KEGG   ENZYME: 1.1.3.6Help
Entry
EC 1.1.3.6                  Enzyme                                 
Name
cholesterol oxidase;
cholesterol- O2 oxidoreductase;
3beta-hydroxy steroid oxidoreductase;
3beta-hydroxysteroid:oxygen oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
cholesterol:oxygen oxidoreductase
Reaction(IUBMB)
cholesterol + O2 = cholest-5-en-3-one + H2O2 [RN:R01459]
Reaction(KEGG)
Substrate
cholesterol [CPD:C00187];
O2 [CPD:C00007]
Product
cholest-5-en-3-one;
H2O2 [CPD:C00027]
Comment
Contains flavin adenine dinucleotide (FAD). Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyse the first two steps in the degradation of cholesterol. The enzyme catalyses the oxidation of the 3beta-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Delta5 position to Delta6 position (cf. EC 5.3.3.1, steroid Delta-isomerase).
Pathway
Steroid degradation
Orthology
K03333  
cholesterol oxidase
Genes
PFO: 
PFE: 
PBA: 
CJA: 
CJA_0537(choB)
PRW: 
ACD: 
ACB: 
ABY: 
ABN: 
ABB: 
ACC: 
BDGL_000403(choB)
NHL: 
Nhal_1338 Nhal_1347
HCH: 
HCH_01642
ABO: 
ABO_1259
ADI: 
B5T_02424
TOL: 
TOL_0082 TOL_3041 TOL_3265
GPB: 
HDN1F_24470
CVI: 
CV_2218
RSO: 
RS05823(RSp1167)
RSL: 
RPSI07_mp1199
RSN: 
RSPO_m00590
REH: 
H16_A1655(betA3)
CNC: 
CNE_1c16780(betA2)
BMA: 
BMAA2022
BMV: 
BMASAVP1_1045
BML: 
BMA10229_1332
BMN: 
BMA10247_A2312
BPS: 
BPSS2278
BPM: 
BURPS1710b_A1417
BPL: 
BURPS1106A_A3075
BPD: 
BURPS668_A3202
BPZ: 
BP1026B_II2455
BPQ: 
BPC006_II3022
BTE: 
BTH_II2309
BCT: 
GEM_4737 GEM_4740
BXE: 
Bxe_B1816
BPH: 
Bphy_5113
BPY: 
Bphyt_4847
BUG: 
BC1001_5582
BGE: 
BC1002_3673
BGF: 
BC1003_5676
BPX: 
BUPH_06176
RFR: 
Rfer_0318
LCH: 
Lcho_3191
SLT: 
Slit_1762 Slit_1764
BBA: 
Bd0736(choA) Bd2646(choD)
BBAT: 
Bdt_0702(choA) Bdt_2561(choD)
BMX: 
BMS_1931(choD)
DAL: 
Dalk_4295
ADE: 
Adeh_3081
ACP: 
A2cp1_3274
AFW: 
Anae109_3076
ANK: 
AnaeK_3180
MXA: 
MXAN_1504 MXAN_5165
MFU: 
LILAB_01255 LILAB_27295 LILAB_33825
MSD: 
MYSTI_05670
CCX: 
COCOR_01501(choD) COCOR_02334(choD1) COCOR_04317 COCOR_05798 COCOR_05799
SUR: 
STAUR_5292 STAUR_5929 STAUR_6636
SCL: 
sce0787
HOH: 
Hoch_5037
AVI: 
Avi_3364
PBR: 
PB2503_01622
MTU: 
Rv3409c(choD)
MTV: 
RVBD_3409c
MTC: 
MT3517
MRA: 
MRA_3449(choD)
MTF: 
TBFG_13443
MTB: 
TBMG_03460
MTK: 
TBSG_03482
MTZ: 
TBXG_003435
MTG: 
MRGA327_21035
MTE: 
CCDC5079_3155
MTL: 
CCDC5180_3107
MTO: 
MTCTRI2_3481(choD)
MTD: 
UDA_3409c(choD)
MTN: 
ERDMAN_3730(choD)
MTJ: 
J112_18340
MTUB: 
MT7199_3456
MBO: 
Mb3443c(choD)
MBB: 
BCG_3479c(choD)
MBT: 
JTY_3479(choD)
MBM: 
BCGMEX_3477c(choD)
MBK: 
K60_035470
MAF: 
MAF_34230(choD)
MCE: 
MCAN_34331(choD)
MCQ: 
BN44_80068(choD)
MCV: 
BN43_70062(choD)
MCX: 
BN42_50067(choD)
MCZ: 
BN45_70054(choD)
MLE: 
ML0389(choD)
MLB: 
MLBr_00389(choD)
MAV: 
MAV_4354
MIT: 
OCO_42200
MIR: 
OCQ_43470
MIA: 
OCU_42130
MID: 
MIP_06364
MSM: 
MSMEG_1604
MSG: 
MSMEI_1565(choD)
MSA: 
Mycsm_01128 Mycsm_04230
MUL: 
MUL_0903(choD)
MVA: 
Mvan_1514
MGI: 
Mflv_4906
MSP: 
Mspyr1_43230
MAB: 
MAB_3719c
MMV: 
MYCMA_0661(choD) MYCMA_2043(choD)
MMC: 
Mmcs_1170
MKM: 
Mkms_1187
MJL: 
Mjls_1197
MJD: 
JDM601_0848(choD) JDM601_3221(choD)
MMI: 
MMAR_1140(choD)
MRH: 
MycrhN_0566
MMM: 
W7S_21155
MCB: 
Mycch_1117
MLI: 
MULP_01281(choD)
ASD: 
AS9A_0721 AS9A_2383 AS9A_4029
CJK: 
jk0629(choE)
CUR: 
cur_1318
CUA: 
CU7111_1299(choE)
CRD: 
CRES_0700(choD)
CVA: 
CVAR_1073
NFA: 
nfa26130
NCY: 
NOCYR_0288 NOCYR_1949 NOCYR_2706(choD) NOCYR_2813(choD)
NBR: 
O3I_001555 O3I_020300
RHA: 
RHA1_ro03863 RHA1_ro04305(choD) RHA1_ro06201(choD)
RER: 
RER_04330 RER_10120(choE) RER_19350 RER_28410 RER_31400
ROP: 
ROP_42140 ROP_62610
REQ: 
REQ_13580 REQ_26800 REQ_34910 REQ_43910(choE)
GBR: 
Gbro_1204 Gbro_1728 Gbro_2398 Gbro_2399 Gbro_4774
GPO: 
GPOL_c16080 GPOL_c23320 GPOL_c38540 GPOL_c38690 GPOL_c48920
GOR: 
KTR9_1092 KTR9_1668(choD) KTR9_2317 KTR9_4571
TPR: 
Tpau_0908
SRT: 
Srot_2942
SCO: 
SCO4781(SCD63.13)
SMA: 
SAV_5017(choD)
SGR: 
SGR_2748 SGR_477
SCB: 
SCAB_36081 SCAB_67491
SSX: 
SACTE_4048
SVL: 
Strvi_5148 Strvi_8680
SCT: 
SCAT_3673
SCY: 
SCATT_36640
SFA: 
Sfla_2446 Sfla_5322
SBH: 
SBI_04801(choD) SBI_06081
SHY: 
SHJG_5880
SHO: 
SHJGH_5642
SVE: 
SVEN_0220 SVEN_4463
SDV: 
BN159_3636(choD) BN159_6302(choA)
SALB: 
XNR_3818
STRP: 
F750_1301 F750_4347
KSK: 
KSE_30980(choD)
ART: 
Arth_1255
XCE: 
Xcel_2031
CFL: 
Cfla_3546
CFI: 
Celf_0079 Celf_1299
CGA: 
Celgi_0154
ICA: 
Intca_1023
NCA: 
Noca_3632
KFL: 
Kfla_3907 Kfla_6020 Kfla_6484
TFU: 
Tfu_2332
NDA: 
Ndas_0464
NAL: 
B005_3333(choD)
TCU: 
Tcur_1996 Tcur_4248
SRO: 
Sros_1176 Sros_4246
FRA: 
Francci3_4446
FRE: 
Franean1_0121 Franean1_3356 Franean1_3901
FRI: 
FraEuI1c_0073 FraEuI1c_1561 FraEuI1c_5217
FAL: 
FRAAL6768(choA)
FSY: 
FsymDg_0187 FsymDg_0454 FsymDg_3076
NML: 
Namu_2922
SEN: 
SACE_6417(pteG) SACE_6705(choD)
SVI: 
Svir_04760 Svir_13500 Svir_30650
TBI: 
Tbis_0628
AMD: 
AMED_0775 AMED_7505
AMN: 
RAM_03960 RAM_38570
AMM: 
AMES_0773 AMES_7394
AMI: 
Amir_2425 Amir_3746 Amir_6532
SESP: 
BN6_33640 BN6_78530(choD)
STP: 
Strop_2611 Strop_3834
SAQ: 
Sare_2810 Sare_4224
MAU: 
Micau_2519 Micau_5419
MIL: 
ML5_0512 ML5_5844
VMA: 
VAB18032_00675
AMS: 
AMIS_19390 AMIS_56460 AMIS_67730 AMIS_79290
ASE: 
ACPL_5525(choD) ACPL_7034(choD)
CAI: 
Caci_2199 Caci_5961
SNA: 
Snas_1382 Snas_3535
CWO: 
Cwoe_4226
LIL: 
LA_3998 LA_3999 LA_4009
LIE: 
LIF_A3194 LIF_A3195 LIF_A3201
LIC: 
LIC13194(choB) LIC13195
LBL: 
LBL_0185
LBI: 
LEPBI_I0286(choB)
LBF: 
LBF_0277
TPX: 
Turpa_2392
ABA: 
Acid345_0361
SUS: 
Acid_5726
CMR: 
Cycma_4378
FLI: 
Fleli_0217
MTT: 
Ftrac_1591
WVI: 
Weevi_2091
KDI: 
Krodi_2222
ORH: 
Ornrh_1445
PTQ: 
P700755_001181
OHO: 
Oweho_0738
SYD: 
Syncc9605_0826
NPU: 
Npun_F2280 Npun_R6526
NOP: 
Nos7524_1682 Nos7524_5597
CALT: 
Cal6303_4456
RIV: 
Riv7116_4501
CTHE: 
Chro_2177 Chro_3290 Chro_4542
 » show all
Taxonomy
Reference
1  [PMID:4757363]
  Authors
Richmond W.
  Title
Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.
  Journal
Clin. Chem. 19 (1973) 1350-6.
  Organism
Nocardia sp.
Reference
2  [PMID:13143010]
  Authors
STADTMAN TC, CHERKES A, ANFINSEN CB.
  Title
Studies on the microbiological degradation of cholesterol.
  Journal
J. Biol. Chem. 206 (1954) 511-23.
  Organism
Mycobacterium sp.
Reference
3  [PMID:10822008]
  Authors
MacLachlan J, Wotherspoon AT, Ansell RO, Brooks CJ
  Title
Cholesterol oxidase: sources, physical properties and analytical applications.
  Journal
J. Steroid. Biochem. Mol. Biol. 72 (2000) 169-95.
Reference
4  [PMID:20213543]
  Authors
Vrielink A
  Title
Cholesterol oxidase: structure and function.
  Journal
Subcell. Biochem. 51 (2010) 137-58.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9028-76-6
DBGET integrated database retrieval system