KEGG   ENZYME: 1.14.13.81Help
Entry
EC 1.14.13.81               Enzyme                                 
Name magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
Class Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
BRITE hierarchy
Sysname magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen
oxidoreductase (hydroxylating)
Reaction(IUBMB) (1) (1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH +
H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl
ester + NADP+ + H2O [RN:R06265];
(2) (1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester
+ NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX
13-monomethyl ester + NADP+ + 2 H2O [RN:R06266];
(3) (1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester +
NADPH + H+ + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O
[RN:R06267]
Reaction(KEGG) R06265 R06266 R06267
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Substrate magnesium-protoporphyrin IX 13-monomethyl ester [CPD:C04536];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Product 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
NADP+ [CPD:C00006];
H2O [CPD:C00001];
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester;
divinylprotochlorophyllide [CPD:C11831]
Comment Requires Fe(II) for activity. The cyclase activity in Chlamydomonas
reinhardtii is associated exclusively with the membranes, whereas
that from cucumber cotyledons requires both membrane and soluble
fractions for activity.
Pathway PATH: ec00860  Porphyrin and chlorophyll metabolism
PATH: ec01100  Metabolic pathways
Orthology KO: K04035  magnesium-protoporphyrin IX monomethyl ester (oxidative)
            cyclase
Genes ATH: AT3G56940(CRD1)
OSA: 4326901(Os01g0279100)
OLU: OSTLU_47501
NOC: Noc_1738
HHA: Hhal_1625
BUR: Bcep18194_A4283
AZO: azo0271
PPD: Ppro_2479 Ppro_2577 Ppro_2638 Ppro_3390
DVL: Dvul_0353
SFU: Sfum_0909
BRA: BRADO1649(acsF)
BBT: BBta_6407(acsF)
RPA: RPA1552
RPB: RPB_2687 RPB_3863 RPB_3971
RPC: RPC_1323 RPC_3812
RPD: RPD_1487 RPD_3726
RPE: RPE_1357
RPT: Rpal_1740
MEX: Mext_4806
MDI: METDI5863(acsF)
MRD: Mrad2831_1838
MET: M446_3728
MPO: Mpop_5351
MCH: Mchl_5273
MSL: Msil_2032
RSP: RSP_0294
RSH: Rsph17029_1938
RSQ: Rsph17025_1000
JAN: Jann_0151
RDE: RD1_0129(acsF)
DSH: Dshi_3544(acsF)
RCE: RC1_2105(acsF)
FRA: Francci3_1863 Francci3_2496
SYN: sll1214 sll1874(AT103) slr0905(bchE)
SYW: SYNW1198(PNIL34)
SYC: syc2188_d(ycf59)
SYF: Synpcc7942_1907
SYD: Syncc9605_1310
SYE: Syncc9902_1165
SYG: sync_1304(acsF)
SYR: SynRCC307_1476(acsF)
SYX: SynWH7803_1102(acsF)
SYP: SYNPCC7002_A0707(acsF) SYNPCC7002_A1992(acsF)
CYA: CYA_2703(acsF)
CYB: CYB_1254(acsF)
TEL: tlr1426(ycf59) tlr1722
MAR: MAE_39240 MAE_46620
CYT: cce_2967 cce_4412
CYP: PCC8801_2261 PCC8801_3705
CYC: PCC7424_2605 PCC7424_3134
CYN: Cyan7425_4340 Cyan7425_4781
GVI: gvip493(ycf59)
ANA: all1880 alr1358 alr3300
NPU: Npun_F1653
AVA: Ava_1133 Ava_4776 Ava_4958
PMA: Pro0992(acsF)
PMM: PMM0844(PNIL34)
PMT: PMT2196
PMN: PMN2A_0227
PMI: PMT9312_0956
PMB: A9601_10251(pniL34)
PMC: P9515_09201(pnilL34)
PMF: P9303_29201(pniL34)
PMG: P9301_10241(pniL34)
PMH: P9215_10571(pniL34)
PME: NATL1_08941(pnil34)
TER: Tery_0728
AMR: AM1_0465(acsF)
CCH: Cag_0114 Cag_0229 Cag_0931 Cag_1537
CPH: Cpha266_2315 Cpha266_2592
PVI: Cvib_0316 Cvib_1454
PLT: Plut_0251 Plut_1668
RRS: RoseRS_1905
RCA: Rcas_1541
CAU: Caur_2590
CAG: Cagg_1285
CHL: Chy400_2799
MBU: Mbur_1602
SMR: Smar_0664
Taxonomy
Reference
  Authors
  Title


  Journal
  Organism
 1  [PMID:12226378]
Bollivar DW, Beale SI.
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl
Ester (Oxidative) Cyclase (Characterization and Partial Purification
from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803).
Plant. Physiol. 112 (1996) 105-114.
Chlamydomonas reinhardtii [GN:cre], Synechocystis sp. [GN:syn]
Other DBs ExplorEnz - The Enzyme Database: 1.14.13.81
IUBMB Enzyme Nomenclature: 1.14.13.81
ExPASy - ENZYME nomenclature database: 1.14.13.81
BRENDA, the Enzyme Database: 1.14.13.81
CAS: 92353-62-3
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