| Entry |
|
|---|
| Name |
precorrin-3B synthase;
precorrin-3X synthase;
CobG |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
precorrin-3A,NADH:oxygen oxidoreductase (20-hydroxylating) |
|---|
| Reaction(IUBMB) |
precorrin-3A + NADH + H+ + O2 = precorrin-3B + NAD+ + H2O
[RN:R05217] |
|---|
| Reaction(KEGG) |
R05217
 |
|---|
| Substrate |
precorrin 3A [CPD:C05772];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
precorrin 3B [CPD:C06406];
NAD+ [CPD:C00003];
H2O [CPD:C00001] |
|---|
| Comment |
An iron-sulfur protein. An oxygen atom from dioxygen is incorporated
into the macrocycle at C-20. In the aerobic cobalamin biosythesis
pathway, four enzymes are involved in the conversion of precorrin-3A
to precorrin-6A. The first of the four steps is carried out by EC
1.14.13.83, precorrin-3B synthase (CobG), yielding precorrin-3B as
the product. This is followed by three methylation reactions, which
introduce a methyl group at C-17 (CobJ; EC 2.1.1.131), C-11 (CobM;
EC 2.1.1.133) and C-1 (CobF; EC 2.1.1.152) of the macrocycle, giving
rise to precorrin-4, precorrin-5 and precorrin-6A, respectively. |
|---|
| Pathway |
PATH: ec00860 Porphyrin and chlorophyll metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K02229 precorrin-3B synthase |
|---|
| Genes |
PAE: PA2906
PAU: PA14_26485
PAP: PSPA7_2248(cobG)
PAG: PLES_21581
PPU: PP_4829
PPF: Pput_4707
PPG: PputGB1_4885
PPW: PputW619_4619
PST: PSPTO_4877
PSB: Psyr_4417
PSP: PSPPH_4460
PFL: PFL_0657(nirA)
PFO: Pfl01_0604
PFS: PFLU0604
PEN: PSEEN4871(cobG)
PMY: Pmen_4571
BMA: BMA1161
BMV: BMASAVP1_A1602
BML: BMA10229_A0264
BMN: BMA10247_0896(cobG)
BPS: BPSL1759
BPM: BURPS1710b_2113(cobG)
BPL: BURPS1106A_1967(cobG)
BPD: BURPS668_1950(cobG)
BPR: GBP346_A1991(cobG)
BTE: BTH_I2398
BVI: Bcep1808_1610
BUR: Bcep18194_A4824
BCN: Bcen_1194
BCH: Bcen2424_1674
BCM: Bcenmc03_1647
BCJ: BCAL1726
BAM: Bamb_1574
BAC: BamMC406_1592
BMU: Bmul_1568
BMJ: BMULJ_01676(cobG)
BXE: Bxe_B1245
BPH: Bphy_3143
BPY: Bphyt_5369
BGL: bglu_1g18430
DAC: Daci_5851
CTT: CtCNB1_1878
MLO: mlr1378
SME: SMc03193(cobG)
SMD: Smed_2818
ATU: Atu2803(cobG)
ATC: AGR_C_5083(cobG)
ARA: Arad_9596(cobG)
RET: RHE_PE00455(cobG)
REC: RHECIAT_PA0000378(cobG)
RLE: pRL110631(cobG)
RLT: Rleg2_6019
RLG: Rleg_7174
RHI: NGR_c29810(cobG)
BME: BMEI0715
BMF: BAB1_1304
BMB: BruAb1_1287
BMC: BAbS19_I12170
BMS: BR1286
BMT: BSUIS_A1336(cobG)
BOV: BOV_1249(cobG)
BCS: BCAN_A1309(cobG)
BMR: BMI_I1299(cobG)
OAN: Oant_1899
BJA: blr3264
BRA: BRADO4906
BBT: BBta_3145
XAU: Xaut_3285
AZC: AZC_3936(cobG)
MEX: Mext_1438
MEA: Mex_1p1327(cobG)
MDI: METDI2099(cobG)
MRD: Mrad2831_3116
MET: M446_2439
MPO: Mpop_1435
MCH: Mchl_1713
MNO: Mnod_2150
BID: Bind_3508
MSL: Msil_3267
SIL: SPO2864
SIT: TM1040_2211
JAN: Jann_2930
RDE: RD1_3826(cobG)
PDE: Pden_2533
NAR: Saro_0341
GBE: GbCGDNIH1_0659
APT: APA01_14560(cobG)
MTU: Rv2064(cobG)
MTC: MT2124
MRA: MRA_2078(cobG)
MTF: TBFG_12100
MTB: TBMG_01917(TBMG_01917.1)
MBO: Mb2090(cobG)
MBB: BCG_2083(cobG)
MBT: JTY_2077(cobG)
MPA: MAP1810(cobG)
MSM: MSMEG_3871(cobG)
MUL: MUL_2288
MVA: Mvan_3429
MGI: Mflv_3106
MAB: MAB_2200c
MMC: Mmcs_2489
MKM: Mkms_2534
MJL: Mjls_2526
MMI: MMAR_3044
CDI: DIP1231
CAR: cauri_1273(cobG)
CKP: ckrop_0898(cobG)
NFA: nfa31400
RHA: RHA1_ro00172
RER: RER_31420(cobG)
ROP: ROP_02920(cobG)
GBR: Gbro_2481
SGR: SGR_3824
TFU: Tfu_0318
SRO: Sros_5592
FRA: Francci3_1519
FAL: FRAAL2331(cobG)
NML: Namu_0151
SEN: SACE_5953
SVI: Svir_17650
AMI: Amir_1783
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:8226690]
Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F.
Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas
denitrificans.
J. Bacteriol. 175 (1993) 7430-40.
Pseudomonas denitrificans |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:8405386]
Scott AI, Roessner CA, Stolowich NJ, Spencer JB, Min C, Ozaki SI.
Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative
ring contraction and insertion of the fourth methyl group.
FEBS. Lett. 331 (1993) 105-8.
Pseudomonas denitrificans |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:12195810]
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
The biosynthesis of adenosylcobalamin (vitamin B12).
Nat. Prod. Rep. 19 (2002) 390-412.
Pseudomonas denitrificans |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.83
IUBMB Enzyme Nomenclature: 1.14.13.83
ExPASy - ENZYME nomenclature database: 1.14.13.83
BRENDA, the Enzyme Database: 1.14.13.83
CAS: 152787-63-8 |
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