| Entry |
|
|---|
| Name |
ent-kaurenoic acid oxidase |
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| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
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| Sysname |
ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating) |
|---|
| Reaction(IUBMB) |
(1) (1a) ent-kaur-16-en-19-oate + NADPH + H+ + O2 =
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O [RN:R06294];
(2) (1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2 =
gibberellin A12 aldehyde + NADP+ + 2 H2O [RN:R06295];
(3) (1c) gibberellin A12 aldehyde + NADPH + O2 = gibberellin A12 +
NADP+ + H2O [RN:R06297] |
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| Reaction(KEGG) |
R06294 R06295 R06297;
(other) R06296
 |
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| Substrate |
ent-kaur-16-en-19-oate [CPD:C11874];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
ent-7alpha-hydroxykaur-16-en-19-oate [CPD:C11875];
gibberellin A12 aldehyde [CPD:C06093] |
|---|
| Product |
ent-7alpha-hydroxykaur-16-en-19-oate [CPD:C11875];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
gibberellin A12 aldehyde [CPD:C06093];
gibberellin A12 [CPD:C11857] |
|---|
| Comment |
Requires cytochrome P450. Catalyses three sucessive oxidations of
ent-kaurenoic acid. The second step includes a ring-B contraction
giving the gibbane skeleton. In pumpkin (Cucurbita maxima)
ent-6alpha,7alpha-dihydroxykaur-16-en-19-oate is also formed. |
|---|
| Pathway |
PATH: ec00904 Diterpenoid biosynthesis
PATH: ec01062 Biosynthesis of terpenoids and steroids
PATH: ec01070 Biosynthesis of plant hormones
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K04123 ent-kaurenoic acid hydroxylase |
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| Genes |
ATH: AT1G05160 AT2G32440(KAO2)
OSA: 4339885(Os06g0110000)
 |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:11172076]
Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ.
The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes
three steps of the gibberellin biosynthesis pathway.
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 2065-70.
Zea mays [GN:zma] |
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| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.79
IUBMB Enzyme Nomenclature: 1.14.13.79
ExPASy - ENZYME nomenclature database: 1.14.13.79
BRENDA, the Enzyme Database: 1.14.13.79
CAS: 337507-95-6 |
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