| Entry |
|
|---|
| Name |
precorrin-2 dehydrogenase;
Met8p;
SirC;
CysG |
|---|
| Class |
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
 |
|---|
| Sysname |
precorrin-2:NAD+ oxidoreductase |
|---|
| Reaction(IUBMB) |
precorrin-2 + NAD+ = sirohydrochlorin + NADH + H+ [RN:R03947] |
|---|
| Reaction(KEGG) |
R03947
 |
|---|
| Substrate |
precorrin 2 [CPD:C02463];
NAD+ [CPD:C00003] |
|---|
| Product |
sirohydrochlorin [CPD:C05778];
NADH [CPD:C00004];
H+ [CPD:C00080] |
|---|
| Comment |
This enzyme catalyses the second of three steps leading to the
formation of siroheme from uroporphyrinogen III. The first step
involves the donation of two S-adenosyl-L-methionine-derived methyl
groups to carbons 2 and 7 of uroporphyrinogen III to form
precorrin-2 (EC 2.1.1.107, uroporphyrin-III C-methyltransferase) and
the third step involves the chelation of ferrous iron to
sirohydrochlorin to form siroheme (EC 4.99.1.4, sirohydrochlorin
ferrochelatase). In Saccharomyces cerevisiae, the last two steps are
carried out by a single bifunctional enzyme, Met8p. In some
bacteria, steps 1-3 are catalysed by a single multifunctional
protein called CysG, whereas in Bacillus megaterium, three separate
enzymes carry out each of the steps, with SirC being responsible for
the above reaction. |
|---|
| Pathway |
PATH: ec00860 Porphyrin and chlorophyll metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K02302 uroporphyrin-III C-methyltransferase / precorrin-2
dehydrogenase / sirohydrochlorin ferrochelatase
KO: K02304 precorrin-2 dehydrogenase / sirohydrochlorin
ferrochelatase |
|---|
| Genes |
SCE: YBR213W(MET8)
AGO: AGOS_AAR152W
KLA: KLLA0C05544g
DHA: DEHA0F03047g
PIC: PICST_63116(MET8)
VPO: Kpol_530p45
CAL: CaO19.14072(MET8) CaO19.6780(MET8)
CGR: CAGL0K06677g
YLI: YALI0E11385g
SPO: SPAC4D7.06c
NCR: NCU07585
PAN: PODANSg303
MGR: MGG_09871
FGR: FG04222.1
ANI: AN6710.2
AFM: AFUA_7G05680
AOR: AO090005000430
ANG: An07g02200
NFI: NFIA_026930
SSL: SS1G_14076
BFU: BC1G_13689
CNE: CNF00750
CNB: CNBF3830
LBC: LACBIDRAFT_146719
UMA: UM00458.1
MGL: MGL_2195
ECO: b3368(cysG)
ECJ: JW3331(cysG)
ECD: ECDH10B_3544(cysG)
EBW: BWG_3060(cysG)
ECE: Z4729(cysG)
ECS: ECs4219(cysG)
ECF: ECH74115_4679(cysG)
ETW: ECSP_4326(cysG)
ECG: E2348C_3618(cysG)
ECC: c4144(cysG)
ECI: UTI89_C3872(cysG)
ECP: ECP_3459(cysG)
ECV: APECO1_3088(cysG)
ECW: EcE24377A_3838(cysG)
ECX: EcHS_A3564(cysG)
ECM: EcSMS35_3650(cysG)
ECY: ECSE_3630(cysG)
ECL: EcolC_0344(cysG)
ECK: EC55989_3774(cysG)
ECQ: ECED1_4032(cysG)
ECR: ECIAI1_3507(cysG)
ECT: ECIAI39_3852(cysG)
ECZ: ECS88_3759(cysG)
EUM: ECUMN_3831(cysG)
ELF: LF82_0414(cysG)
EBL: B21_03171(cysG)
EBD: ECBD_0380
EBR: ECB_03219(cysG)
EOH: ECO103_4087(cysG)
EOI: ECO111_4178(cysG)
EOJ: ECO26_4457(cysG)
EFE: EFER_3340(cysG)
STY: STY4319(cysG)
STT: t4028(cysG)
SPT: SPA3343(cysG)
SEK: SSPA3121(cysG)
SPQ: SPAB_04328(cysG)
SEI: SPC_3547(cysG)
SEC: SC3411(cysG)
SEH: SeHA_C3784(cysG)
SEE: SNSL254_A3749(cysG)
SEW: SeSA_A3675(cysG)
SEA: SeAg_B3675(cysG)
SED: SeD_A3846(cysG)
SEG: SG3962(cysG)
SET: SEN3304(cysG)
SES: SARI_04135(cysG)
STM: STM3477(cysG)
YPE: YPO0158(cysG) YPO3367(cysG)
YPK: y3941(cysG)
YPM: YP_0160(cysG)
YPA: YPA_2776 YPA_3311(cysG)
YPN: YPN_0726 YPN_3907(cysG)
YPG: YpAngola_A0970(cysG2) YpAngola_A3719(cysG)
YPP: YPDSF_0085(cysG) YPDSF_2993
YPS: YPTB0764(cysG) YPTB3743(cysG)
YPI: YpsIP31758_3305(cysG2) YpsIP31758_3959(cysG)
YPY: YPK_0232(cysG) YPK_3437
YPB: YPTS_0798 YPTS_3938(cysG)
YEN: YE0761(cysG) YE3968(cysG)
SFL: SF3387(cysG)
SFX: S4376(cysG)
SFV: SFV_3374(cysG)
SSN: SSON_3499(cysG)
SBO: SBO_3350(cysG)
SBC: SbBS512_E3744(cysG)
SDY: SDY_3530(cysG)
ECA: ECA3544(cysG1) ECA4081(cysG)
PCT: PC1_2728 PC1_3362 PC1_3871
PWA: Pecwa_1546 Pecwa_3519 Pecwa_4048
ETA: ETA_27070(cysG)
PLU: plu0708(cysG)
PAY: PAU_00681(cysG)
BUC: BU425(cysG)
BAP: BUAP5A_418(cysG)
BAU: BUAPTUC7_419(cysG)
SGL: SG0520
ENT: Ent638_3796(cysG)
ESA: ESA_00538 ESA_04366(cysG)
CTU: Ctu_33260(cysG1) Ctu_38860(cysG2)
KPN: KPN_03115(cysG) KPN_03754(cysG)
KPE: KPK_0362(cysG) KPK_1008(cysF)
KPU: KP1_4385(cysG) KP1_5084(cysG)
CKO: CKO_04792(cysG)
SPE: Spro_0817 Spro_2904 Spro_4598(cysG)
PMR: PMI1477(cysG) PMI2247(cysG)
EIC: NT01EI_3617
ETR: ETAE_3254(cysG)
BFL: Bfl161(cysG)
BPN: BPEN_166(cysG)
DDA: Dd703_0861 Dd703_2492
DDC: Dd586_2701 Dd586_3313
DZE: Dd1591_0830 Dd1591_1443
MSU: MS1254(cysG)
APJ: APJL_1886(cysG)
APA: APP7_1934
ASU: Asuc_1691
XFA: XF0832
XFT: PD1840(cysG)
XFM: Xfasm12_2018
XFN: XfasM23_1941
XCC: XCC2003(cysG) XCC3181(cysG)
XCB: XC_0983 XC_2181
XCA: xccb100_0995(cysG) xccb100_2303
XCV: XCV2089 XCV3458(cysG)
XAC: XAC2159(cysG) XAC3340(cysG)
XOO: XOO3102(cysG) XOO3408(cysG)
XOM: XOO_2947 XOO_3208
XOP: PXO_01486 PXO_02124
VCH: VC1363
VCO: VC0395_A0976
VCM: VCM66_2481(cysG)
VCJ: VCD_001802
VVU: VV1_2702
VVY: VV1558
VPA: VP1619
VHA: VIBHAR_00765
VSP: VS_II0671
VEX: VEA_000072 VEA_001708
VFI: VF_1531
VFM: VFMJ11_0809(cobA)
VSA: VSAL_I0795(cysG)
PPR: PBPRA1425 PBPRA2524 PBPRA3312 PBPRB0870
PAE: PA2611(cysG)
PAU: PA14_30340(cysG)
PAP: PSPA7_2597(cysG)
PAG: PLES_24941(cysG)
PPU: PP_3999(cobA-2)
PPF: Pput_1834
PPG: PputGB1_3604
PPW: PputW619_3403
PST: PSPTO_3344(cysG)
PSB: Psyr_3174
PSP: PSPPH_3088(cysG)
PFL: PFL_3875
PFO: Pfl01_3581
PFS: PFLU3796
PEN: PSEEN2217(cysG)
PMY: Pmen_2383
PSA: PST_2288(cysG)
CJA: CJA_0323 CJA_2551
AVN: Avin_28170(cobA)
PAR: Psyc_1065(cysG)
PCR: Pcryo_1397
PRW: PsycPRwf_1107
ACI: ACIAD2934(cysG)
ACB: A1S_2697
ABM: ABSDF0748(cysG)
ABY: ABAYE0758(cysG)
ABC: ACICU_02978
ABN: AB57_3146
ABB: ABBFA_000742(cobA)
SON: SO_3108
SDN: Sden_3717
SAZ: Sama_2187
SHN: Shewana3_1441
SHW: Sputw3181_1529
PHA: PSHAa0213(cysG)
PAT: Patl_0417
SDE: Sde_1698
MAQ: Maqu_1546
AMC: MADE_03694
PIN: Ping_0298
TTU: TERTU_1974(cysG) TERTU_2949(cobA)
MCA: MCA2089(cysG)
TCX: Tcr_1160
NOC: Noc_0863
AEH: Mlg_1679
HHA: Hhal_1343 Hhal_1933
TGR: Tgr7_2173
HNA: Hneap_1111
HCH: HCH_02450(cysG)
CSA: Csal_2314
ABO: ABO_1295(cysG)
MMW: Mmwyl1_3251
AHA: AHA_2578 AHA_3568 AHA_4121
ASA: ASA_0192(cysG) ASA_3524(cysG)
TAU: Tola_2546
BCI: BCI_0215(cysG)
RMA: Rmag_0270
VOK: COSY_0254(cysG)
KKO: Kkor_0729
NMA: NMA1367
NME: NMB1156(cysG-1) NMB1194(cysG-2)
NMC: NMC1096(cysG)
NMN: NMCC_1074(cysG)
NMI: NMO_1012(cysG)
CVI: CV_0813(cobA2)
LHK: LHK_02945(cobA2)
BPE: BP1055(cysG)
BPA: BPP1151(cysG)
BBR: BB1367(cysG)
BPT: Bpet3643(cysG1)
BAV: BAV0848(cysG)
POL: Bpro_2765
AJS: Ajs_1913
DIA: Dtpsy_1718
MPT: Mpe_A1498
LCH: Lcho_1441
NET: Neut_1002
NMU: Nmul_A2313
TBD: Tbd_2471
MFA: Mfla_0652
MMB: Mmol_1864
MEI: Msip34_2114
APP: CAP2UW1_2490
WSU: WS1003(cysG)
GLO: Glov_3656
DVU: DVU1463
DVL: Dvul_1616
DDE: Dde_2023
LIP: LI0320(cysG)
ADE: Adeh_3154
ACP: A2cp1_3345
AFW: Anae109_3603
ANK: AnaeK_3267
SAT: SYN_02276
SFU: Sfum_1588
MLO: mll3232
MES: Meso_3989
SME: SMc01053(cysG)
SMD: Smed_1099 Smed_4522
ATU: Atu1454(cysG) Atu3899(cysG)
ATC: AGR_C_2683 AGR_L_1897
ARA: Arad_2277(cysG)
AVI: Avi_2146(cysG) Avi_5891(cysG)
RET: RHE_CH01958(cysG)
REC: RHECIAT_CH0002071(cysG)
RLE: RL2288(cysG2)
RLT: Rleg2_1636
RLG: Rleg_1823
RHI: NGR_b11360 NGR_c13050(cysG)
BME: BMEI1768
BMF: BAB1_0179(cysG)
BMB: BruAb1_0175(cysG)
BMC: BAbS19_I01700
BMS: BR0179(cysG)
BMT: BSUIS_A0180(cobA)
BOV: BOV_0173(cysG)
BCS: BCAN_A0184(cobA)
BMR: BMI_I182(cysG)
OAN: Oant_0188
BJA: blr1477
BRA: BRADO1065(cysG)
BBT: BBta_6982(cysG)
RPA: RPA4215(cysG)
RPB: RPB_1399
RPC: RPC_4015
RPD: RPD_1379
RPE: RPE_1763
RPT: Rpal_4696
NWI: Nwi_2762
NHA: Nham_3562
OCA: OCAR_6833 OCAR_7388
XAU: Xaut_1077 Xaut_2375
AZC: AZC_0234
MEX: Mext_3223
MEA: Mex_1p3434(cysG)
MDI: METDI4011(cysG)
MRD: Mrad2831_2296
MET: M446_3750
MPO: Mpop_3418
MCH: Mchl_3547
MNO: Mnod_2864
BID: Bind_0267
MSL: Msil_3024
CCR: CC_0024
CCS: CCNA_01175
CAK: Caul_3269
RSK: RSKD131_0256
JAN: Jann_1775 Jann_3047
RDE: RD1_2964(cysG) RD1_4164(cysG)
DSH: Dshi_1155 Dshi_1671(cysG)
MMR: Mmar10_0347
HNE: HNE_0322(cysG)
HBA: Hbal_0501 Hbal_2778
ZMO: ZMO0006 ZMO1271
ZMN: Za10_0069 Za10_1171
SWI: Swit_4060
GOX: GOX2065
GBE: GbCGDNIH1_0735
GDI: GDI_0165(cysG)
GDJ: Gdia_2235
APT: APA01_21940
RRU: Rru_A1933
RCE: RC1_2177(cysG)
BSU: BSU15630(sirC)
BHA: BH1497
BAN: BA1447 BA2142
BAR: GBAA1447 GBAA2142
BAA: BA_1968 BA_2638
BAT: BAS1337 BAS1993
BCE: BC1428 BC2132
BCA: BCE_1551
BCZ: BCZK1311(cysG) BCZK1945
BTK: BT9727_1310(cysG) BT9727_1966
BTL: BALH_1283 BALH_1903
BLI: BL02289(sirA)
BLD: BLi01784(ylnF)
OIH: OB1657
GKA: GK0404
SAU: SA2412
SAV: SAV2619
SAM: MW2539
SAR: SAR2697
SAS: SAS2505
SAC: SACOL2638
SAB: SAB2493c
SAA: SAUSA300_2553
SAO: SAOUHSC_02945
SAJ: SaurJH9_2642
SAH: SaurJH1_2697
SAE: NWMN_2517(cysG)
SEP: SE2177
SER: SERP2188
SHA: SH0417
SSP: SSP2404
LMO: lmo1141
LMF: LMOf2365_1148
LIN: lin1105
LWE: lwe1099
SSA: SSA_0483
LRE: Lreu_1704
CAC: CAC0096(hemW)
CPE: CPE1436
CPR: CPR_1423
CTC: CTC00726
DSY: DSY2227(cysG)
EEL: EUBELI_00453
CSC: Csac_1650
MTU: Rv2847c(cysG)
MTC: MT2913(cysG)
MRA: MRA_2870(cysG)
MTF: TBFG_12862
MTB: TBMG_01125(TBMG_01125.1)
MBO: Mb2872c(cysG)
MBB: BCG_2867c(cysG)
MBT: JTY_2862(cysG)
MPA: MAP2916c(cysG2)
MAV: MAV_3703(cobA)
MSM: MSMEG_2618(cobA)
MUL: MUL_2114(cysG)
MVA: Mvan_2305
MGI: Mflv_4047
MAB: MAB_3143c
MMC: Mmcs_2074
MKM: Mkms_2120
MJL: Mjls_2057
MMI: MMAR_1886(cysG)
NFA: nfa40770(cysG)
RHA: RHA1_ro06617
RER: RER_26100(cysG)
ROP: ROP_66530(cysG)
GBR: Gbro_2143
SCO: SCO1553(SCL11.09c)
SMA: SAV_6796(cysG)
SGR: SGR_5984
ART: Arth_3111
AAU: AAur_3076(cobA)
ACH: Achl_2812
RSA: RSal33209_2609
KRH: KRH_11210(cysG)
MLU: Mlut_10020
BCV: Bcav_2797
JDE: Jden_0658
XCE: Xcel_0711
NCA: Noca_2600
TFU: Tfu_2221
TCU: Tcur_1323
SRO: Sros_7364
ACE: Acel_1616
NML: Namu_0162 Namu_2248
KRA: Krad_1326
SEN: SACE_5957
SVI: Svir_22640
AMI: Amir_5854
STP: Strop_3545
SAQ: Sare_3920
CAI: Caci_2321
LIL: LA4217
LIC: LIC13368(cysG)
LBJ: LBJ_2838(cysG)
LBL: LBL_0233(cysG)
LBI: LEPBI_I2768(cobA2)
LBF: LBF_2683
BBL: BLBBGE_278(sirA)
BPI: BPLAN_360(cysG)
FNU: FN0539
OTE: Oter_2433
NPU: Npun_R1873
CTE: CT2239
CCH: Cag_1943
CPH: Cpha266_0141
PVI: Cvib_0052
PLT: Plut_0049
RRS: RoseRS_0573
RCA: Rcas_0636
CAU: Caur_0688 Caur_2564
CAG: Cagg_1263
CHL: Chy400_2771
TTH: TTC0311
TTJ: TTHA0670
AAE: aq_1237(cysG)
MJA: MJ0140
MMP: MMP0089
MMQ: MmarC5_1587
MAC: MA0576(cysG)
MBA: Mbar_A1461
MMA: MM_1740
MBU: Mbur_1230
MTH: MTH1013
MKA: MK1495(cysG_1)
AFU: AF1592
HAL: VNG1775C
HSL: OE3498R(cysG1)
HMA: rrnAC1709(hemX)
HWA: HQ3335A(cysG)
NPH: NP4500A(cysG1)
HLA: Hlac_2131
TAC: Ta0652
TVO: TVN0924
RCI: RCIX909
APE: APE_1491.1(sirC)
STO: ST0211
SAI: Saci_0776
SIS: LS215_2119
SIA: M1425_1956
SIM: M1627_2034
SID: M164_1964
SIY: YG5714_2078
SIN: YN1551_0840
MSE: Msed_0213
PAI: PAE0585
PIS: Pisl_0119
PCL: Pcal_1710
PAS: Pars_2248
TNE: Tneu_1896
 |
|---|
| Structures |
PDB: 3DFZ |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:11980703]
Schubert HL, Raux E, Brindley AA, Leech HK, Wilson KS, Hill CP,
Warren MJ.
The structure of Saccharomyces cerevisiae Met8p, a bifunctional
dehydrogenase and ferrochelatase.
EMBO. J. 21 (2002) 2068-75.
Saccharomyces cerevisiae [GN:sce] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:12195810]
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
The biosynthesis of adenosylcobalamin (vitamin B12).
Nat. Prod. Rep. 19 (2002) 390-412.
Saccharomyces cerevisiae [GN:sce], Salmonella enterica |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.3.1.76
IUBMB Enzyme Nomenclature: 1.3.1.76
ExPASy - ENZYME nomenclature database: 1.3.1.76
BRENDA, the Enzyme Database: 1.3.1.76
CAS: 227184-47-6 |
|---|
