| Entry |
|
|---|
| Name |
precorrin-3B C17-methyltransferase;
precorrin-3 methyltransferase;
CobJ |
|---|
| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
 |
|---|
| Sysname |
S-adenosyl-L-methionine:precorrin-3B C17-methyltransferase |
|---|
| Reaction(IUBMB) |
S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine +
precorrin-4 [RN:R05180] |
|---|
| Reaction(KEGG) |
R05180;
(other) R05809
 |
|---|
| Substrate |
S-adenosyl-L-methionine [CPD:C00019];
precorrin 3B [CPD:C06406] |
|---|
| Product |
S-adenosyl-L-homocysteine [CPD:C00021];
precorrin 4 [CPD:C06407] |
|---|
| Comment |
In the aerobic cobalamin biosythesis pathway, four enzymes are
involved in the conversion of precorrin-3A to precorrin-6A. The
first of the four steps is carried out by EC 1.14.13.83,
precorrin-3B synthase (CobG), yielding precorrin-3B as the product.
This is followed by three methylation reactions, which introduce a
methyl group at C-17 (CobJ; EC 2.1.1.131), C-11 (CobM; EC 2.1.1.133)
and C-1 (CobF; EC 2.1.1.152) of the macrocycle, giving rise to
precorrin-4, precorrin-5 and precorrin-6A, respectively. |
|---|
| Pathway |
PATH: ec00860 Porphyrin and chlorophyll metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K05934 precorrin-3B C17-methyltransferase |
|---|
| Genes |
STY: STY2232(cbiH)
STT: t0847(cbiH)
SPT: SPA0844(cbiH)
SEK: SSPA0789
SPQ: SPAB_01078
SEI: SPC_1688(cbiH)
SEC: SC2035(cbiH)
SEH: SeHA_C2249(cobJ)
SEE: SNSL254_A2203(cobJ)
SEW: SeSA_A2196(cobJ)
SEA: SeAg_B2149(cobJ)
SED: SeD_A2362(cobJ)
SEG: SG2052(cbiH)
SET: SEN2025(cbiH)
SES: SARI_00861
STM: STM2027(cbiH)
YEN: YE2718(cbiH)
PLU: plu2991(cbiH)
PAY: PAU_01609(cbiH)
KPN: KPN_03192(cbiH)
KPE: KPK_0924(cobJ)
KPU: KP1_4457
CKO: CKO_00811
EIC: NT01EI_2185
PAE: PA2903(cobJ)
PAU: PA14_26530(cobJ)
PAP: PSPA7_2251(cobJ)
PAG: PLES_21611(cobJ)
PPU: PP_4826(cobJ)
PPF: Pput_4704
PPG: PputGB1_4882
PPW: PputW619_4616
PST: PSPTO_4874
PSB: Psyr_4414
PSP: PSPPH_4457
PFL: PFL_0660
PFO: Pfl01_0607
PFS: PFLU0607
PEN: PSEEN4868(cobJ)
PMY: Pmen_4574
SSE: Ssed_2086
MCA: MCA2293
HHA: Hhal_1344
HCH: HCH_06478(cobJ)
MMW: Mmwyl1_1374
TAU: Tola_1673
CVI: CV_1562(cbiJ)
RSO: RSp0618(cbiJH)
BMA: BMA1164(cbiG)
BMV: BMASAVP1_A1605(cbiG)
BML: BMA10229_A0267
BMN: BMA10247_0893(cbiG)
BPS: BPSL1762(cobJ)
BPM: BURPS1710b_2110(cobJ)
BPL: BURPS1106A_1964(cbiG)
BPD: BURPS668_1947(cbiG)
BPR: GBP346_A1988
BTE: BTH_I2401
BVI: Bcep1808_1607
BUR: Bcep18194_A4821
BCN: Bcen_1191
BCH: Bcen2424_1671
BCM: Bcenmc03_1644
BCJ: BCAL1723(cobJ)
BAM: Bamb_1571
BAC: BamMC406_1589
BMU: Bmul_1571
BMJ: BMULJ_01673(cobJ)
BXE: Bxe_B1242
BPH: Bphy_3146
BPY: Bphyt_5372
BGL: bglu_1g18460
POL: Bpro_2767
PNA: Pnap_2153
AAV: Aave_0077
MPT: Mpe_B0447(cobJ) Mpe_B0480(cbiG)
AZO: azo3537(cbiH)
DAR: Daro_1685
APP: CAP2UW1_2611
PCA: Pcar_0471
DVU: DVU3170(cobJ)
DVL: Dvul_0216
DDE: Dde_3181
DPS: DP0224
MLO: mlr1381
SME: SMc03190(cobJ)
SMD: Smed_2815
ATU: Atu2800(cobJ)
ATC: AGR_C_5079
AVI: Avi_2636(cobJ)
RET: RHE_CH02486(cobE) RHE_PE00452(cobJ)
RLE: RL2826(cobE) pRL110628(cobJ)
BME: BMEI0700 BMEI0712
BMF: BAB1_1307 BAB1_1321
BMB: BruAb1_1290 BruAb1_1302
BMC: BAbS19_I12200
BMS: BR1289 BR1301
BMT: BSUIS_A1339(cobJ)
BOV: BOV_1252
BCS: BCAN_A1312(cobJ)
BMR: BMI_I1302(cobJ)
OAN: Oant_1896
BJA: blr3267(cobJ)
BRA: BRADO4903(cobJ)
BBT: BBta_3148(cobJ)
RPA: RPA2088(cobJ)
RPB: RPB_3180
RPC: RPC_1886
RPD: RPD_2318
RPE: RPE_2223
XAU: Xaut_3282
AZC: AZC_3939
MEX: Mext_1435
MDI: METDI2096(cobJ)
MRD: Mrad2831_3119
MET: M446_2442
MCH: Mchl_5689
BID: Bind_3511
SIL: SPO2867(cobJ)
SIT: TM1040_2214
RSP: RSP_2824(cobJ)
RSH: Rsph17029_1471
RSQ: Rsph17025_1521
JAN: Jann_2927
RDE: RD1_3823(cobJ)
PDE: Pden_2536
DSH: Dshi_0175(cbiG)
NAR: Saro_0338
GBE: GbCGDNIH1_0662
RRU: Rru_A2989
MAG: amb0298
MGM: Mmc1_3128
GKA: GK1805
GTN: GTNG_1694(cbiH)
LMO: lmo1199(cbiH)
LMF: LMOf2365_1208(cobJ)
LIN: lin1162
LWE: lwe1156(cbiH)
LSP: Bsph_3446
SSA: SSA_0472
LRE: Lreu_1714
CAC: CAC1382(cbiH)
CPE: CPE1220(cbiH)
CPF: CPF_1428(cobJ)
CPR: CPR_1234(cobJ)
CTC: CTC00739
CNO: NT01CX_0575(cobJ)
CDF: CD3424(cbiH)
CBO: CBO0949(cbiH)
CBK: CLL_A2930(cobJ)
CBE: Cbei_1257
CKL: CKL_0730(cbiH)
CPY: Cphy_1378
CCE: Ccel_1275
AMT: Amet_0076
AOE: Clos_1021
DSY: DSY4066(cobJ)
DRM: Dred_2709
PTH: PTH_0956
HMO: HM1_2391(cbiH)
EEL: EUBELI_20183
TEX: Teth514_0315
CHY: CHY_0764(cobJ)
MTA: Moth_1094
MTU: Rv2066(cobI)
MTC: MT2126(cobIJ)
MRA: MRA_2080(cobI)
MTF: TBFG_12102
MBO: Mb2092(cobI)
MBB: BCG_2085(cobI)
MPA: MAP1812(cobI)
MAV: MAV_2430
MSM: MSMEG_3873
MUL: MUL_2290(cobI)
MVA: Mvan_3431
MGI: Mflv_3104
MAB: MAB_2198c
MMC: Mmcs_2487
MKM: Mkms_2532
MJL: Mjls_2524
MMI: MMAR_3046(cobI)
CDI: DIP1233(cobIJ)
NFA: nfa31520(cobIJ)
RHA: RHA1_ro00174
SCO: SCO1857(SCI39.04)
SMA: SAV_6407(cobJ)
SGR: SGR_3822
NCA: Noca_2891 Noca_2892 Noca_3409
TFU: Tfu_0316
TCU: Tcur_1317
FRA: Francci3_1521
FAL: FRAAL2333(cobI)
SEN: SACE_5951 SACE_5960(cobJ)
AMI: Amir_5856
STP: Strop_2520
SAQ: Sare_2702
CAI: Caci_6033
RXY: Rxyl_0643
AFO: Afer_0802
TDE: TDE0623(cobJ)
LIL: LB157(cbiH)
LIC: LIC20127(cobJ)
LBJ: LBJ_4185(cobJ)
LBL: LBL_4200(cobJ)
BFR: BF2518
BFS: BF2547(cobJ)
BVU: BVU_3083
PGI: PG0213
PDI: BDI_1067
FNU: FN0951
TAI: Taci_0049
SYN: slr0969(cbiH)
SYW: SYNW2126(cbiG)
SYC: syc2240_c(cobJ)
SYF: Synpcc7942_1854
SYD: Syncc9605_0329
SYE: Syncc9902_1997
SYG: sync_0391(cbiG)
SYR: SynRCC307_2160(cbiG)
SYX: SynWH7803_0388(cbiG)
CYA: CYA_1591(cbiG) CYA_1847(cbiG)
CYB: CYB_0397(cbiG)
TEL: tll1307(cobJ)
MAR: MAE_42070(cobJ)
CYT: cce_0926(cbiGH)
CYP: PCC8801_3628
CYC: PCC7424_0379
CYN: Cyan7425_1675
CYH: Cyan8802_2485
GVI: gvip349(cobJ)
ANA: all0453
NPU: Npun_R4781
AVA: Ava_2863
PMA: Pro1671(cobJ)
PMM: PMM1525(cbiG)
PMT: PMT1772(cbiG)
PMN: PMN2A_1081
PMI: PMT9312_1617
PMB: A9601_17291(cobJ)
PMC: P9515_17041(cobJ)
PMF: P9303_23501(cobJ)
PMG: P9301_17171(cobJ)
PMH: P9215_17931(cobJ)
PMJ: P9211_16381(cobJ)
PME: NATL1_19561(cobJ)
TER: Tery_4366
AMR: AM1_4688
CTE: CT0387(cbiCH)
RRS: RoseRS_0572
RCA: Rcas_0637
CAU: Caur_2565
CAG: Cagg_1264
CHL: Chy400_2772
DGE: Dgeo_2355
TTH: TT_P0013
TTJ: TTHB056
MJA: MJ0813
MMP: MMP0953(cbiH)
MMQ: MmarC5_0760
MAC: MA4259(cbiH)
MBA: Mbar_A0629
MMA: MM_0995
MBU: Mbur_2356
MHU: Mhun_3213
MLA: Mlab_1080
MEM: Memar_0512
MBN: Mboo_1226
MPL: Mpal_1719
MTH: MTH1403
MST: Msp_1582(cbiH)
MSI: Msm_1273
MKA: MK1376(cobJ)
AFU: AF0724(cbiH)
HAL: VNG1555G(cobH) VNG1557G(cbiH)
HMA: rrnAC3005(cobH) rrnAC3008(cbiH)
HWA: HQ3033A(cbiH2) HQ3034A(cbiH1)
NPH: NP1114A(cbiH_2) NP1116A(cbiH_1)
HLA: Hlac_3473 Hlac_3474
TAC: Ta0655
TVO: TVN0921
PTO: PTO0993
SSO: SSO2306(cbiH)
STO: ST1823
SAI: Saci_0398
SIS: LS215_0112
SIA: M1425_0112
SIM: M1627_0112
SID: M164_0114
SIY: YG5714_0114
SIN: YN1551_0112
MSE: Msed_2092
PAI: PAE0344
PCL: Pcal_1525
TNE: Tneu_0297
NMR: Nmar_1752
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:8405386]
Scott AI, Roessner CA, Stolowich NJ, Spencer JB, Min C, Ozaki SI.
Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative
ring contraction and insertion of the fourth methyl group.
FEBS. Lett. 331 (1993) 105-8.
Pseudomonas denitrificans |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:8226690]
Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F.
Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas
denitrificans.
J. Bacteriol. 175 (1993) 7430-40.
Pseudomonas denitrificans |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 2.1.1.131
IUBMB Enzyme Nomenclature: 2.1.1.131
ExPASy - ENZYME nomenclature database: 2.1.1.131
BRENDA, the Enzyme Database: 2.1.1.131
CAS: 152787-64-9 |
|---|
