KEGG   ENZYME: 1.14.11.35Help
Entry
EC 1.14.11.35               Enzyme                                 

Name
1-deoxypentalenic acid 11beta-hydroxylase;
ptlH (gene name);
sav2991 (gene name);
pntH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
1-deoxypentalenate + 2-oxoglutarate + O2 = 1-deoxy-11beta-hydroxypentalenate + succinate + CO2 [RN:R10157]
Reaction(KEGG)
Substrate
1-deoxypentalenate [CPD:C20404];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
1-deoxy-11beta-hydroxypentalenate [CPD:C20403];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
History
EC 1.14.11.35 created 2012
Orthology
K18056  
1-deoxypentalenic acid 11beta-hydroxylase
Genes
SMA: 
SAV_2991(ptlH)
SBH: 
SCI: 
Taxonomy
Reference
1  [PMID:16704250]
  Authors
You Z, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis.
  Journal
J. Am. Chem. Soc. 128 (2006) 6566-7.
  Sequence
[sma:SAV_2991]
Reference
2  [PMID:17942405]
  Authors
You Z, Omura S, Ikeda H, Cane DE, Jogl G
  Title
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis.
  Journal
J. Biol. Chem. 282 (2007) 36552-60.
  Sequence
[sma:SAV_2991]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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