KEGG   ENZYME: 1.14.13.52Help
Entry
EC 1.14.13.52               Enzyme                                 

Name
isoflavone 3'-hydroxylase;
isoflavone 3'-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
formononetin + NADPH + H+ + O2 = calycosin + NADP+ + H2O [RN:R03006]
Reaction(KEGG)
R03006;
(other) R07776
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Substrate
formononetin [CPD:C00858];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
calycosin [CPD:C01562];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Also acts on biochanin A and other isoflavones with a 4'-methoxy group. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain.
History
EC 1.14.13.52 created 1992
Pathway
Isoflavonoid biosynthesis
Biosynthesis of secondary metabolites
Reference
1
  Authors
Hinderer, W., Flentje, U. and Barz, W.
  Title
Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation.
  Journal
FEBS Lett. 214 (1987) 101-106.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110183-50-1

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