KEGG   ENZYME: 2.1.1.41Help
Entry
EC 2.1.1.41                 Enzyme                                 

Name
sterol 24-C-methyltransferase;
Delta24-methyltransferase;
Delta24-sterol methyltransferase;
zymosterol-24-methyltransferase;
S-adenosyl-4-methionine:sterol Delta24-methyltransferase;
SMT1;
24-sterol C-methyltransferase;
S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase;
phytosterol methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol [RN:R04427]
Reaction(KEGG)
R04427;
(other) R07481
Show
Substrate
S-adenosyl-L-methionine [CPD:C00019];
5alpha-cholesta-8,24-dien-3beta-ol [CPD:C05437]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
24-methylene-5alpha-cholest-8-en-3beta-ol [CPD:C04525]
Comment
Requires glutathione. Acts on a range of sterols with a 24(25)-double bond in the sidechain. While zymosterol is the preferred substrate it also acts on desmosterol, 5alpha-cholesta-7,24-dien-3beta-ol, 5alpha-cholesta-5,7,24-trien-3beta-ol, 4alpha-methylzymosterol and others. S-Adenosyl-L-methionine attacks the Si-face of the 24(25) double bond and the C-24 hydrogen is transferred to C-25 on the Re face of the double bond.
Pathway
Steroid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00559  
sterol 24-C-methyltransferase
Genes
AQU: 
ATH: 
AT5G13710(SMT1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
GMX: 
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
POPTR_0009s06360g(POPTRDRAFT_1087868) POPTR_942143
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os03t0807600-00(Os03g0807600) Os07t0206700-01(Os07g0206700)
BDI: 
SBI: 
SORBI_01g004290(SORBIDRAFT_01g004290) SORBI_09g029600(SORBIDRAFT_09g029600)
ZMA: 
100126899(smt1) 100282501 100286381(AC210013.4_FG003) 541682(SMT)
SITA: 
SMO: 
PPP: 
OLU: 
OTA: 
MIS: 
MPP: 
CME: 
GSL: 
SCE: 
YML008C(ERG6)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0H02570(NCAS0H02570)
NDI: 
NDAI_0C01050(NDAI0C01050)
TPF: 
TPHA_0D02350(TPHA0D02350)
TBL: 
TBLA_0D01630(TBLA0D01630)
TDL: 
TDEL_0A03930(TDEL0A03930)
KAF: 
KAFR_0C05410(KAFR0C05410)
DHA: 
PIC: 
PGU: 
LEL: 
CAL: 
CTP: 
CDU: 
COT: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
MGR: 
FGR: 
NHE: 
VAL: 
SSL: 
BFU: 
ANI: 
NFI: 
AFM: 
AOR: 
AOR_1_1074144(AO090023000624) AOR_1_480054(AO090011000289)
ANG: 
ANI_1_256124(An14g01590)
AFV: 
ACT: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
ZTR: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
PPL: 
LBC: 
MPR: 
CCI: 
SCM: 
UMA: 
MGL: 
PGR: 
MBR: 
DDI: 
DPP: 
DFA: 
DFA_09943(smt1)
ACAN: 
PTI: 
TPS: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
SRI: 
 » show all
Taxonomy
Reference
1  [PMID:5354959]
  Authors
Moore JT, Gaylor JL.
  Title
Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast.
  Journal
J. Biol. Chem. 244 (1969) 6334-40.
  Organism
Saccharomyces cerevisiae [GN:sce]
Reference
2  [PMID:8597586]
  Authors
Venkatramesh M, Guo DA, Jia Z, Nes WD.
  Title
Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.
  Journal
Biochim. Biophys. Acta. 1299 (1996) 313-24.
  Organism
Saccharomyces cerevisiae [GN:sce]
Reference
3
  Authors
Tong, Y., McCourt, B.S., Guo, D., Mangla, A.T., Zhou, W.X., Jenkins, M.D., Zhou, W., Lopez, M. and Nes, W.D.
  Title
, Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana.
  Journal
Tetrahedron Lett. 38 (1997) 6115-6118.
Reference
4  [PMID:9746350]
  Authors
Bouvier-Nave P, Husselstein T, Benveniste P.
  Title
Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis.
  Journal
Eur. J. Biochem. 256 (1998) 88-96.
  Organism
Nicotiana tabacum
  Sequence
[osa:4342690]
Reference
5  [PMID:9606964]
  Authors
Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M.
  Title
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae.
  Journal
Arch. Biochem. Biophys. 353 (1998) 297-311.
  Organism
Saccharomyces cerevisiae [GN:sce]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37257-07-1

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