| Entry |
|
|---|
| Name |
melilotate 3-monooxygenase;
2-hydroxyphenylpropionate hydroxylase;
melilotate hydroxylase;
2-hydroxyphenylpropionic hydroxylase;
melilotic hydroxylase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase
(3-hydroxylating) |
|---|
| Reaction(IUBMB) |
3-(2-hydroxyphenyl)propanoate + NADH + H+ + O2 =
3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O [RN:R03369] |
|---|
| Reaction(KEGG) |
R03369
 |
|---|
| Substrate |
3-(2-hydroxyphenyl)propanoate [CPD:C01198];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
3-(2,3-dihydroxyphenyl)propanoate [CPD:C04044];
NAD+ [CPD:C00003];
H2O [CPD:C00001] |
|---|
| Cofactor |
FAD [CPD:C00016] |
|---|
| Comment |
A flavoprotein (FAD). |
|---|
| Pathway |
PATH: ec00360 Phenylalanine metabolism |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:6017743]
Levy CC.
Melilotate hydroxylase. Purification of the enzyme and the nature of
the prosthetic group.
J. Biol. Chem. 242 (1967) 747-53.
Arthrobacter sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:4285850]
Levy CC, Frost P.
The metabolism of coumarin by a microorganism. V. Melilotate
hydroxylase.
J. Biol. Chem. 241 (1966) 997-1003.
Arthrobacter sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:4348920]
Strickland S, Massey V.
The purification and properties of the flavoprotein melilotate
hydroxylase.
J. Biol. Chem. 248 (1973) 2944-52.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:4348921]
Strickland S, Massey V.
The mechanism of action of the flavoprotein melilotate hydroxylase.
J. Biol. Chem. 248 (1973) 2953-62.
Pseudomonas sp. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.4
IUBMB Enzyme Nomenclature: 1.14.13.4
ExPASy - ENZYME nomenclature database: 1.14.13.4
BRENDA, the Enzyme Database: 1.14.13.4
CAS: 37256-72-7 |
|---|
