Renilla-luciferin 2-monooxygenase;
Renilla-type luciferase;
aequorin;
luciferase (Renilla luciferin)

Oxidoreductases;
Acting on single donors with O2 as oxidant and incorporation of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O2;
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating)

Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + hnu [RN:R03137]

R03137

Renilla luciferin [CPD:C00982];
O2 [CPD:C00007]

From the soft coral coelenterate Renilla reniformis. The luciferin is bound to a luciferin-binding protein (BP-LH2). The bioluminescent reaction between the luciferin complex, luciferase and oxygen is triggered by calcium ions. In vivo, the excited state luciferin---luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, Renilla green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein.

1  [PMID:4154104]

Cormier MJ, Hori K, Anderson JM.

Bioluminescence in coelenterates.

Biochim. Biophys. Acta. 346 (1974) 137-64.

Renilla reniformis

2  [PMID:237531]

Hori K, Anderson JM, Ward WW, Cormier MJ.

Renilla luciferin as the substrate for calcium induced photoprotein bioluminescence. Assignment of luciferin tautomers in aequorin and mnemiopsin.

Biochemistry. 14 (1975) 2371-6.

Renilla reniformis

3  [PMID:236561]

Shimomura O, Johnson FH.

Chemical nature of bioluminescence systems in coelenterates.

Proc. Natl. Acad. Sci. U. S. A. 72 (1975) 1546-9.

Renilla reniformis

4  [PMID:33174]

Charbonneau H, Cormier MJ.

Ca2+-induced bioluminescence in Renilla reniformis. Purification and characterization of a calcium-triggered luciferin-binding protein.

J. Biol. Chem. 254 (1979) 769-80.

Renilla reniformis

5  [PMID:4149963]

Anderson JM, Charbonneau H, Cormier MJ.

Mechanism of calcium induction of Renilla bioluminescence. Involvement of a calcium-triggered luciferin binding protein.

Biochemistry. 13 (1974) 1195-200.

Renilla reniformis, Renilla muelleri

6  [PMID:1674607]

Lorenz WW, McCann RO, Longiaru M, Cormier MJ.

Isolation and expression of a cDNA encoding Renilla reniformis luciferase.

Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 4438-42.

Renilla reniformis