KEGG   ENZYME: 1.3.1.22Help
Entry
EC 1.3.1.22                 Enzyme                                 

Name
3-oxo-5alpha-steroid 4-dehydrogenase (NADP+);
cholestenone 5alpha-reductase;
testosterone Delta4-5alpha-reductase;
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
5alpha-reductase;
steroid 5alpha-hydrogenase;
3-oxosteroid 5alpha-reductase;
testosterone Delta4-hydrogenase;
4-ene-3-oxosteroid 5alpha-reductase;
reduced nicotinamide adenine dinucleotide phosphate:Delta4-3-ketosteroid 5alpha-oxidoreductase;
4-ene-5alpha-reductase;
Delta4-3-ketosteroid 5alpha-oxidoreductase;
cholest-4-en-3-one 5alpha-reductase;
testosterone 5alpha-reductase;
3-oxo-5alpha-steroid 4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3-oxo-5alpha-steroid:NADP+ Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + NADP+ = a 3-oxo-Delta4-steroid + NADPH + H+ [RN:R02610]
Reaction(KEGG)
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
NADP+ [CPD:C00006]
Product
3-oxo-Delta4-steroid [CPD:C00619];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme catalyses the conversion of assorted 3-oxo-Delta4 steroids into their corresponding 5alpha form. Substrates for the mammalian enzyme include testosterone, progesterone, and corticosterone. Substrates for the plant enzyme are brassinosteroids such as campest-4-en-3-one and (22alpha)-hydroxy-campest-4-en-3-one. cf. EC 1.3.99.5, 3-oxo-5alpha-steroid 4-dehydrogenase (acceptor).
History
EC 1.3.1.22 created 1972, modified 2012
Pathway
Steroid hormone biosynthesis
Brassinosteroid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K09591  
steroid 5-alpha-reductase
K12343  
3-oxo-5-alpha-steroid 4-dehydrogenase 1
K12344  
3-oxo-5-alpha-steroid 4-dehydrogenase 2
K12345  
3-oxo-5-alpha-steroid 4-dehydrogenase 3
Genes
HSA: 
6715(SRD5A1) 6716(SRD5A2) 79644(SRD5A3)
PTR: 
100614992(SRD5A2) 471241(SRD5A3) 743740(SRD5A1)
PPS: 
100978730(SRD5A1) 100983163 100986960(SRD5A2) 100987485(SRD5A3)
GGO: 
101127484(SRD5A3) 101138758(SRD5A2) 101143956(SRD5A1)
PON: 
100444341(SRD5A1) 100454013(SRD5A2) 100461714(SRD5A3)
NLE: 
100597634(SRD5A2) 100604898(SRD5A3) 100607932(SRD5A1)
MCC: 
695103(SRD5A1) 696381(SRD5A3) 705676(SRD5A2)
MCF: 
102115639(SRD5A2) 102126671(SRD5A1) 102135568(SRD5A3)
CJC: 
100386366(SRD5A1) 100409457(SRD5A3) 100415292(SRD5A2)
MMU: 
57357(Srd5a3) 78925(Srd5a1) 94224(Srd5a2)
RNO: 
24950(Srd5a1) 305291(Srd5a3) 64677(Srd5a2)
CGE: 
100761447(Srd5a1) 100766917 100770660(Srd5a3)
HGL: 
101708836(Srd5a2) 101714960(Srd5a1) 101726767(Srd5a3)
TUP: 
102478179(SRD5A2) 102493739 102500370(SRD5A1)
CFA: 
403715(SRD5A2) 403716(SRD5A1) 482155(SRD5A3)
AML: 
UMR: 
103671605(SRD5A2) 103672741(SRD5A3) 103679225(SRD5A1)
FCA: 
101086898(SRD5A2) 101090010(SRD5A3) 101098848(SRD5A1)
PTG: 
102951833(SRD5A1) 102953790(SRD5A2) 102960215(SRD5A3)
BTA: 
527024(SRD5A2) 535834(SRD5A3) 614612(SRD5A1)
BOM: 
102271230(SRD5A2) 102273138(SRD5A1) 102284607(SRD5A3)
PHD: 
102326516(SRD5A2) 102328275(SRD5A1) 102331048(SRD5A3)
CHX: 
102172667(SRD5A1) 102180581(SRD5A3) 102187232(SRD5A2)
OAS: 
100642181(SRD5A1) 100820756(SRD5A2) 101106772(SRD5A3)
SSC: 
100518046(SRD5A1) 100525350 397048(ST5AR2)
CFR: 
102514136(SRD5A1) 102523042(SRD5A3) 102523831(SRD5A2)
BACU: 
102998118(SRD5A1) 103005044 103012155(SRD5A3)
LVE: 
103070353(SRD5A1) 103084942(SRD5A2) 103087526(SRD5A3)
ECB: 
100059582(SRD5A3) 100070655(SRD5A2) 100071450(SRD5A1)
MYB: 
102242056(SRD5A2) 102257134(SRD5A3) 102260657(SRD5A1)
MYD: 
102762730(SRD5A1) 102771502(SRD5A3) 102773080(SRD5A2)
PALE: 
102884992(SRD5A2) 102889165(SRD5A1) 102896891(SRD5A3)
MDO: 
100017228(SRD5A3) 100018123(SRD5A1) 100031489(SRD5A2)
SHR: 
100920954(SRD5A1) 100928836(SRD5A3) 100934924
OAA: 
100075423(SRD5A3) 100078235(SRD5A2) 100091321
GGA: 
422750(SRD5A3) 770453(SRD5A1) 772291(SRD5A2)
MGP: 
APLA: 
101792279(SRD5A1) 101796282(SRD5A3) 101799397(SRD5A2)
TGU: 
100224163(SRD5A3) 100225484(SRD5A2) 101233999(SRD5A1)
FAB: 
101808258(SRD5A2) 101812956 101814400(SRD5A1)
PHI: 
102108406(SRD5A2) 102110867(SRD5A1) 102113098(SRD5A3)
FPG: 
101911819(SRD5A1) 101919492(SRD5A2) 101920408(SRD5A3)
FCH: 
102046391(SRD5A2) 102049514(SRD5A1) 102059273(SRD5A3)
CLV: 
102085278(SRD5A2) 102086070(SRD5A1) 102095131(SRD5A3)
ASN: 
102379264 102380496(SRD5A1) 102380562(SRD5A2)
AMJ: 
102565754(SRD5A1) 102567202 102573116(SRD5A2)
PSS: 
102444263(SRD5A3) 102449134(SRD5A1) 102451701(SRD5A2)
CMY: 
102936118(SRD5A2) 102936322(SRD5A3) 102940906(SRD5A1)
ACS: 
100551938(srd5a3) 100555660(srd5a1) 100563846(srd5a2) 103282902
PBI: 
103048475 103063410(SRD5A3) 103065332(SRD5A1)
XLA: 
100049757(srd5a1) 379123(srd5a3)
XTR: 
448586(srd5a1) 549867(srd5a2) 779994(srd5a3)
DRE: 
550388(srd5a2b) 550398(srd5a2a) 560717(srd5a3) 767715(srd5a1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102346093(SRD5A1) 102355026 102363132(SRD5A3)
CMK: 
103178169(srd5a3) 103181748(srd5a2) 103183194(srd5a1)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CBR: 
BMY: 
LOA: 
HRO: 
LGI: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
AT2G38050(DET2)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0004s17050g(POPTRDRAFT_759753) POPTR_0005s04930g POPTR_0009s12810g(POPTRDRAFT_557164) POPTR_0013s13810g(POPTRDRAFT_774012) POPTR_0016s11800g(POPTRDRAFT_576916)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os01t0851600-01(Os01g0851600) Os04t0576800-01(Os04g0576800) Os07t0493400-01(Os07g0493400) Os11t0184100-01(Os11g0184100)
OBR: 
BDI: 
SBI: 
SORBI_03g040050(SORBIDRAFT_03g040050)
ZMA: 
SITA: 
PDA: 
ATR: 
s00029p00205400(AMTR_s00029p00205400) s00182p00010830(AMTR_s00182p00010830)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
SCE: 
YIL049W(DFG10)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0A13460(NCAS0A13460)
NDI: 
NDAI_0A02580(NDAI0A02580)
TPF: 
TPHA_0K00940(TPHA0K00940)
TBL: 
TBLA_0J01440(TBLA0J01440)
TDL: 
TDEL_0H02090(TDEL0H02090)
KAF: 
KAFR_0I01850(KAFR0I01850)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.209(DFG10) CaO19.7841(DFG10)
CTP: 
COT: 
CDU: 
CD36_23150(DFG10)
CTEN: 
YLI: 
CLU: 
NCR: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_1468144(AO090023000838) AOR_1_652184(AO090009000397)
ANG: 
ANI_1_570164(An18g04290)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
TMS: 
PPL: 
DSQ: 
SHS: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT59271(AGABI1DRAFT_59271)
ABV: 
AGABI2DRAFT224531(AGABI2DRAFT_224531)
CPUT: 
SLA: 
UMA: 
PFP: 
MGL: 
MLR: 
WSE: 
MBR: 
DPP: 
DFA: 
EHI: 
EHI_197070(92.t00023)
EDI: 
ACAN: 
PFA: 
PFH: 
PKN: 
PVX: 
PCY: 
TGO: 
TET: 
PTM: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
TBR: 
Tb927.8.2480(Tb08.26A17.360)
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
TVA: 
GPB: 
LLA: 
L176238(yfhH)
MUL: 
MAB: 
MABB: 
MAK: 
MAZ: 
MJD: 
MMI: 
MLI: 
NFA: 
REQ: 
ACTN: 
TDE: 
TPED: 
TPK: 
STA: 
STQ: 
BTH: 
BFR: 
BFS: 
BFG: 
BVU: 
BSA: 
BXY: 
BDO: 
BDH: 
BVS: 
AFD: 
ASH: 
FTE: 
 » show all
Taxonomy
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J. Biol. Chem. 234 (1959) 2014-21.
  Sequence
Reference
2  [PMID:5907469]
  Authors
Shefer S, Hauser S, Mosbach EH.
  Title
Studies on the biosynthesis of 5-alpha-cholestan-3-beta-ol. I. Cholestenone 5-alpha-reductase of rat liver.
  Journal
J. Biol. Chem. 241 (1966) 946-52.
Reference
3  [PMID:1166484]
  Authors
Cheng YJ, Karavolas HJ.
  Title
Properties and subcellular distribution of delta4-steroid (progesterone) 5alpha-reductase in rat anterior pituitary.
  Journal
Steroids. 26 (1975) 57-71.
Reference
4  [PMID:1705142]
  Authors
Sargent NS, Habib FK
  Title
Partial purification of human prostatic 5 alpha-reductase (3-oxo-5 alpha-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form.
  Journal
J. Steroid. Biochem. Mol. Biol. 38 (1991) 73-7.
Reference
5  [PMID:7900170]
  Authors
Quemener E, Amet Y, di Stefano S, Fournier G, Floch HH, Abalain JH
  Title
Purification of testosterone 5 alpha-reductase from human prostate by a four-step chromatographic procedure.
  Journal
Steroids. 59 (1994) 712-8.
Reference
6  [PMID:9202401]
  Authors
Poletti A, Celotti F, Rumio C, Rabuffetti M, Martini L
  Title
Identification of type 1 5alpha-reductase in myelin membranes of male and female  rat brain.
  Journal
Mol. Cell. Endocrinol. 129 (1997) 181-90.
Reference
7  [PMID:9108014]
  Authors
Li J, Biswas MG, Chao A, Russell DW, Chory J
  Title
Conservation of function between mammalian and plant steroid 5alpha-reductases.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 3554-9.
  Sequence
[ath:AT2G38050]
Reference
8  [PMID:15993049]
  Authors
Rosati F, Bardazzi I, De Blasi P, Simi L, Scarpi D, Guarna A, Serio M, Racchi ML, Danza G.
  Title
5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes.
  Journal
J. Steroid. Biochem. Mol. Biol. 96 (2005) 287-99.
  Sequence
[sly:543801]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37255-34-8

DBGET integrated database retrieval system