| Entry |
|
|---|
| Name |
deacetoxycephalosporin-C synthase;
DAOCS;
penicillin N expandase;
DAOC synthase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With 2-oxoglutarate as one donor, and the other dehydrogenated
 |
|---|
| Sysname |
penicillin-N,2-oxoglutarate:oxygen oxidoreductase (ring-expanding) |
|---|
| Reaction(IUBMB) |
penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C +
succinate + CO2 + H2O [RN:R05301] |
|---|
| Reaction(KEGG) |
R05301
 |
|---|
| Substrate |
penicillin N [CPD:C06564];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007] |
|---|
| Product |
deacetoxycephalosporin C [CPD:C06565];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001] |
|---|
| Comment |
Forms part of the penicillin biosynthesis pathway (for pathway,
click here). |
|---|
| Pathway |
PATH: ec00311 Penicillin and cephalosporin biosynthesis
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K12744 deacetoxycephalosporin-C synthase
KO: K12746 cephalosporin biosynthesis expandase/hydroxylase |
|---|
| Structures |
PDB: 1UNB 1UO9 1UOB 1UOF 1UOG 1W28 1W2A 2JB8 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:1354366]
Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP.
Isolation of deacetoxycephalosporin C from fermentation broths of
Penicillium chrysogenum transformants: construction of a new fungal
biosynthetic pathway.
Proc. R. Soc. Lond. B. Biol. Sci. 248 (1992) 283-9.
Penicillium chrysogenum [GN:pcs] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:11352583]
Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ.
Kinetic and crystallographic studies on deacetoxycephalosporin C
synthase (DAOCS).
J. Mol. Biol. 308 (2001) 937-48.
Streptomyces clavuligerus |
|---|
Reference
Authors
Title
Journal
|
3
Yeh, W.K., Ghag, S.K. and Queener, S.W.
Enzymes for epimerization of isopenicillin N, ring expansion of
penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C.
Function, evolution, refolding, and enzyme engineering.
Ann. N.Y. Acad. Sci. 672 (1992) 396-408. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:9723623]
Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S,
Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J,
Andersson I.
Structure of a cephalosporin synthase.
Nature. 394 (1998) 805-9.
Cephalosporium acremonium |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:2656705]
Dotzlaf JE, Yeh WK.
Purification and properties of deacetoxycephalosporin C synthase
from recombinant Escherichia coli and its comparison with the native
enzyme purified from Streptomyces clavuligerus.
J. Biol. Chem. 264 (1989) 10219-27.
Streptomyces clavuligerus |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.20.1
IUBMB Enzyme Nomenclature: 1.14.20.1
ExPASy - ENZYME nomenclature database: 1.14.20.1
BRENDA, the Enzyme Database: 1.14.20.1
CAS: 85746-10-7 |
|---|
