| Entry |
|
|---|
| Name |
flavonol synthase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With 2-oxoglutarate as one donor, and incorporation of one atom of
oxygen into each donor
 |
|---|
| Sysname |
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase |
|---|
| Reaction(IUBMB) |
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate +
CO2 + H2O [RN:R07368] |
|---|
| Reaction(KEGG) |
R07368 > R02160 R03126 R06539 R08082
 |
|---|
| Substrate |
dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007] |
|---|
| Product |
flavonol [CPD:C01495];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001] |
|---|
| Comment |
In addition to the desaturation of (2R,3R)-dihydroflavonols to
flavonols, the enzyme from the satsuma Citrus unshiu also has a
non-specific activity that trans-hydroxylates the flavanones
(2S)-naringenin and the unnatural (2R)-naringenin at C-3 to
kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires
Fe2+. |
|---|
| Pathway |
PATH: ec00941 Flavonoid biosynthesis
PATH: ec01061 Biosynthesis of phenylpropanoids
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K05278 flavonol synthase |
|---|
| Genes |
ATH: AT5G08640(FLS) AT5G63590(FLS3)
OSA: 4330843(Os02g0767300)
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:12180990]
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U.
Functional expression and mutational analysis of flavonol synthase
from Citrus unshiu.
Eur. J. Biochem. 269 (2002) 4134-42.
Citrus unshiu |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:12620339]
Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.
Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.
Phytochemistry. 62 (2003) 287-92.
Citrus unshiu |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:12782296]
Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R.
Divergent evolution of flavonoid 2-oxoglutarate-dependent
dioxygenases in parsley.
FEBS. Lett. 544 (2003) 93-8.
Citrus unshiu, Eustoma grandiflorum, Petunia hybrida, Solanum
tuberosum |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:14570878]
Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield
CJ.
Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of
flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase,
and flavanone 3beta-hydroxylase.
J. Biol. Chem. 279 (2004) 1206-16.
Arabidopsis thaliana [GN:ath] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.11.23
IUBMB Enzyme Nomenclature: 1.14.11.23
ExPASy - ENZYME nomenclature database: 1.14.11.23
BRENDA, the Enzyme Database: 1.14.11.23
CAS: 146359-76-4 |
|---|
