KEGG   ENZYME: 1.14.13.17Help
Entry
EC 1.14.13.17               Enzyme                                 
Name
cholesterol 7alpha-monooxygenase;
cholesterol 7alpha-hydroxylase;
CYP7A1
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
cholesterol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
Reaction(IUBMB)
cholesterol + NADPH + H+ + O2 = 7alpha-hydroxycholesterol + NADP+ + H2O [RN:R01463]
Reaction(KEGG)
R01463 > R07372;
(other) R08961
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Substrate
cholesterol [CPD:C00187];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
7alpha-hydroxycholesterol [CPD:C03594];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450).
Pathway
Primary bile acid biosynthesis
Steroid hormone biosynthesis
Metabolic pathways
Orthology
K00489  
cytochrome P450, family 7, subfamily A (cholesterol 7alpha-monooxygenase)
Genes
HSA: 
1581(CYP7A1)
PTR: 
464191(CYP7A1)
PPS: 
GGO: 
PON: 
MCC: 
698127(CYP7A1)
MMU: 
13122(Cyp7a1)
RNO: 
25428(Cyp7a1)
CFA: 
486962(CYP7A1)
AML: 
FCA: 
101099612
BTA: 
510507(CYP7A1)
SSC: 
448985(CYP7A1)
ECB: 
100052888
MDO: 
100022261(CYP7A1)
SHR: 
100926104
OAA: 
100079392
GGA: 
414834(CYP7A1)
MGP: 
100539035
TGU: 
100225999
ACS: 
100562614
XTR: 
100488670
DRE: 
394148(cyp7a1a)
TRU: 
101069556
OLA: 
101160349
SSL: 
SS1G_14166
BFU: 
BC1G_07665
 » show all
Taxonomy
Reference
1  [PMID:4147676]
  Authors
Boyd GS, Grimwade AM, Lawson ME.
  Title
Studies on rat-liver microsomal cholesterol 7alpha-hydroxylase.
  Journal
Eur. J. Biochem. 37 (1973) 334-40.
  Organism
Rattus norvegicus [GN:rno]
Reference
2  [PMID:4397276]
  Authors
Mitton JR, Scholan NA, Boyd GS.
  Title
The oxidation of cholesterol in rat liver sub-cellular particles. The cholesterol-7-alpha-Hydroxylase enzyme system.
  Journal
Eur. J. Biochem. 20 (1971) 569-79.
  Organism
Rattus norvegicus [GN:rno]
Reference
3  [PMID:3584134]
  Authors
Ogishima T, Deguchi S, Okuda K.
  Title
Purification and characterization of cholesterol 7 alpha-hydroxylase from rat liver microsomes.
  Journal
J. Biol. Chem. 262 (1987) 7646-50.
  Organism
Rattus norvegicus [GN:rno]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9037-53-0
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