD17B7)
MDO: 
100016071(HSD17B7)
SHR: 
100926227(HSD17B7)
GGA: 
424367(HSD17B7)
MGP: 
APLA: 
101799136(HSD17B7)
TGU: 
100222823(HSD17B7)
FAB: 
101818521(HSD17B7)
PHI: 
102107312(HSD17B7)
FPG: 
101919410(HSD17B7)
FCH: 
102059217(HSD17B7)
CLV: 
102097738(HSD17B7)
ASN: 
102374189(HSD17B7)
AMJ: 
102565865(HSD17B7)
PSS: 
102461698(HSD17B7)
CMY: 
102944731(HSD17B7)
ACS: 
100556465(hsd17b7)
PBI: 
103063132(HSD17B7)
XLA: 
734717(hsd17b7)
XTR: 
548963(hsd17b7)
DRE: 
100332819 768185(hsd17b7)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102348492(HSD17B7)
CMK: 
103180036(hsd17b7)
BFO: 
CIN: 
SPU: 
HRO: 
LGI: 
TAD: 
AQU: 
SCE: 
YLR100W(ERG27)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0B03680(NCAS0B03680) NCAS_0B05180(NCAS0B05180)
NDI: 
NDAI_0B02550(NDAI0B02550) NDAI_0J01100(NDAI0J01100)
TPF: 
TPHA_0C01040(TPHA0C01040)
TBL: 
TBLA_0D04010(TBLA0D04010)
TDL: 
TDEL_0F04160(TDEL0F04160)
KAF: 
KAFR_0B02860(KAFR0B02860) KAFR_0B05590(KAFR0B05590)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.10750(ERG27) CaO19.3240(ERG27)
CTP: 
COT: 
CDU: 
CD36_26140(ERG27)
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
TMN: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_1904154(AO090003001070)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
TMS: 
DSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT79443(AGABI1DRAFT_79443)
ABV: 
AGABI2DRAFT202140(AGABI2DRAFT_202140)
CPUT: 
SLA: 
MGL: 
PGR: 
MLR: 
WSE: 
 » show all
Taxonomy
Reference
1  [PMID:4387005]
  Authors
Swindell AC, Gaylor JL.
  Title
Investigation of the component reactions of oxidative sterol demethylation. Formation and metabolism of 3-ketosteroid intermediates.
  Journal
J. Biol. Chem. 243 (1968) 5546-55.
Reference
2  [PMID:6946726]
  Authors
Billheimer JT, Alcorn M, Gaylor JL.
  Title
Solubilization and partial purification of a microsomal 3-ketosteroid reductase of cholesterol biosynthesis.
  Journal
Arch. Biochem. Biophys. 211 (1981) 430-8.
Reference
3  [PMID:10535978]
  Authors
Gachotte D, Sen SE, Eckstein J, Barbuch R, Krieger M, Ray BD, Bard M
  Title
Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 96 (1999) 12655-60.
  Sequence
[sce:YLR100W]
Reference
4  [PMID:12829805]
  Authors
Marijanovic Z, Laubner D, Moller G, Gege C, Husen B, Adamski J, Breitling R
  Title
Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis.
  Journal
Mol. Endocrinol. 17 (2003) 1715-25.
  Sequence
[hsa:51478]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
42616-29-5

KEGG   ENZYME: 1.1.1.62Help
Entry
EC 1.1.1.62                 Enzyme                                 

Name
17beta-estradiol 17-dehydrogenase;
20alpha-hydroxysteroid dehydrogenase;
17beta,20alpha-hydroxysteroid dehydrogenase;
17beta-estradiol dehydrogenase;
estradiol dehydrogenase;
estrogen 17-oxidoreductase;
17beta-HSD;
HSD17B7
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
17beta-estradiol:NAD(P)+ 17-oxidoreductase
Reaction(IUBMB)
17beta-estradiol + NAD(P)+ = estrone + NAD(P)H + H+ [RN:R02352 R02353]
Reaction(KEGG)
Substrate
17beta-estradiol;
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
estrone [CPD:C00468];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme oxidizes or reduces the hydroxy/keto group on C17 of estrogens and androgens in mammals and regulates the biological potency of these steroids. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.270, 3beta-hydroxysteroid 3-dehydrogenase [3]. The enzyme also acts on (S)-20-hydroxypregn-4-en-3-one and related compounds, oxidizing the (S)-20-group, but unlike EC 1.1.1.149, 20alpha-hydroxysteroid dehydrogenase, it is Si-specific with respect to NAD(P)+.
History
EC 1.1.1.62 created 1965, modified 1983, modified 1986, modified 2012
Pathway
Steroid hormone biosynthesis
Metabolic pathways
Orthology
K00044  
17beta-estradiol 17-dehydrogenase
K10251  
17beta-estradiol 17-dehydrogenase / very-long-chain 3-oxoacyl-CoA reductase
K13368  
17beta-estradiol 17-dehydrogenase / 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)
K13369  
17beta-estradiol 17-dehydrogenase / all-trans-retinol dehydrogenase (NAD+) / 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)
K13370  
17beta-estradiol 17-dehydrogenase / 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+)
K13373  
17beta-estradiol 17-dehydrogenase / 3beta-hydroxysteroid 3-dehydrogenase
Genes
HSA: 
3292(HSD17B1) 3294(HSD17B2) 51144(HSD17B12) 51478(HSD17B7) 7923(HSD17B8) 8630(HSD17B6)
PTR: 
100614183 454269(HSD17B2) 454689(HSD17B1) 466046 741010(HSD17B12) 746685(HSD17B8) 747814(HSD17B7)
PPS: 
100971612(HSD17B12) 100983435(HSD17B2) 100984876 100985156(HSD17B7) 100993294(HSD17B8) 100994126 100996145(HSD17B6)
GGO: 
101125069 101126579(HSD17B12) 101131453(HSD17B2) 101137638 101139911 101143846(HSD17B8) 101153072(HSD17B6)
PON: 
100439025(HSD17B12) 100446761(HSD17B7) 100456547(HSD17B2) 100456666 100458219(HSD17B6) 100458375 100461538(HSD17B8)
NLE: 
100587120(HSD17B7) 100588757(HSD17B2) 100600308(HSD17B12) 100600794(HSD17B1) 100606923(HSD17B6) 100607728(HSD17B8)
MCC: 
710392(HSD17B1) 713549(HSD17B6) 714282 718119(HSD17B8) 720399
MCF: 
101867269(HSD17B12) 101926544 102119721(HSD17B2) 102122077(HSD17B1) 102127673(HSD17B7) 102134563(HSD17B8)
CJC: 
100387390(HSD17B6) 100390011 100390710(HSD17B2) 100399351(HSD17B12) 100409004(HSD17B1) 100410416(HSD17B7) 100415565(HSD17B8)
MMU: 
14979(H2-Ke6) 15485(Hsd17b1) 15486(Hsd17b2) 15490(Hsd17b7) 27400(Hsd17b6) 56348(Hsd17b12)
RNO: 
100362350 25322(Hsd17b1) 286964(Hsd17b6) 29540(Hsd17b7) 361802(Hsd17b8) 79243(Hsd17b2) 84013(Hsd17b12)
CGE: 
100750866(Hsd17b1) 100751291(Hsd17b12) 100751897(Hsd17b2) 100762147(Hsd17b6) 100766230(Hsd17b8) 100767580(Hsd17b7)
HGL: 
101699287(Hsd17b6) 101712707(Hsd17b7) 101717552(Hsd17b12) 101719685(Hsd17b2) 101720493(Hsd17b1) 101726130(Hsd17b8)
TUP: 
102473205(HSD17B7) 102481564(HSD17B2) 102482283(HSD17B1) 102485005(HSD17B6) 102494154(HSD17B8) 102495234(HSD17B12)
CFA: 
475939(HSD17B12) 481118(HSD17B6) 489690(HSD17B2) 607570(HSD17B1) 607895(HSD17B8) 609364(HSD17B7)
AML: 
UMR: 
103656323(HSD17B6) 103658287(HSD17B7) 103662132(HSD17B2) 103665501(HSD17B8) 103672348(HSD17B12) 103672576(HSD17B1)
FCA: 
101082595(HSD17B12) 101083482(HSD17B7) 101085592(HSD17B2) 101086309(HSD17B8) 101088341(HSD17B6) 101091417(HSD17B1)
PTG: 
102958819(HSD17B2) 102958840(HSD17B1) 102963001(HSD17B12) 102968316(HSD17B7) 102968797(HSD17B8) 102971783(HSD17B6)
BTA: 
353107(HSD17B1) 505212(HSD17B7) 515710(HSD17B2) 532422(HSD17B8) 533086(HSD17B6) 785989(HSD17B1) 789567(HSD17B12)
BOM: 
102266348(HSD17B7) 102274407(HSD17B12) 102278627 102278674(HSD17B2) 102281071(HSD17B1) 102283510(HSD17B6) 102286352(HSD17B8)
PHD: 
102315709 102316552(HSD17B7) 102323155 102327736(HSD17B1) 102332625(HSD17B6) 102336871(HSD17B2) 102340413(HSD17B8) 102344762
CHX: 
102169696 102179058(HSD17B7) 102182621(HSD17B2) 102182909(HSD17B1) 102185066(HSD17B6) 102186887(HSD17B12) 102189913(HSD17B8)
OAS: 
100913161(HSD17B2) 101105400 101110013(HSD17B7) 101110086(HSD17B12) 101110778(HSD17B1) 101112590 101116612(HSD17B8)
SSC: 
100147712(HSD17B1) 100154372(HSD17B8) 100155418(HSD17B7) 100312972(HSD17B12) 100312973(HSD17B2) 100620470(HSD17B6)
CFR: 
102504651(HSD17B8) 102508382 102512546(HSD17B7) 102514993(HSD17B2) 102515430(HSD17B12) 102523786(HSD17B1)
BACU: 
102998439(HSD17B12) 103006419(HSD17B8) 103008169(HSD17B6) 103014202(HSD17B2) 103014331(HSD17B7) 103017434 103019221
LVE: 
103068441(HSD17B12) 103070082(HSD17B1) 103070446(HSD17B2) 103076064(HSD17B6) 103084050(HSD17B7) 103088201(HSD17B8)
ECB: 
100036556(HSD17B1) 100050075(HSD17B12) 100052840(HSD17B6) 100059061(HSD17B7) 100061414(HSD17B8) 100146214(HSD17B2)
MYB: 
102251181(HSD17B7) 102252900(HSD17B8) 102256830(HSD17B1) 102260774(HSD17B2) 102263627(HSD17B12)
MYD: 
102751454(HSD17B2) 102763053(HSD17B1) 102766664(HSD17B8) 102766960(HSD17B12) 102767046(HSD17B7)
PALE: 
102882881(HSD17B2) 102883634(HSD17B6) 102889386(HSD17B12) 102889883(HSD17B8) 102892079(HSD17B7) 102898145(HSD17B1)
MDO: 
100011776(HSD17B1) 100013277(HSD17B12) 100016071(HSD17B7) 100025234(HSD17B8) 100031815
SHR: 
OAA: 
100076931(HSD17B2) 100079057(HSD17B12)
GGA: 
395641(HSD17B1) 415807(HSD17B2) 424367(HSD17B7) 769787(HSD17B12)
MGP: 
APLA: 
101792379(HSD17B2) 101799136(HSD17B7) 101803586(HSD17B12)
TGU: 
100222823(HSD17B7) 100223843(HSD17B12) 100228039(HSD17B2) 100231423 101233632(HSD17B1)
FAB: 
101807632 101810836(HSD17B1) 101810951(HSD17B2) 101818521(HSD17B7)
PHI: 
102100450(HSD17B1) 102103079(HSD17B2) 102106801(HSD17B12) 102107312(HSD17B7)
FPG: 
101915382(HSD17B2) 101917180(HSD17B12) 101919410(HSD17B7)
FCH: 
102048922(HSD17B2) 102049990(HSD17B1) 102056639(HSD17B12) 102059217(HSD17B7)
CLV: 
102085015(HSD17B12) 102095570(HSD17B2) 102097738(HSD17B7)
ASN: 
AMJ: 
102559362(HSD17B12) 102564718(HSD17B1) 102565865(HSD17B7) 102567845 102568488(HSD17B8) 102571332 102572659 102574309(HSD17B2)
PSS: 
CMY: 
102929636(HSD17B8) 102938030(HSD17B12) 102939820 102941631(HSD17B2) 102942502(HSD17B1) 102944543(HSD17B6) 102944731(HSD17B7)
ACS: 
PBI: 
103049647(HSD17B12) 103058540(HSD17B2) 103063132(HSD17B7) 103063622
XLA: 
379747(hsd17b12-b) 398882(rdh16) 495218(hsd17b12-a) 734717(hsd17b7)
XTR: 
100127828 100135184(rdh16) 100489679 100497002(hsd17b2) 548963(hsd17b7) 549425(hsd17b8) 549988(hsd17b12)
DRE: 
100332819 322626(hsd17b12b) 327417(hsd17b12a) 402842(hsd17b1) 449537(hsd17b2) 64815(hsd17b8) 768185(hsd17b7)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102348492(HSD17B7) 102348665 102360275(HSD17B2) 102361445(HSD17B8) 102362094 102364827(HSD17B1)
CMK: 
103174968(hsd17b12) 103180036(hsd17b7) 103191276
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CBR: 
CBG18334(Cbr-dhs-11)
LOA: 
TSP: 
HRO: 
LGI: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
AT1G67730(KCR1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0008s18190g(POPTRDRAFT_565101) POPTR_0010s06220g(POPTRDRAFT_821856) POPTR_0010s06230g(POPTRDRAFT_769035) POPTR_0010s06240g(POPTRDRAFT_565949)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os04t0116600-01(Os04g0116600) Os04t0483500-01(Os04g0483500) Os06t0298700-00(Os06g0298700) Os06t0300200-00(Os06g0300200)
OBR: 
BDI: 
SBI: 
SORBI_01g035200(SORBIDRAFT_01g035200) SORBI_01g047620(SORBIDRAFT_01g047620) SORBI_04g025020(SORBIDRAFT_04g025020) SORBI_06g020580(SORBIDRAFT_06g020580) SORBI_06g020600(SORBIDRAFT_06g020600) SORBI_10g010930(SORBIDRAFT_10g010930) SORBI_10g010960(SORBIDRAFT_10g010960) SORBI_10g010990(SORBIDRAFT_10g010990) SORBI_10g011020(SORBIDRAFT_10g011020)
ZMA: 
542359(gl8)
SITA: 
ATR: 
s00049p00175350(AMTR_s00049p00175350) s00049p00175530(AMTR_s00049p00175530) s00049p00175800(AMTR_s00049p00175800)
SMO: 
PPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YBR159W(IFA38)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0B02360(NCAS0B02360)
NDI: 
NDAI_0C05330(NDAI0C05330)
TPF: 
TPHA_0L01460(TPHA0L01460)
TBL: 
TBLA_0H03430(TBLA0H03430)
TDL: 
TDEL_0B01260(TDEL0B01260)
KAF: 
KAFR_0E03020(KAFR0E03020)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_900014(AO090026000492)
ANG: 
ANI_1_488024(An02g03570)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
TMS: 
PPL: 
DSQ: 
SHS: 
PCO: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT112492(AGABI1DRAFT_112492)
ABV: 
AGABI2DRAFT192460(AGABI2DRAFT_192460)
CPUT: 
SLA: 
UMA: 
PFP: 
MGL: 
PGR: 
MLR: 
WSE: 
EIN: 
NCE: 
MBR: 
DDI: 
DPP: 
DFA: 
EHI: 
EHI_165070(127.t00009)
EDI: 
PCB: 
PVX: 
PCY: 
TGO: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
GLA: 
BCEN: 
BMK: 
 » show all
Taxonomy
Reference
1  [PMID:4314937]
  Authors
Kautsky MP, Hagerman DD.
  Title
17 Beta-estradiol dehydrogenase of ovine ovaries.
  Journal
J. Biol. Chem. 245 (1970) 1978-84.
Reference
2  [PMID:14413943]
  Authors
LANGER LJ, ALEXANDER JA, ENGEL LL.
  Title
Human placental estradiol-17 beta dehydrogenase. II. Kinetics and substrate specificities.
  Journal
J. Biol. Chem. 234 (1959) 2609-14.
Reference
3  [PMID:12829805]
  Authors
Marijanovic Z, Laubner D, Moller G, Gege C, Husen B, Adamski J, Breitling R
  Title
Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis.
  Journal
Mol. Endocrinol. 17 (2003) 1715-25.
  Sequence
[hsa:51478]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9028-61-9

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