3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase;
steroid 4,5-dioxygenase;
3-alkylcatechol 2,3-dioxygenase

Oxidoreductases;
Acting on single donors with O2 as oxidant and incorporation of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O2;
With incorporation of two atoms of oxygen

3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (decyclizing)

3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [RN:R04597]

R04597

3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
O2 [CPD:C00007]

Requires Fe2+. Also acts on 3-isopropylcatechol and 3-tert-butyl-5-methylcatechol.

1  [PMID:5908121]

Gibson DT, Wang KC, Sih CJ, Whitlock H Jr.

Mechanisms of steroid oxidation by microorganisms. IX. On the mechanism of ring A cleavage in the degradation of 9,10-seco steroids by microorganisms.

J. Biol. Chem. 241 (1966) 551-9.

Nacardia restrictus