KEGG   ENZYME: 1.14.13.100Help
Entry
EC 1.14.13.100              Enzyme                                 

Name
25/26-hydroxycholesterol 7alpha-hydroxylase;
25-hydroxycholesterol 7alpha-monooxygenase;
CYP7B1;
CYP7B1 oxysterol 7alpha-hydroxylase;
27-hydroxycholesterol 7-monooxygenase;
27-hydroxycholesterol 7alpha-hydroxylase;
cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating);
25-hydroxycholesterol 7alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
cholest-5-ene-3beta,25/26-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
Reaction(IUBMB)
(1) cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 = cholest-5-ene-3beta,7alpha,25-triol + NADP+ + H2O [RN:R07209];
(2) (25R)-cholest-5-ene-3beta,26-diol + NADPH + H+ + O2 = (25R)-cholest-5-ene-3beta,7alpha,26-triol + NADP+ + H2O [RN:R07372]
Reaction(KEGG)
Substrate
cholest-5-ene-3beta,25-diol [CPD:C15519];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
(25R)-cholest-5-ene-3beta,26-diol
Product
cholest-5-ene-3beta,7alpha,25-triol [CPD:C15520];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
(25R)-cholest-5-ene-3beta,7alpha,26-triol
Comment
A heme-thiolate protein (P-450). Unlike EC 1.14.13.99, 24-hydroxycholesterol 7alpha-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2].
History
EC 1.14.13.100 created 2005, modified 2013 (EC 1.14.13.60 created 1999, incorporated 2013)
Pathway
Primary bile acid biosynthesis
Steroid hormone biosynthesis
Orthology
K07430  
oxysterol 7-alpha-hydroxylase
Genes
HSA: 
9420(CYP7B1)
PTR: 
743817(CYP7B1)
PPS: 
GGO: 
PON: 
NLE: 
MCC: 
MCF: 
CJC: 
100385287(CYP7B1)
MMU: 
13123(Cyp7b1)
RNO: 
25429(Cyp7b1)
CGE: 
HGL: 
TUP: 
CFA: 
486973(CYP7B1)
AML: 
UMR: 
FCA: 
PTG: 
BTA: 
529552(CYP7B1)
BOM: 
PHD: 
CHX: 
OAS: 
SSC: 
100286867(CYP7B1)
CFR: 
BACU: 
LVE: 
ECB: 
MYB: 
MYD: 
PALE: 
MDO: 
100029487(CYP7B1)
SHR: 
OAA: 
GGA: 
420164(CYP7B1)
MGP: 
APLA: 
TGU: 
FAB: 
PHI: 
FPG: 
FCH: 
CLV: 
ASN: 
AMJ: 
PSS: 
CMY: 
ACS: 
PBI: 
XTR: 
100145642(cyp7b1)
DRE: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
CIN: 
BZE: 
BSC: 
BOR: 
PSQ: 
SLA: 
 » show all
Taxonomy
Reference
1
  Authors
Kumiko, O.M., Budai, K. and Javitt, N.B.
  Title
Cholesterol and 27-hydroxycholesterol 7alpha-hydroxylation: evidence for two different enzymes.
  Journal
J. Lipid Res. 34 (1993) 581-588.
Reference
2  [PMID:7925343]
  Authors
Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Bjorkhem I.
  Title
7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase.
  Journal
Eur. J. Biochem. 224 (1994) 309-16.
Reference
3  [PMID:10748047]
  Authors
Li-Hawkins J, Lund EG, Bronson AD, Russell DW.
  Title
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol.
  Journal
J. Biol. Chem. 275 (2000) 16543-9.
Reference
4  [PMID:12759897]
  Authors
Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM.
  Title
Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat.
  Journal
Metabolism. 52 (2003) 636-42.
  Sequence
[rno:25429]
Reference
5  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu. Rev. Biochem. 72 (2003) 137-74.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
149316-80-3

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