KEGG   ENZYME: 1.14.13.93Help
Entry
EC 1.14.13.93               Enzyme                                 

Name
(+)-abscisic acid 8'-hydroxylase;
(+)-ABA 8'-hydroxylase;
ABA 8'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
abscisate,NADPH:oxygen oxidoreductase (8'-hydroxylating)
Reaction(IUBMB)
(+)-abscisate + NADPH + H+ + O2 = 8'-hydroxyabscisate + NADP+ + H2O [RN:R07202]
Reaction(KEGG)
Substrate
(+)-abscisate;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
8'-hydroxyabscisate [CPD:C15514];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Catalyses the first step in the oxidative degradation of abscisic acid and is considered to be the pivotal enzyme in controlling the rate of degradation of this plant hormone [1]. CO inhibits the reaction, but its effects can be reversed by the presence of blue light [1]. The 8'-hydroxyabscisate formed can be converted into (-)-phaseic acid, most probably spontaneously. Other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.13.11.51 (9-cis-epoxycarotenoid dioxygenase).
History
EC 1.14.13.93 created 2005
Pathway
Carotenoid biosynthesis
Orthology
K09843  
(+)-abscisic acid 8'-hydroxylase
Genes
ATH: 
AT2G29090(CYP707A2) AT3G19270(CYP707A4) AT4G19230(CYP707A1) AT5G45340(CYP707A3)
ALY: 
CRB: 
EUS: 
CIT: 
102611561(CYP707A4) 102613470(CYP707A1) 102616423(CYP707A3) 102627021(CYP707A2)
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0001s24940g(POPTRDRAFT_797669) POPTR_0002s12770g(POPTRDRAFT_551854) POPTR_0004s14820g POPTR_0004s24360g POPTR_0009s03900g(POPTRDRAFT_723222) POPTR_0009s10450g(POPTRDRAFT_767572) POPTR_0014s02910g
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os02t0703600-01(Os02g0703600) Os08t0472800-01(Os08g0472800) Os09t0457100-01(Os09g0457100)
OBR: 
BDI: 
SBI: 
SORBI_02g026600(SORBIDRAFT_02g026600) SORBI_04g030660(SORBIDRAFT_04g030660) SORBI_07g022990(SORBIDRAFT_07g022990)
ZMA: 
100191697(IDP1977) 100274595(umc1299) 100383693
SITA: 
ATR: 
s00011p00261570(AMTR_s00011p00261570) s00091p00146660(AMTR_s00091p00146660)
SMO: 
 » show all
Taxonomy
Reference
1
  Authors
Cutler, A.J., Squires, T.M., Loewen, M.K. and Balsevich, J.J.
  Title
Induction of (+)-abscisic acid 8' hydroxylase by (+)-abscisic acid in cultured maize cells.
  Journal
J. Exp. Bot. 48 (1997) 1787-1795.
Reference
2  [PMID:9808729]
  Authors
Krochko JE, Abrams GD, Loewen MK, Abrams SR, Cutler AJ.
  Title
(+)-Abscisic acid 8'-hydroxylase is a cytochrome P450 monooxygenase
  Journal
Plant. Physiol. 118 (1998) 849-60.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
153190-37-5

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