Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With a cytochrome as acceptor

nitric-oxide:ferricytochrome-c oxidoreductase

nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+ [RN:R00783]

R00783 > R00784;
(other) R00081 R00082 R00280 R00785

nitric oxide [CPD:C00533];
H2O [CPD:C00001];
ferricytochrome c [CPD:C00125]

The reaction is catalysed by two types of enzymes, found in the perimplasm of denitrifying bacteria. One type comprises proteins containing multiple copper centres, the other a heme protein, cytochrome cd1. Acceptors include c-type cytochromes such as cytochrome c-550 or cytochrome c-551 from Paracoccus denitrificans or Pseudomonas aeruginosa, and small blue copper proteins such as azurin and pseudoazurin. Cytochrome cd1 also has oxidase and hydroxylamine reductase activities. May also catalyse the reaction of hydroxylamine reductase (EC 1.7.99.1) since this is a well-known activity of cytochrome cd1.

EC 1.7.2.1 created 1961, modified 1976, modified 2001, modified 2002 (EC 1.7.99.3 created 1961 as EC 1.6.6.5, transferred 1964 to EC 1.7.99.3, modified 1976, incorporated 2002, EC 1.9.3.2 created 1965, incorporated 2002)

1  [PMID:5354021]

Miyata M, Mori T.

Studies on denitrification. X. The "denitrifying enzyme" as a nitrite reductase and the electron donating system for denitrification.

J. Biochem. (Tokyo). 66 (1969) 463-71.

2  [PMID:13295215]

CHUNG CW, NAJJAR VA.

Cofactor requirements for enzymatic denitrification.  I.  Nitrite reductase.

J. Biol. Chem. 218 (1956) 617-25.

3  [PMID:13782716]

WALKER GC, NICHOLAS DJ.

Nitrite reductase from Pseudomonas aeruginosa.

Biochim. Biophys. Acta. 49 (1961) 350-60.

4

Singh, J.

Cytochrome oxidase from Pseudomonas aeruginosa. III. Reduction of hydroxylamine.

Biochim. Biophys. Acta 333 (1974) 28-36.

5

Michalski, W.P. and Nicholas, D.J.D.

Molecular characterization of a copper-containing nitrite reductase from Rhodopseudomonas sphaeriodes forma sp. Denitrificans.

Biochim. Biophys. Acta 828 (1985) 130-137.

6  [PMID:1862344]

Godden JW, Turley S, Teller DC, Adman ET, Liu MY, Payne WJ, LeGall J.

The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes.

Science. 253 (1991) 438-42.

[up:P25006]

7  [PMID:7583671]

Williams PA, Fulop V, Leung YC, Chan C, Moir JW, Howlett G, Ferguson SJ, Radford SE, Hajdu J.

Pseudospecific docking surfaces on electron transfer proteins as illustrated by pseudoazurin, cytochrome c550 and cytochrome cd1 nitrite reductase.

Nat. Struct. Biol. 2 (1995) 975-82.

8  [PMID:8639023]

Hole UH, Vollack KU, Zumft WG, Eisenmann E, Siddiqui RA, Friedrich B, Kroneck PM.

Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans.

Arch. Microbiol. 165 (1996) 55-61.

[tbd:Tbd_0077]

9  [PMID:9409151]

Zumft WG.

Cell biology and molecular basis of denitrification.

Microbiol. Mol. Biol. Rev. 61 (1997) 533-616.

10 [PMID:9667932]

Ferguson SJ.

Nitrogen cycle enzymology.

Curr. Opin. Chem. Biol. 2 (1998) 182-93.

11 [PMID:9308169]

Vijgenboom E, Busch JE, Canters GW.

In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression is under control of rpoS and ANR.

Microbiology. 143 ( Pt 9) (1997) 2853-63.