| Entry |
|
|---|
| Name |
cytokinin dehydrogenase;
N6-dimethylallyladenine:(acceptor) oxidoreductase;
6-N-dimethylallyladenine:acceptor oxidoreductase;
OsCKX2;
CKX;
cytokinin oxidase/dehydrogenase |
|---|
| Class |
Oxidoreductases;
Acting on the CH-NH group of donors;
With other acceptors
 |
|---|
| Sysname |
N6-dimethylallyladenine:acceptor oxidoreductase |
|---|
| Reaction(IUBMB) |
N6-dimethylallyladenine + acceptor + H2O = adenine +
3-methylbut-2-enal + reduced acceptor [RN:R05708] |
|---|
| Reaction(KEGG) |
R05708
 |
|---|
| Substrate |
N6-dimethylallyladenine [CPD:C04083];
acceptor [CPD:C00028];
H2O [CPD:C00001] |
|---|
| Product |
adenine [CPD:C00147];
3-methylbut-2-enal [CPD:C07330];
reduced acceptor [CPD:C00030] |
|---|
| Comment |
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family
of N6-substituted adenine derivatives that are plant hormones, where
the substituent is a dimethylallyl or other prenyl group. Although
this activity was previously thought to be catalysed by a
hydrogen-peroxide-forming oxidase, this enzyme does not require
oxygen for activity and does not form hydrogen peroxide.
2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium,
phenazine methosulfate and Cu(II) in the presence of imidazole can
act as acceptors. This enzyme plays a part in regulating rice-grain
production, with lower levels of the enzyme resulting in enhanced
grain production [2]. |
|---|
| Pathway |
ec00908 Zeatin biosynthesis |
|---|
| Orthology |
K00279 cytokinin dehydrogenase |
|---|
| Genes |
ATH: AT1G75450(CKX5) AT2G19500(CKX2) AT2G41510(CKX1) AT4G29740(CKX4)
AT5G21482(CKX7) AT5G56970(CKX3)
OSA: 4338605(Os05g0374200)
ANA: all0324
AVA: Ava_4717
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:11168382]
Galuszka P, Frebort I, Sebela M, Sauer P, Jacobsen S, Pec P.
Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin
degradation in cereals.
Eur. J. Biochem. 268 (2001) 450-61.
Arabidopsis thaliana [GN:ath] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:15976269]
Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A,
Angeles ER, Qian Q, Kitano H, Matsuoka M
Cytokinin oxidase regulates rice grain production.
Science. 309 (2005) 741-5.
Oryza sativa |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.5.99.12
IUBMB Enzyme Nomenclature: 1.5.99.12
ExPASy - ENZYME nomenclature database: 1.5.99.12
BRENDA, the Enzyme Database: 1.5.99.12
CAS: 55326-39-1 |
|---|
