KEGG   ENZYME: 1.6.3.1Help
Entry
EC 1.6.3.1                  Enzyme                                 

Name
NAD(P)H oxidase (H2O2-forming);
THOX2;
ThOX;
dual oxidase;
p138tox;
thyroid NADPH oxidase;
thyroid oxidase;
thyroid oxidase 2;
NADPH oxidase;
NAD(P)H:oxygen oxidoreductase;
NAD(P)H oxidase
Class
Oxidoreductases;
Acting on NADH or NADPH;
With oxygen as acceptor
BRITE hierarchy
Sysname
NAD(P)H:oxygen oxidoreductase (H2O2-forming)
Reaction(IUBMB)
NAD(P)H + H+ + O2 = NAD(P)+ + H2O2 [RN:R07171 R07172]
Reaction(KEGG)
Substrate
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O2 [CPD:C00027]
Comment
Requires FAD, heme and calcium. When calcium is present, this transmembrane glycoprotein generates H2O2 by transfering electrons from intracellular NAD(P)H to extracellular molecular oxygen. The electron bridge within the enzyme contains one molecule of FAD and probably two heme groups. This flavoprotein is expressed at the apical membrane of thyrocytes, and provides H2O2 for the thyroid peroxidase-catalysed biosynthesis of thyroid hormones.
History
EC 1.6.3.1 created 2003, modified 2013
Orthology
K13411  
dual oxidase
Genes
HSA: 
50506(DUOX2) 53905(DUOX1)
PTR: 
453395(DUOX2) 467673(DUOX1)
PPS: 
100974723(DUOX1) 100978429(DUOX2)
GGO: 
101137548(DUOX1) 101139037(DUOX2)
PON: 
100445893(DUOX2) 100447745(DUOX1)
NLE: 
100588618(DUOX1) 100595406(DUOX2)
MCC: 
713126(DUOX1) 713906
MCF: 
102131806(DUOX2) 102133834(DUOX1)
CJC: 
100387872(DUOX2) 100406741(DUOX1)
MMU: 
214593(Duox2) 99439(Duox1)
RNO: 
266807(Duox1) 79107(Duox2)
CGE: 
NGI: 
103746898(Duox2) 103746936(Duox1)
HGL: 
101698133(Duox1) 101698889(Duox2)
OCU: 
100344845(DUOX1) 100345610(DUOX2)
TUP: 
102471164(DUOX2) 102496442(DUOX1)
CFA: 
403719(DUOX2) 403720(DUOX1)
UMR: 
103656587(DUOX2) 103656588(DUOX1)
FCA: 
101087408(DUOX2) 101091374(DUOX1)
PTG: 
102968075(DUOX2) 102968369(DUOX1)
BTA: 
510409(DUOX1) 785698(DUOX2)
BOM: 
102272102(DUOX2) 102277170(DUOX1)
PHD: 
102333965(DUOX1) 102334675(DUOX2)
CHX: 
102179569(DUOX1) 102179857(DUOX2)
OAS: 
SSC: 
397060(DUOX2) 397177(DUOX1)
CFR: 
102510558(DUOX1) 102510795(DUOX2)
BACU: 
103001055(DUOX2) 103001339(DUOX1)
LVE: 
103071982(DUOX2) 103076766(DUOX1)
ECB: 
100070541(DUOX1) 100070566(DUOX2)
MYB: 
102242003(DUOX2) 102252627(DUOX1)
MYD: 
102771512(DUOX1) 102771797(DUOX2)
PALE: 
102894515(DUOX1) 102895435(DUOX2)
MDO: 
100012658(DUOX2) 100012836(DUOX1)
SHR: 
100931267(DUOX2) 100932050(DUOX1)
OAA: 
100089154(DUOX2)
GGA: 
427482(DUOX2)
MGP: 
APLA: 
101800626(DUOX2)
TGU: 
100218721(DUOX2)
FAB: 
101805677(DUOX2)
PHI: 
102105296(DUOX2)
FPG: 
101914675(DUOX2)
FCH: 
102054788(DUOX2)
CLV: 
102097710(DUOX2)
ASN: 
102372872(DUOX2)
AMJ: 
102573689(DUOX2)
PSS: 
102461565(DUOX2)
CMY: 
102933380(DUOX2)
ACS: 
PBI: 
103059551(DUOX2)
XTR: 
100486050(duox2) 100486194(duox1)
DRE: 
565097(duox)
TRU: 
100124426(duox)
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
BFO: 
CIN: 
100170036(duox-d) 100170037(duox-c) 100170038(duox-b) 100170039(duox-a)
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
551970(Duox)
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CBR: 
CBG05103(Cbr-bli-3)
BMY: 
LOA: 
TSP: 
LGI: 
AQU: 
TCC: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
POP: 
SOT: 
SBI: 
SORBI_03g039050(SORBIDRAFT_03g039050)
ATR: 
s00023p00181580(AMTR_s00023p00181580) s00066p00037020(AMTR_s00066p00037020)
 » show all
Taxonomy
Reference
1  [PMID:12110737]
  Authors
Moreno JC, Bikker H, Kempers MJ, van Trotsenburg AS, Baas F, de Vijlder JJ, Vulsma T, Ris-Stalpers C.
  Title
Inactivating mutations in the gene for thyroid oxidase 2 (THOX2) and congenital hypothyroidism.
  Journal
N. Engl. J. Med. 347 (2002) 95-102.
  Sequence
[hsa:50506]
Reference
2  [PMID:11822874]
  Authors
De Deken X, Wang D, Dumont JE, Miot F.
  Title
Characterization of ThOX proteins as components of the thyroid H(2)O(2)-generating system.
  Journal
Exp. Cell. Res. 273 (2002) 187-96.
  Sequence
[hsa:53905 50506]
Reference
3  [PMID:10806195]
  Authors
De Deken X, Wang D, Many MC, Costagliola S, Libert F, Vassart G, Dumont JE, Miot F.
  Title
Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family.
  Journal
J. Biol. Chem. 275 (2000) 23227-33.
  Sequence
[hsa:53905 50506]
Reference
4  [PMID:10601291]
  Authors
Dupuy C, Ohayon R, Valent A, Noel-Hudson MS, Deme D, Virion A.
  Title
Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cdnas.
  Journal
J. Biol. Chem. 274 (1999) 37265-9.
  Sequence
[hsa:50506]
Reference
5  [PMID:10401672]
  Authors
Leseney AM, Deme D, Legue O, Ohayon R, Chanson P, Sales JP, Pires de Carvalho D, Dupuy C, Virion A.
  Title
Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue.
  Journal
Biochimie. 81 (1999) 373-80.
Reference
6  [PMID:1995628]
  Authors
Dupuy C, Virion A, Ohayon R, Kaniewski J, Deme D, Pommier J.
  Title
Mechanism of hydrogen peroxide formation catalyzed by NADPH oxidase in thyroid plasma membrane.
  Journal
J. Biol. Chem. 266 (1991) 3739-43.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
77106-92-4

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