KEGG   ENZYME: 1.14.21.6Help
Entry
EC 1.14.21.6                Enzyme                                 

Name
lathosterol oxidase;
Delta7-sterol Delta5-dehydrogenase;
Delta7-sterol 5-desaturase;
Delta7-sterol-C5(6)-desaturase;
5-DES
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
5alpha-cholest-7-en-3beta-ol,NAD(P)H:oxygen 5-oxidoreductase
Reaction(IUBMB)
5alpha-cholest-7-en-3beta-ol + NAD(P)H + H+ + O2 = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O [RN:R03310 R07215]
Reaction(KEGG)
Substrate
5alpha-cholest-7-en-3beta-ol [CPD:C01189];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
cholesta-5,7-dien-3beta-ol [CPD:C01164];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This enzyme catalyses the introduction of a C5 double bond into the B ring of Delta7-sterols to yield the corresponding Delta5,7-sterols in mammals, yeast and plants [4]. Forms part of the plant sterol biosynthesis pathway.
History
EC 1.14.21.6 created 1972 as EC 1.3.3.2, transferred 2005 to EC 1.14.21.6
Pathway
Steroid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00227  
lathosterol oxidase
Genes
HSA: 
6309(SC5D)
PTR: 
451624(SC5DL)
PPS: 
100979606(SC5DL)
GGO: 
PON: 
100459805(SC5DL)
MCC: 
706827(SC5DL)
MCF: 
MMU: 
235293(Sc5d)
RNO: 
114100(Sc5d)
CGE: 
100752960(Sc5d)
HGL: 
TUP: 
102481940(SC5D)
CFA: 
610411(SC5D)
AML: 
FCA: 
101099997(SC5D)
PTG: 
102956406(SC5D)
BTA: 
BOM: 
102283021(SC5D)
PHD: 
102340338(SC5D)
CHX: 
102180515(SC5D)
SSC: 
100520925(SC5D)
CFR: 
102520592(SC5D)
BACU: 
103004682(SC5D)
LVE: 
103083016(SC5D)
ECB: 
100063543(SC5D)
MYB: 
102246367(SC5D)
MYD: 
102754805(SC5D)
PALE: 
102891666(SC5D)
MDO: 
100030841(SC5D)
SHR: 
100932949(SC5DL)
OAA: 
100073392(SC5D)
GGA: 
100857756(SC5DL)
MGP: 
TGU: 
FAB: 
101811093(SC5D)
PHI: 
102109366(SC5D)
APLA: 
101803446(SC5D)
FPG: 
101920444(SC5D)
FCH: 
102057522(SC5D)
CLV: 
102095349(SC5D)
ASN: 
102385911(SC5D)
AMJ: 
102571716(SC5D)
PSS: 
102463426(SC5D)
CMY: 
102931181(SC5D)
ACS: 
PBI: 
XLA: 
447026(sc5d)
XTR: 
DRE: 
447891(sc5d)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102365779(SC5D)
CMK: 
103190397(sc5d)
BFO: 
SPU: 
BMOR: 
NVE: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os01t0134500-01(Os01g0134500)
OBR: 
BDI: 
SBI: 
SORBI_03g007010(SORBIDRAFT_03g007010)
ZMA: 
SITA: 
ATR: 
s00079p00182550(AMTR_s00079p00182550)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
MIS: 
MPP: 
BPG: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YLR056W(ERG3)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NDI: 
NDAI_0C06430(NDAI0C06430)
TPF: 
TPHA_0D00440(TPHA0D00440) TPHA_0P00250(TPHA0P00250)
TBL: 
TBLA_0D02520(TBLA0D02520)
TDL: 
TDEL_0C00670(TDEL0C00670)
KAF: 
KAFR_0D01010(KAFR0D01010)
DHA: 
PIC: 
LEL: 
SPAA: 
CAL: 
CaO19.767(ERG3) CaO19.8387(ERG3)
CTP: 
CDU: 
COT: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_42114(AO090701000022)
ANG: 
ANI_1_28134(An15g00150)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
ZTR: 
PFJ: 
BCOM: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
LBC: 
CCI: 
SCM: 
UMA: 
MGL: 
MBR: 
DFA: 
ACAN: 
TET: 
PTM: 
PTI: 
PIF: 
EHX: 
TBR: 
Tb927.8.3240(Tb08.28L1.250)
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
HCH: 
 » show all
Taxonomy
Reference
1  [PMID:14189869]
  Authors
DEMPSEY ME, SEATON JD, SCHROEPFER GJ Jr, TROCKMAN RW.
  Title
THE INTERMEDIARY ROLE OF DELTA-5,7-CHOLESTADIEN-3-BETA-OL IN CHOLESTEROL BIOSYNTHESIS.
  Journal
J. Biol. Chem. 239 (1964) 1381-7.
  Organism
Rattus norvegicus
Reference
2  [PMID:9056262]
  Authors
Nishino H, Nakaya J, Nishi S, Kurosawa T, Ishibashi T.
  Title
Temperature-induced differential kinetic properties between an initial burst and the following steady state in membrane-bound enzymes: studies on lathosterol 5-desaturase.
  Journal
Arch. Biochem. Biophys. 339 (1997) 298-304.
  Organism
Rattus norvegicus
Reference
3  [PMID:8561508]
  Authors
Taton M, Rahier A.
  Title
Plant sterol biosynthesis: identification and characterization of higher plant delta 7-sterol C5(6)-desaturase.
  Journal
Arch. Biochem. Biophys. 325 (1996) 279-88.
  Organism
Zea mays
Reference
4  [PMID:10651635]
  Authors
Taton M, Husselstein T, Benveniste P, Rahier A.
  Title
Role of highly conserved residues in the reaction catalyzed by recombinant Delta7-sterol-C5(6)-desaturase studied by site-directed mutagenesis.
  Journal
Biochemistry. 39 (2000) 701-11.
  Organism
Arabidopsis thaliana
  Sequence
[ath:AT3G02580]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37255-37-1

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