KEGG   ENZYME: 1.11.1.11Help
Entry
EC 1.11.1.11                Enzyme                                 
Name
L-ascorbate peroxidase;
L-ascorbic acid peroxidase;
L-ascorbic acid-specific peroxidase;
ascorbate peroxidase;
ascorbic acid peroxidase
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
BRITE hierarchy
Sysname
L-ascorbate:hydrogen-peroxide oxidoreductase
Reaction(IUBMB)
2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O (overall reaction) [RN:R00644];
(1a) 2 L-ascorbate + H2O2 + 2 H+ = 2 monodehydroascorbate + 2 H2O [RN:R09540];
(1b) 2 monodehydroascorbate = L-ascorbate + L-dehydroascorbate (spontaneous) [RN:R03186]
Reaction(KEGG)
Substrate
L-ascorbate [CPD:C00072];
H2O2 [CPD:C00027];
H+ [CPD:C00080];
monodehydroascorbate [CPD:C01041]
Product
L-ascorbate [CPD:C00072];
L-dehydroascorbate [CPD:C05422];
H2O [CPD:C00001];
monodehydroascorbate [CPD:C01041]
Comment
A heme protein. Oxidizes ascorbate and low molecular weight aromatic substrates. The monodehydroascorbate radical produced is either directly reduced back to ascorbate by EC 1.6.5.4 [monodehydroascorbate reductase (NADH)] or undergoes non-enzymatic disproportionation to ascorbate and dehydroascorbate.
Pathway
Ascorbate and aldarate metabolism
Glutathione metabolism
Orthology
K00434  
L-ascorbate peroxidase
Genes
ATH: 
AT1G07890(APX1) AT1G77490(TAPX) AT3G09640(APX2) AT4G08390(SAPX) AT4G09010(APX4) AT4G32320(APX6) AT4G35000(APX3) AT4G35970(APX5)
ALY: 
GMX: 
MTR: 
CSV: 
RCU: 
POP: 
VVI: 
OSA: 
DOSA: 
Os02t0553200-01(Os02g0553200) Os03t0285700-01(Os03g0285700) Os04t0223300-00(Os04g0223300) Os04t0434800-01(Os04g0434800) Os04t0602100-01(Os04g0602100) Os07t0694700-01(Os07g0694700) Os08t0522400-01(Os08g0522400) Os08t0549100-01(Os08g0549100) Os12t0178100-01(Os12g0178100) Os12t0178200-01(Os12g0178200)
BDI: 
100825789 100826086 100828614 100831503 100836915 100836984 100838031 100838967 100839539
SBI: 
SORBI_01g038760(SORBIDRAFT_01g038760) SORBI_02g044060(SORBIDRAFT_02g044060) SORBI_04g022560(SORBIDRAFT_04g022560) SORBI_06g001970(SORBIDRAFT_06g001970) SORBI_06g017080(SORBIDRAFT_06g017080) SORBI_07g024880(SORBIDRAFT_07g024880) SORBI_07g027300(SORBIDRAFT_07g027300) SORBI_08g004880(SORBIDRAFT_08g004880)
ZMA: 
100193965(cl26634_1a) 100194161 100272974 100282326 100283135(pco111578) 100283822 100285887 100383462 100384480(POD) 542476(apx2)
SMO: 
SELMODRAFT_108251 SELMODRAFT_126894 SELMODRAFT_139337 SELMODRAFT_157825 SELMODRAFT_270176 SELMODRAFT_271672 SELMODRAFT_74674 SELMODRAFT_85469
PPP: 
PHYPADRAFT_119771 PHYPADRAFT_123146 PHYPADRAFT_148437 PHYPADRAFT_177021 PHYPADRAFT_227509 PHYPADRAFT_63071
CRE: 
CHLREDRAFT_165193 CHLREDRAFT_186597(APX1) CHLREDRAFT_205910(APX2)
VCN: 
VOLCADRAFT_105827 VOLCADRAFT_62034(apx2) VOLCADRAFT_82395(apx1)
OLU: 
OSTLU_32890 OSTLU_6186 OSTLU_93205
OTA: 
Ot01g03570 Ot08g00200 Ot09g00500
CME: 
CMM158C
ZTR: 
MYCGRDRAFT_33834 MYCGRDRAFT_77793
MBR: 
MONBRDRAFT_13407 MONBRDRAFT_18816
TCR: 
503745.30 506193.60
LMA: 
LMJF_34_0070(APX)
LIF: 
LINJ_34_0070(APX)
LDO: 
LDBPK_340070
LMI: 
LMXM_33_0070
LBZ: 
LBRM_20_0150(APX)
PTI: 
PHATRDRAFT_13174 PHATRDRAFT_46616 PHATRDRAFT_47395
TPS: 
THAPSDRAFT_262753(ApxS) THAPSDRAFT_262809 THAPSDRAFT_38724
 » show all
Taxonomy
Reference
1  [PMID:6772104]
  Authors
Shigeoka S, Nakano Y, Kitaoka S.
  Title
Purification and some properties of L-ascorbic-acid-specific peroxidase in Euglena gracilis Z.
  Journal
Arch. Biochem. Biophys. 201 (1980) 121-7.
  Organism
Euglena gracilis
Reference
2  [PMID:6768357]
  Authors
Shigeoka S, Nakano Y, Kitaoka S.
  Title
Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase.
  Journal
Biochem. J. 186 (1980) 377-80.
  Organism
Euglena gracilis
Reference
3
  Authors
Nakano, Y and Asada, K.
  Title
Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical.
  Journal
Plant Cell Physiol. 28 (1987) 131-140.
Reference
4  [PMID:7703247]
  Authors
Patterson WR, Poulos TL
  Title
Crystal structure of recombinant pea cytosolic ascorbate peroxidase.
  Journal
Biochemistry. 34 (1995) 4331-41.
Reference
5  [PMID:15236572]
  Authors
Sharp KH, Moody PC, Brown KA, Raven EL
  Title
Crystal structure of the ascorbate peroxidase-salicylhydroxamic acid complex.
  Journal
Biochemistry. 43 (2004) 8644-51.
Reference
6  [PMID:16784232]
  Authors
Macdonald IK, Badyal SK, Ghamsari L, Moody PC, Raven EL
  Title
Interaction of ascorbate peroxidase with substrates: a mechanistic and structural analysis.
  Journal
Biochemistry. 45 (2006) 7808-17.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
72906-87-7
DBGET integrated database retrieval system