| Entry |
|
|---|
| Name |
3alpha(or 20beta)-hydroxysteroid dehydrogenase;
cortisone reductase;
(R)-20-hydroxysteroid dehydrogenase;
dehydrogenase, 20beta-hydroxy steroid;
Delta4-3-ketosteroid hydrogenase;
20beta-hydroxysteroid dehydrogenase;
3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase;
NADH-20beta-hydroxysteroid dehydrogenase;
20beta-HSD |
|---|
| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
 |
|---|
| Sysname |
3alpha(or 20beta)-hydroxysteroid:NAD+ oxidoreductase |
|---|
| Reaction(IUBMB) |
androstan-3alpha,17beta-diol + NAD+ = 17beta-hydroxyandrostan-3-one
+ NADH + H+ [RN:R04330] |
|---|
| Reaction(KEGG) |
R04330;
(other) R04831 R04834 R04844 R04847
 |
|---|
| Substrate |
androstan-3alpha,17beta-diol [CPD:C03852];
NAD+ [CPD:C00003] |
|---|
| Product |
17beta-hydroxyandrostan-3-one [CPD:C03917];
NADH [CPD:C00004];
H+ [CPD:C00080] |
|---|
| Comment |
The 3alpha-hydroxy group or 20beta-hydroxy group of pregnane and
androstane steroids can act as donor. |
|---|
| Pathway |
PATH: ec00140 Steroid hormone biosynthesis
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K00038 3alpha(or 20beta)-hydroxysteroid dehydrogenase |
|---|
| Genes |
PHA: PSHAa0891
BUR: Bcep18194_B1012 Bcep18194_B1510
BCJ: BCAM1582
GME: Gmet_2226 Gmet_2249
RLE: RL1197
OIH: OB0329
LBR: LVIS_0330
MRA: MRA_2018(fabG3-1)
MBO: Mb2025(fabG3)
MBB: BCG_2019(fabG3)
MPA: MAP1739c
MAV: MAV_2509 MAV_5136
MSM: MSMEG_2566 MSMEG_3515
MUL: MUL_1037(fabG3)
NFA: nfa48060
SEN: SACE_4705
STP: Strop_2631
 |
|---|
| Structures |
PDB: 1HDC 1N5D 1NFF 1NFQ 1NFR 2HSD |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:718844]
Edwards CA, Orr JC.
Comparison of the 3alpha-and 20beta-hydroxysteroid dehydrogenase
activities of the cortisone reductase of Streptomyces hydrogenans.
Biochemistry. 17 (1978) 4370-6.
Streptomyces hydrogenans |
|---|
Reference
Authors
Title
Journal
|
2 [PMID:14403761]
HUEBENER HJ, SAHRHOLZ FG
[20 beta-Hydroxysteroid dehydrogenase. 2. Preparation and
crystallization.]
Biochem. Z. 333 (1960) 95-105. |
|---|
Reference
Authors
Title
Journal
|
3 [PMID:14403760]
HUEBENER HJ, SAHRHOLZ FG, SCHMIDT-THOME J, NESEMANN G, JUNK R
[20 beta-Hydroxysteroid dehydrogenase, a new crystalline enzyme.]
Biochim. Biophys. Acta. 35 (1959) 270-2. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:13549484]
LYNN WS Jr, BROWN RH.
The conversion of progesterone to androgens by testes.
J. Biol. Chem. 232 (1958) 1015-30.
Rattus norvegicus [GN:rno], Cavia porcellus |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:6936053]
Strickler RC, Covey DF, Tobias B.
Study of 3 alpha, 20 beta-hydroxysteroid dehydrogenase with an
enzyme-generated affinity alkylator: dual enzyme activity at a
single active site.
Biochemistry. 19 (1980) 4950-4.
Streptomyces hydrogenans |
|---|
Reference
Authors
Title
Journal
Organism
|
6 [PMID:6928375]
Sweet F, Samant BR.
Bifunctional enzyme activity at the same active site: study of 3
alpha and 20 beta activity by affinity alkylation of 3 alpha, 20
beta-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids.
Biochemistry. 19 (1980) 978-86.
Streptomyces hydrogenans |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.1.1.53
IUBMB Enzyme Nomenclature: 1.1.1.53
ExPASy - ENZYME nomenclature database: 1.1.1.53
BRENDA, the Enzyme Database: 1.1.1.53
CAS: 9028-42-6 |
|---|
