| Entry |
|
|---|
| Name |
taxane 10beta-hydroxylase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
taxa-4(20),11-dien-5alpha-yl acetate,NADPH:oxygen oxidoreductase
(10beta-hydroxylating) |
|---|
| Reaction(IUBMB) |
taxa-4(20),11-dien-5alpha-yl acetate + NADPH + H+ + O2 =
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O
[RN:R06309] |
|---|
| Reaction(KEGG) |
R06309
 |
|---|
| Substrate |
taxa-4(20),11-dien-5alpha-yl acetate;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C11898];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Comment |
This microsomal cytochrome-P450-dependent enzyme is involved in the
biosynthesis of the diterpenoid antineoplastic drug taxol
(paclitaxel). |
|---|
| Pathway |
PATH: ec00904 Diterpenoid biosynthesis |
|---|
| Orthology |
KO: K12924 taxane 10-beta-hydroxylase |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:11396929]
Wheeler AL, Long RM, Ketchum RE, Rithner CD, Williams RM, Croteau R.
Taxol biosynthesis: differential transformations of taxadien-5
alpha-ol and its acetate ester by cytochrome P450 hydroxylases from
Taxus suspension cells.
Arch. Biochem. Biophys. 390 (2001) 265-78.
Taxus sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:11707604]
Jennewein S, Rithner CD, Williams RM, Croteau RB.
Taxol biosynthesis: taxane 13 alpha-hydroxylase is a cytochrome
P450-dependent monooxygenase.
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 13595-600.
Taxus cuspidata |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:11171980]
Schoendorf A, Rithner CD, Williams RM, Croteau RB.
Molecular cloning of a cytochrome P450 taxane 10 beta-hydroxylase
cDNA from Taxus and functional expression in yeast.
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 1501-6.
Taxus sp. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.76
IUBMB Enzyme Nomenclature: 1.14.13.76
ExPASy - ENZYME nomenclature database: 1.14.13.76
BRENDA, the Enzyme Database: 1.14.13.76
CAS: 337514-75-7 |
|---|
