KEGG   ENZYME: 1.8.98.2Help
Entry
EC 1.8.98.2                 Enzyme                                 
Name
sulfiredoxin;
Srx1;
sulphiredoxin;
peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With other, known, acceptors
BRITE hierarchy
Sysname
peroxiredoxin-(S-hydroxy-S-oxocysteine):thiol oxidoreductase [ATP-hydrolysing; peroxiredoxin-(S-hydroxycysteine)-forming]
Reaction(IUBMB)
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH = peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
Substrate
peroxiredoxin-(S-hydroxy-S-oxocysteine);
ATP [CPD:C00002];
RSH [CPD:C01336]
Product
peroxiredoxin-(S-hydroxycysteine);
ADP [CPD:C00008];
phosphate [CPD:C00009];
R-S-S-R
Comment
In the course of the reaction of EC 1.11.1.15, peroxiredoxin, its cysteine residue is alternately oxidized to the sulfenic acid, S-hydroxycysteine, and reduced back to cysteine. Occasionally the S-hydroxycysteine residue is further oxidized to the sulfinic acid S-hydroxy-S-oxocysteine, thereby inactivating the enzyme. The reductase provides a mechanism for regenerating the active form of peroxiredoxin, i.e. the peroxiredoxin-(S-hydroxycysteine) form. Apparently the reductase first catalyses the phosphorylation of the -S(O)-OH group by ATP to give -S(O)-O-P, which is attached to the peroxiredoxin by a cysteine residue, forming an -S(O)-S- link between the two enzymes. Attack by a thiol splits this bond, leaving the peroxiredoxin as the sulfenic acid and the reductase as the thiol.
Orthology
K12260  
sulfiredoxin
Genes
HSA: 
140809(SRXN1)
PTR: 
469853(SRXN1)
PPS: 
100983325(SRXN1)
GGO: 
101149749(SRXN1)
PON: 
100437984(SRXN1)
MCC: 
714997(SRXN1)
MMU: 
76650(Srxn1)
RNO: 
296271(Srxn1)
CFA: 
AML: 
FCA: 
101090507(SRXN1)
BTA: 
531606(SRXN1)
SSC: 
100517064 100517736
ECB: 
100067865
MDO: 
100012125
SHR: 
100916952(SRXN1)
OAA: 
100089356
GGA: 
100858692
TGU: 
100222859(SRXN1)
DRE: 
100331561(srxn1)
TRU: 
101063345
OLA: 
101155850
BFO: 
BRAFLDRAFT_215638
CIN: 
100185108
SPU: 
764239
DME: 
Dmel_CG6762
DPO: 
Dpse_GA19844
DAN: 
Dana_GF21110
DER: 
Dere_GG19136
DPE: 
Dper_GL14755
DSE: 
Dsec_GM22861
DSI: 
Dsim_GD17370
DWI: 
Dwil_GK25764
DYA: 
Dyak_GE17693
DGR: 
Dgri_GH12141
DMO: 
Dmoj_GI16197
DVI: 
Dvir_GJ15889
AAG: 
AaeL_AAEL003599
CQU: 
CpipJ_CPIJ009003
NVI: 
100114376(NV11184)
TCA: 
663169
API: 
100161553
PHU: 
Phum_PHUM488840
ISC: 
IscW_ISCW024306
NVE: 
NEMVE_v1g87155
CRE: 
CHLREDRAFT_96296(SRX1)
VCN: 
VOLCADRAFT_105833
SCE: 
YKL086W(SRX1)
AGO: 
AGOS_ADR152CA
ERC: 
Ecym_3406
KLA: 
KLLA0F25938g
LTH: 
KLTH0C01144g
PPA: 
PAS_chr2-1_0236
VPO: 
Kpol_1073p6
ZRO: 
ZYRO0E00858g
CGR: 
CAGL0L05258g
NCS: 
NCAS_0H03330(NCAS0H03330)
NDI: 
NDAI_0C00330(NDAI0C00330)
TPF: 
TPHA_0L02000(TPHA0L02000)
TBL: 
TBLA_0A00770(TBLA0A00770)
TDL: 
TDEL_0B07360(TDEL0B07360)
KAF: 
KAFR_0C03950(KAFR0C03950)
DHA: 
DEHA2C06270g
PIC: 
PICST_34284
PGU: 
PGUG_05341
LEL: 
LELG_00954
CAL: 
CaO19.11021 CaO19.3537
CTP: 
CTRG_01656
CDU: 
CD36_19570
COT: 
CORT_0A09415
YLI: 
YALI0D16709g
CLU: 
CLUG_02779
SPO: 
SPBC106.02c(srx1)
CNB: 
CNBA6150
NGR: 
NAEGRDRAFT_67173
DPP: 
DICPUDRAFT_12802
 » show all
Taxonomy
Reference
1  [PMID:14586471]
  Authors
Biteau B, Labarre J, Toledano MB.
  Title
ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin.
  Journal
Nature. 425 (2003) 980-4.
  Organism
Saccharomyces cerevisiae [GN:sce]
Reference
2  [PMID:15448164]
  Authors
Chang TS, Jeong W, Woo HA, Lee SM, Park S, Rhee SG.
  Title
Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine.
  Journal
J. Biol. Chem. 279 (2004) 50994-1001.
  Organism
Homo sapiens [GN:hsa], Mus musculus [GN:mmu], Rattus norvegicus [GN:rno]
Reference
3  [PMID:15590625]
  Authors
Woo HA, Jeong W, Chang TS, Park KJ, Park SJ, Yang JS, Rhee SG.
  Title
Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-cys peroxiredoxins.
  Journal
J. Biol. Chem. 280 (2005) 3125-8.
  Organism
Homo sapiens [GN:hsa], Rattus norvegicus [GN:rno]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
710319-61-2
DBGET integrated database retrieval system