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Entry
EC 1.14.11.34               Enzyme                                 

Name
2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming);
ethylene-forming enzyme;
EFE
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
L-arginine,2-oxoglutarate:oxygen oxidoreductase (succinate-forming)
Reaction(IUBMB)
2-oxoglutarate + L-arginine + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O (overall reaction) [RN:R09785];
(1a) 2-oxoglutarate + L-arginine + O2 = succinate + CO2 + L-hydroxyarginine [RN:R10081];
(1b) L-hydroxyarginine = guanidine + (S)-1-pyrroline-5-carboxylate + H2O [RN:R10082]
Reaction(KEGG)
Substrate
2-oxoglutarate [CPD:C00026];
L-arginine [CPD:C00062];
O2 [CPD:C00007];
L-hydroxyarginine [CPD:C20330]
Product
succinate [CPD:C00042];
CO2 [CPD:C00011];
guanidine [CPD:C17349];
(S)-1-pyrroline-5-carboxylate [CPD:C03912];
H2O [CPD:C00001];
L-hydroxyarginine [CPD:C20330]
Comment
This is one of two simultaneous reactions catalysed by the enzyme, which is responsible for ethylene production in bacteria of the Pseudomonas syringae group.
In the other reaction [EC 1.13.12.19, 2-oxoglutarate dioxygenase (ethylene-forming)] the enzyme catalyses the dioxygenation of 2-oxoglutarate forming ethylene and three molecules of carbon dioxide.
The enzyme catalyses two cycles of the ethylene-forming reaction for each cycle of the succinate-forming reaction, so that the stoichiometry of the products ethylene and succinate is 2:1.
History
EC 1.14.11.34 created 2011
Reference
1  [PMID:1770346]
  Authors
Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, Morino Y, Fukuda H
  Title
Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2.
  Journal
J. Gen. Microbiol. 137 (1991) 2281-6.
  Organism
Pseudomonas syringae
  Sequence
[up:P32021]
Reference
2  [PMID:1445291]
  Authors
Fukuda H, Ogawa T, Tazaki M, Nagahama K, Fujii T, Tanase S, Morino Y
  Title
Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae.
  Journal
Biochem. Biophys. Res. Commun. 188 (1992) 483-9.
  Organism
Pseudomonas syringae
  Sequence
[up:P32021]
Reference
3  [PMID:1445325]
  Authors
Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, Omata T, Inoue Y, Tanase S, Morino Y
  Title
Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2.
  Journal
Biochem. Biophys. Res. Commun. 188 (1992) 826-32.
  Organism
Pseudomonas syringae
  Sequence
[up:P32021]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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