KEGG   ENZYME: 1.14.12.8Help
Entry
EC 1.14.12.8                Enzyme                                 
Name
4-sulfobenzoate 3,4-dioxygenase;
4-sulfobenzoate dioxygenase;
4-sulfobenzoate 3,4-dioxygenase system
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
4-sulfobenzoate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
Reaction(IUBMB)
4-sulfobenzoate + NADH + H+ + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+ [RN:R01636]
Reaction(KEGG)
Substrate
4-sulfobenzoate [CPD:C02236];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxybenzoate [CPD:C00230];
sulfite [CPD:C00094];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FMN), an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+.
Pathway
Toluene degradation
Microbial metabolism in diverse environments
Reference
1  [PMID:2012609]
  Authors
Locher HH, Leisinger T, Cook AM.
  Title
4-Sulphobenzoate 3,4-dioxygenase. Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2.
  Journal
Biochem. J. 274 ( Pt 3) (1991) 833-42.
  Organism
Comamonas testosteroni
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
122933-81-7
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