| Entry |
|
|---|
| Name |
leucocyanidin oxygenase;
anthocyanidin synthase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With 2-oxoglutarate as one donor, and incorporation of one atom of
oxygen into each donor
 |
|---|
| Sysname |
leucocyanidin,2-oxoglutarate:oxygen oxidoreductase |
|---|
| Reaction(IUBMB) |
leucocyanidin + 2-oxoglutarate + O2 = cis- and
trans-dihydroquercetins + succinate + CO2 + 2 H2O [RN:R05723 R07366] |
|---|
| Reaction(KEGG) |
R05723 R07366;
(other) R04276 R05036 R05037 R06540
 |
|---|
| Substrate |
leucocyanidin [CPD:C05906];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007] |
|---|
| Product |
cis-dihydroquercetin [CPD:C12316];
trans-dihydroquercetin [CPD:C01617];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001] |
|---|
| Comment |
The enzyme requires Fe(II) and ascorbate. It is involved in the
pathway by which many flowering plants make anthocyanin
(glycosylated anthocyandin) flower pigments. The intermediates are
transformed into cis- and trans-dihydroquercetin [2], which the
enzyme can also oxidize to quercetin. Acidification of the products
gives anthocyanidin [1], which, however, may not be a natural
precursor of the anthocyanins. |
|---|
| Pathway |
PATH: ec00941 Flavonoid biosynthesis
PATH: ec01061 Biosynthesis of phenylpropanoids
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K05277 leucoanthocyanidin dioxygenase |
|---|
| Genes |
ATH: AT4G22870 AT4G22880(LDOX)
OSA: 4325716(Os01g0372500)
 |
|---|
| Structures |
PDB: 1GP4 1GP5 2BRT |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:10074715]
Saito K, Kobayashi M, Gong Z, Tanaka Y, Yamazaki M.
Direct evidence for anthocyanidin synthase as a
2-oxoglutarate-dependent oxygenase: molecular cloning and functional
expression of cDNA from a red forma of Perilla frutescens.
Plant. J. 17 (1999) 181-9.
Perilla frutescens |
|---|
Reference
Authors
Title
Journal
|
2
Turnbull, J.J., Sobey, W.J., Aplin, R.T., Hassan, A., Firmin, J.L.,
Schofield, C.J. and Prescott, A.G.
Are anthocyanidins the immediate products of anthocyanidin
synthase?.
Chem. Commun. (2000) 2473-2474. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.11.19
IUBMB Enzyme Nomenclature: 1.14.11.19
ExPASy - ENZYME nomenclature database: 1.14.11.19
BRENDA, the Enzyme Database: 1.14.11.19
CAS: 180984-01-4 |
|---|
