| Entry |
|
|---|
| Name |
Delta4-3-oxosteroid 5beta-reductase;
3-oxo-Delta4-steroid 5beta-reductase;
5beta-reductase;
androstenedione 5beta-reductase;
cholestenone 5beta-reductase;
cortisone 5beta-reductase;
cortisone beta-reductase;
cortisone Delta4-5beta-reductase;
steroid 5beta-reductase;
testosterone 5beta-reductase;
Delta4-3-ketosteroid 5beta-reductase;
Delta4-5beta-reductase;
Delta4-hydrogenase;
4,5beta-dihydrocortisone:NADP+ Delta4-oxidoreductase;
3-oxo-5beta-steroid:NADP+ Delta4-oxidoreductase |
|---|
| Class |
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
 |
|---|
| Sysname |
5beta-cholestan-3-one:NADP+ 4,5-oxidoreductase |
|---|
| Reaction(IUBMB) |
(1) 5beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH + H+
[RN:R02609];
(2) 17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ =
cortisone + NADPH + H+ [RN:R02893] |
|---|
| Reaction(KEGG) |
R02609 R02893;
(other) R01835 R02219 R02498 R02841 R03713 R03850 R04817 R04823
 |
|---|
| Substrate |
5beta-cholestan-3-one [CPD:C03091];
NADP+ [CPD:C00006];
17,21-dihydroxy-5beta-pregnane-3,11,20-trione |
|---|
| Product |
cholest-4-en-3-one [CPD:C00599];
NADPH [CPD:C00005];
H+ [CPD:C00080];
cortisone [CPD:C00762] |
|---|
| Comment |
The enzyme from human efficiently catalyses the reduction of
progesterone, androstenedione, 17alpha-hydroxyprogesterone and
testosterone to 5beta-reduced metabolites; it can also act on
aldosterone, corticosterone and cortisol, but to a lesser extent
[8]. The bile acid intermediates
7alpha,12alpha-dihydroxy-4-cholesten-3-one and
7alpha-hydroxy-4-cholesten-3-one can also act as substrates [9]. |
|---|
| Pathway |
PATH: ec00120 Primary bile acid biosynthesis
PATH: ec00140 Steroid hormone biosynthesis
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K00251 3-oxo-5-beta-steroid 4-dehydrogenase |
|---|
| Genes |
HSA: 6718(AKR1D1)
PTR: 472607(AKR1D1)
MCC: 709863(AKR1D1)
MMU: 208665(Akr1d1)
RNO: 192242(Akr1d1)
CFA: 482711(AKR1D1)
BTA: 513855(AKR1D1)
ECB: 100066919
MDO: 100022753
GGA: 418107(AKR1D1)
TGU: 100228769
XLA: 399023
XTR: 594997
 |
|---|
| Structures |
PDB: 3BUR 3BUV 3BV7 3CAQ 3CAS 3CAV 3CMF 3COT 3DOP 3G1R |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:13376613]
DORFMAN RI, FORCHIELLI E.
Separation of delta 4-5 alpha-hydrogenases from rat liver
homogenates.
J. Biol. Chem. 223 (1956) 443-8.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:13805063]
BROWN-GRANT K, FORCHIELLI E, DORFMAN RI.
The delta4-hydrogenases of guinea pig adrenal gland.
J. Biol. Chem. 235 (1960) 1317-20.
Cavia porcellus |
|---|
Reference
Authors
Title
Journal
|
3
Levy, H.R. and Talalay, P.
Enzymatic introduction of double bonds into steroid ring A.
J. Am. Chem. Soc. 79 (1957) 2658-2659. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:13416214]
TOMKINS GM.
The enzymatic reduction of delta 4-3-ketosteroids.
J. Biol. Chem. 225 (1957) 13-24.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:2278855]
Sugimoto Y, Yoshida M, Tamaoki B.
Purification of 5 beta-reductase from hepatic cytosol fraction of
chicken.
J. Steroid. Biochem. Mol. Biol. 37 (1990) 717-24.
Gallus gallus [GN:gga] |
|---|
Reference
Authors
Title
Journal
Organism
|
6 [PMID:3828348]
Furuebisu M, Deguchi S, Okuda K.
Identification of cortisone 5 beta-reductase as delta
4-3-ketosteroid 5 beta-reductase.
Biochim. Biophys. Acta. 912 (1987) 110-4.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
7 [PMID:6736016]
Okuda A, Okuda K.
Purification and characterization of delta 4-3-ketosteroid 5
beta-reductase.
J. Biol. Chem. 259 (1984) 7519-24.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
8 [PMID:11342103]
Charbonneau A, The VL.
Genomic organization of a human 5beta-reductase and its pseudogene
and substrate selectivity of the expressed enzyme.
Biochim. Biophys. Acta. 1517 (2001) 228-35.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
9 [PMID:7508385]
Kondo KH, Kai MH, Setoguchi Y, Eggertsen G, Sjoblom P, Setoguchi T,
Okuda KI, Bjorkhem I.
Cloning and expression of cDNA of human delta 4-3-oxosteroid 5
beta-reductase and substrate specificity of the expressed enzyme.
Eur. J. Biochem. 219 (1994) 357-63.
Homo sapiens [GN:hsa] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.3.1.3
IUBMB Enzyme Nomenclature: 1.3.1.3
ExPASy - ENZYME nomenclature database: 1.3.1.3
BRENDA, the Enzyme Database: 1.3.1.3
CAS: 9029-08-7 |
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