KEGG   ENZYME: 1.14.13.99Help
Entry
EC 1.14.13.99               Enzyme                                 

Name
24-hydroxycholesterol 7alpha-hydroxylase;
24-hydroxycholesterol 7alpha-monooxygenase;
CYP39A1;
CYP39A1 oxysterol 7alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(24R)-cholest-5-ene-3beta,24-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
Reaction(IUBMB)
(24R)-cholest-5-ene-3beta,24-diol + NADPH + H+ + O2 = (24R)-cholest-5-ene-3beta,7alpha,24-triol + NADP+ + H2O [RN:R07208]
Reaction(KEGG)
Substrate
(24R)-cholest-5-ene-3beta,24-diol;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(24R)-cholest-5-ene-3beta,7alpha,24-triol;
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450) that is found in liver microsomes and in ciliary non-pigmented epithelium [2]. The enzyme is specific for (24R)-cholest-5-ene-3beta,24-diol as substrate.
History
EC 1.14.13.99 created 2005
Pathway
Primary bile acid biosynthesis
Orthology
K07439  
24-hydroxycholesterol 7alpha-hydroxylase
Genes
HSA: 
51302(CYP39A1)
PTR: 
462743(CYP39A1)
PPS: 
GGO: 
PON: 
MCC: 
704242(CYP39A1)
MCF: 
MMU: 
56050(Cyp39a1)
RNO: 
301264(Cyp39a1)
CGE: 
HGL: 
TUP: 
CFA: 
481825(CYP39A1)
AML: 
FCA: 
PTG: 
BTA: 
511195(CYP39A1)
BOM: 
PHD: 
CHX: 
SSC: 
100113410(CYP39A1)
CFR: 
BACU: 
LVE: 
ECB: 
MYB: 
MYD: 
PALE: 
MDO: 
SHR: 
OAA: 
GGA: 
422058(CYP39A1)
TGU: 
FAB: 
PHI: 
APLA: 
FPG: 
FCH: 
CLV: 
ASN: 
AMJ: 
PSS: 
CMY: 
ACS: 
XLA: 
733346(cyp39a1)
XTR: 
DRE: 
564475(cyp39a1)
LCM: 
CMK: 
BFO: 
TAD: 
AQU: 
CSL: 
CME: 
GSL: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:10748047]
  Authors
Li-Hawkins J, Lund EG, Bronson AD, Russell DW.
  Title
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol.
  Journal
J. Biol. Chem. 275 (2000) 16543-9.
  Organism
Mus musculus, Homo sapiens
  Sequence
[mmu:56050] [hsa:51302]
Reference
2  [PMID:12649335]
  Authors
Ikeda H, Ueda M, Ikeda M, Kobayashi H, Honda Y.
  Title
Oxysterol 7alpha-hydroxylase (CYP39A1) in the ciliary nonpigmented epithelium of bovine eye.
  Journal
Lab. Invest. 83 (2003) 349-55.
  Organism
Bos taurus
Reference
3  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu. Rev. Biochem. 72 (2003) 137-74.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
288309-90-0

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