KEGG   ENZYME: 1.13.12.5Help
Entry
EC 1.13.12.5                Enzyme                                 

Name
Renilla-luciferin 2-monooxygenase;
Renilla-type luciferase;
aequorin;
luciferase (Renilla luciferin)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
BRITE hierarchy
Sysname
Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating)
Reaction(IUBMB)
Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + hnu [RN:R03137]
Reaction(KEGG)
Substrate
Renilla luciferin [CPD:C00982];
O2 [CPD:C00007]
Product
oxidized Renilla luciferin [CPD:C03717];
CO2 [CPD:C00011];
hnu [CPD:C00205]
Comment
From the soft coral coelenterate Renilla reniformis. The luciferin is bound to a luciferin-binding protein (BP-LH2). The bioluminescent reaction between the luciferin complex, luciferase and oxygen is triggered by calcium ions. In vivo, the excited state luciferin---luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, Renilla green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein.
History
EC 1.13.12.5 created 1976, modified 1981, modified 1982, modified 2004
Orthology
K18053  
renilla-luciferin 2-monooxygenase
Genes
BFO: 
CIN: 
SPU: 
TAD: 
Taxonomy
Reference
1  [PMID:4154104]
  Authors
Cormier MJ, Hori K, Anderson JM.
  Title
Bioluminescence in coelenterates.
  Journal
Biochim. Biophys. Acta. 346 (1974) 137-64.
  Organism
Renilla reniformis
Reference
2  [PMID:237531]
  Authors
Hori K, Anderson JM, Ward WW, Cormier MJ.
  Title
Renilla luciferin as the substrate for calcium induced photoprotein bioluminescence. Assignment of luciferin tautomers in aequorin and mnemiopsin.
  Journal
Biochemistry. 14 (1975) 2371-6.
  Organism
Renilla reniformis
Reference
3  [PMID:236561]
  Authors
Shimomura O, Johnson FH.
  Title
Chemical nature of bioluminescence systems in coelenterates.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 72 (1975) 1546-9.
  Organism
Renilla reniformis
Reference
4  [PMID:33174]
  Authors
Charbonneau H, Cormier MJ.
  Title
Ca2+-induced bioluminescence in Renilla reniformis. Purification and characterization of a calcium-triggered luciferin-binding protein.
  Journal
J. Biol. Chem. 254 (1979) 769-80.
  Organism
Renilla reniformis
Reference
5  [PMID:4149963]
  Authors
Anderson JM, Charbonneau H, Cormier MJ.
  Title
Mechanism of calcium induction of Renilla bioluminescence. Involvement of a calcium-triggered luciferin binding protein.
  Journal
Biochemistry. 13 (1974) 1195-200.
  Organism
Renilla reniformis, Renilla muelleri
Reference
6  [PMID:1674607]
  Authors
Lorenz WW, McCann RO, Longiaru M, Cormier MJ.
  Title
Isolation and expression of a cDNA encoding Renilla reniformis luciferase.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 4438-42.
  Organism
Renilla reniformis
  Sequence
[up:P27652]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
61869-41-8

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