| Entry |
|
|---|
| Name |
thiamine oxidase;
thiamin dehydrogenase;
thiamine dehydrogenase;
thiamin:oxygen 5-oxidoreductase |
|---|
| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
 |
|---|
| Sysname |
thiamine:oxygen 5-oxidoreductase |
|---|
| Reaction(IUBMB) |
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2 [RN:R02132] |
|---|
| Reaction(KEGG) |
R02132;
(other) R00033 R00072
 |
|---|
| Substrate |
thiamine [CPD:C00378];
O2 [CPD:C00007];
H2O [CPD:C00001] |
|---|
| Product |
thiamine acetic acid [CPD:C02892];
H2O2 [CPD:C00027] |
|---|
| Cofactor |
FAD [CPD:C00016] |
|---|
| Comment |
A flavoprotein (FAD). The product differs from thiamine in
replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation
proceeds without the release of the intermediate aldehyde from the
enzyme. |
|---|
| Pathway |
PATH: ec00730 Thiamine metabolism |
|---|
Reference
Authors
Title
Journal
|
1 [PMID:8076]
Edmondson DE, Kenney WC, Singer TP.
Structural elucidation and properties of
8alpha-(N1-histidyl)riboflavin: the flavin component of thiamine
dehydrogenase and beta-cyclopiazonate oxidocyclase.
Biochemistry. 15 (1976) 2937-45. |
|---|
Reference
Authors
Title
Journal
|
2 [PMID:2988447]
Gomez-Moreno C, Edmondson DE.
Evidence for an aldehyde intermediate in the catalytic mechanism of
thiamine oxidase.
Arch. Biochem. Biophys. 239 (1985) 46-52. |
|---|
Reference
Authors
Title
Journal
|
3 [PMID:4987737]
Neal RA.
Bacterial metabolism of thiamine. 3. Metabolism of thiamine to
3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic
acid (thiamine acetic acid) by a flavoprotein isolated from a soil
microorganism.
J. Biol. Chem. 245 (1970) 2599-604. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.1.3.23
IUBMB Enzyme Nomenclature: 1.1.3.23
ExPASy - ENZYME nomenclature database: 1.1.3.23
BRENDA, the Enzyme Database: 1.1.3.23
CAS: 96779-44-1 |
|---|
