| Entry |
|
|---|
| Name |
nitroalkane oxidase;
nitroethane oxidase;
NAO;
nitroethane:oxygen oxidoreductase |
|---|
| Class |
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
 |
|---|
| Sysname |
nitroalkane:oxygen oxidoreductase |
|---|
| Reaction(IUBMB) |
a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2
[RN:R00799] |
|---|
| Reaction(KEGG) |
R00799
 |
|---|
| Substrate |
nitroalkane [CPD:C06058];
H2O [CPD:C00001];
O2 [CPD:C00007] |
|---|
| Product |
aldehyde [CPD:C00071];
ketone [CPD:C01450];
nitrite [CPD:C00088];
H2O2 [CPD:C00027] |
|---|
| Comment |
Has an absolute requirement for FAD [4]. While nitroethane may be
the physiological substrate [2], the enzyme also acts on several
other nitroalkanes, including 1-nitropropane, 2-nitropropane,
1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and
some nitroalkanols [4]. Differs from EC 1.13.11.16, nitronate
monooxygenase, in that the preferred substrates are neutral
nitroalkanes rather than anionic nitronates [4]. |
|---|
| Pathway |
PATH: ec00910 Nitrogen metabolism |
|---|
| Structures |
PDB: 2REH 2ZAF 3BW2 3BW3 3BW4 3D9D 3D9E 3D9F 3D9G |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:14907722]
LITTLE HN.
Oxidation of nitroethane by extracts from Neurospora.
J. Biol. Chem. 193 (1951) 347-58.
Neurospora crassa [GN:ncr] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:22538]
Kido T, Hashizume K, Soda K.
Purification and properties of nitroalkane oxidase from Fusarium
oxysporum.
J. Bacteriol. 133 (1978) 53-8.
Fusarium oxysporum |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:11867731]
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF.
Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a
new member of the acyl-CoA dehydrogenase superfamily.
Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 2702-7.
Fusarium oxysporum |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:15581574]
Fitzpatrick PF, Orville AM, Nagpal A, Valley MP.
Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to
acyl-CoA dehydrogenase.
Arch. Biochem. Biophys. 433 (2005) 157-65.
Fusarium oxysporum |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:15713081]
Valley MP, Tichy SE, Fitzpatrick PF.
Establishing the kinetic competency of the cationic imine
intermediate in nitroalkane oxidase.
J. Am. Chem. Soc. 127 (2005) 2062-6.
Fusarium oxysporum |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.7.3.1
IUBMB Enzyme Nomenclature: 1.7.3.1
ExPASy - ENZYME nomenclature database: 1.7.3.1
BRENDA, the Enzyme Database: 1.7.3.1
CAS: 9029-36-1 65802-82-6 |
|---|
