| Entry |
|
|---|
| Name |
precorrin-6A synthase (deacetylating);
precorrin-6X synthase (deacetylating);
CobF |
|---|
| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
 |
|---|
| Sysname |
S-adenosyl-L-methionine:precorrin-5 C1-methyltransferase
(deacetylating) |
|---|
| Reaction(IUBMB) |
S-adenosyl-L-methionine + precorrin-5 + H2O =
S-adenosyl-L-homocysteine + precorrin-6A + acetate [RN:R05219] |
|---|
| Reaction(KEGG) |
R05219
 |
|---|
| Substrate |
S-adenosyl-L-methionine [CPD:C00019];
precorrin 5 [CPD:C06416];
H2O [CPD:C00001] |
|---|
| Product |
S-adenosyl-L-homocysteine [CPD:C00021];
precorrin 6A [CPD:C06320];
acetate [CPD:C00033] |
|---|
| Comment |
In the aerobic cobalamin biosythesis pathway, four enzymes are
involved in the conversion of precorrin-3A to precorrin-6A. The
first of the four steps is carried out by EC 1.14.13.83,
precorrin-3B synthase (CobG), yielding precorrin-3B as the product.
This is followed by three methylation reactions, which introduce a
methyl group at C-17 (CobJ; EC 2.1.1.131), C-11 (CobM; EC 2.1.1.133)
and C-1 (CobF; EC 2.1.1.152) of the macrocycle, giving rise to
precorrin-4, precorrin-5 and precorrin-6A, respectively. |
|---|
| Pathway |
PATH: ec00860 Porphyrin and chlorophyll metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K02228 precorrin-6A synthase |
|---|
| Genes |
PPU: PP_3763
PPF: Pput_2000
PPG: PputGB1_2140
PPW: PputW619_2104
PST: PSPTO_2709
PSB: Psyr_2442
PSP: PSPPH_2598
PFL: PFL_2249
PFO: Pfl01_2061
PFS: PFLU2445(cobF)
PEN: PSEEN3208
PMY: Pmen_4583
BGL: bglu_2g11970
DAC: Daci_5843
CTT: CtCNB1_1870
MLO: mlr1461
SME: SMc03194(cobF)
SMD: Smed_2819
ARA: Arad_9597(cobF)
RET: RHE_PE00456(cobF)
REC: RHECIAT_PA0000379(cobF)
RLE: pRL110632(cobF)
RLT: Rleg2_6018
RLG: Rleg_7173
RHI: NGR_c29820(cobF)
BJA: blr3275(cobF)
RPA: RPA2097(cobF)
RPB: RPB_3174
RPC: RPC_1896
RPD: RPD_2326
RPE: RPE_2230
RPT: Rpal_2387
MEX: Mext_3385
MEA: Mex_1p3613(cobF)
MDI: METDI4188(cobF)
MRD: Mrad2831_1538
MPO: Mpop_3576
MCH: Mchl_3694
MSL: Msil_3231
SIL: SPO2874(cobF)
SIT: TM1040_2224
RSP: RSP_2819(cobF)
RSH: Rsph17029_1477
RSQ: Rsph17025_1527
RSK: RSKD131_1144
JAN: Jann_2920
RDE: RD1_3816(cobF)
PDE: Pden_2542
NAR: Saro_0332
RRU: Rru_A2970
MPA: MAP0888
MAV: MAV_1065(cobF)
MSM: MSMEG_5548(cobF)
MUL: MUL_4422
MVA: Mvan_0376
MGI: Mflv_0361
MAB: MAB_1037
MMC: Mmcs_4338
MKM: Mkms_4424
MJL: Mjls_4718
MMI: MMAR_4563
CDI: DIP0822
CKP: ckrop_0458(cobF)
NFA: nfa50210
RHA: RHA1_ro02247
RER: RER_44600(cobF)
ROP: ROP_19610(cobF)
GBR: Gbro_1321
SCO: SCO6971(SC6F7.24c)
SMA: SAV_1600(cobF)
SGR: SGR_1214
PAC: PPA1920
NCA: Noca_2879
TFU: Tfu_0320
FRA: Francci3_2657
FAL: FRAAL2802
NML: Namu_0142
SVI: Svir_17580
AMI: Amir_4201
CAI: Caci_6988
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:8226690]
Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F.
Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas
denitrificans.
J. Bacteriol. 175 (1993) 7430-40.
Pseudomonas denitrificans |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:12195810]
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
The biosynthesis of adenosylcobalamin (vitamin B12).
Nat. Prod. Rep. 19 (2002) 390-412.
Pseudomonas denitrificans |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 2.1.1.152
IUBMB Enzyme Nomenclature: 2.1.1.152
ExPASy - ENZYME nomenclature database: 2.1.1.152
BRENDA, the Enzyme Database: 2.1.1.152 |
|---|
