KEGG   ENZYME: 2.1.1.98Help
Entry
EC 2.1.1.98                 Enzyme                                 
Name
diphthine synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase;
diphthine methyltransferase;
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] methyltransferase
Reaction(IUBMB)
3 S-adenosyl-L-methionine + 2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + 2-[3-carboxy-3-(trimethylammonio)propyl]-L-histidine-[translation elongation factor 2] (overall reaction) [RN:R10306];
(1a) S-adenosyl-L-methionine + 2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[3-carboxy-3-(methylammonio)propyl]-L-histidine-[translation elongation factor 2] [RN:R04481];
(1b) S-adenosyl-L-methionine + 2-[3-carboxy-3-(methylammonio)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[3-carboxy-3-(dimethylammonio)propyl]-L-histidine-[translation elongation factor 2] [RN:R08468];
(1c) S-adenosyl-L-methionine + 2-[3-carboxy-3-(dimethylammonio)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[3-carboxy-3-(trimethylammonio)propyl]-L-histidine-[translation elongation factor 2] [RN:R08469]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2];
2-[3-carboxy-3-(methylammonio)propyl]-L-histidine-[translation elongation factor 2];
2-[3-carboxy-3-(dimethylammonio)propyl]-L-histidine-[translation elongation factor 2]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
2-[3-carboxy-3-(trimethylammonio)propyl]-L-histidine-[translation elongation factor 2];
2-[3-carboxy-3-(methylammonio)propyl]-L-histidine-[translation elongation factor 2];
2-[3-carboxy-3-(dimethylammonio)propyl]-L-histidine-[translation elongation factor 2]
Comment
The trimethylated product, diphthine, is converted into diphthamide by EC 6.3.1.14, diphthine---ammonia ligase. The relevant histidine of EF2 is His715 in mammals, His699 in yeast and His600 in Pyrococcus horikoshii.
Orthology
K00586  
diphthine synthase
Genes
HSA: 
51611(DPH5)
PTR: 
457062(DPH5)
PPS: 
100976633(DPH5)
GGO: 
101127053(DPH5)
PON: 
100453387(DPH5)
MCC: 
712253(DPH5)
MMU: 
69740(Dph5)
RNO: 
295394(Dph5)
CFA: 
612092(DPH5)
AML: 
FCA: 
101090957(DPH5)
BTA: 
508904(DPH5)
SSC: 
100157715(DPH5)
ECB: 
100050115
MDO: 
100032968
SHR: 
100914997(DPH5)
OAA: 
100074886
GGA: 
424463(DPH5)
MGP: 
100549424
TGU: 
100219750(DPH5)
ACS: 
100562876
XLA: 
380263(dph5)
XTR: 
448611(dph5)
DRE: 
100003898(dph5) 492514(dph5)
TRU: 
101065858
OLA: 
101171985
BFO: 
BRAFLDRAFT_126365
CIN: 
100181968
SPU: 
577892 753556
DME: 
Dmel_CG31289(Dph5)
DPO: 
Dpse_GA16151
DAN: 
Dana_GF16145
DER: 
Dere_GG12504
DPE: 
Dper_GL13679
DSE: 
Dsec_GM23636
DSI: 
Dsim_GD18446
DWI: 
Dwil_GK11785
DYA: 
Dyak_GE24024(Dyak_Dph5)
DGR: 
Dgri_GH18052
DMO: 
Dmoj_GI10523
DVI: 
Dvir_GJ24174
AGA: 
AgaP_AGAP004412
AAG: 
AaeL_AAEL002808
CQU: 
CpipJ_CPIJ008675
AME: 
412557(Dph5)
NVI: 
100120480(DPH5)
TCA: 
655410
API: 
100160206
PHU: 
Phum_PHUM004200
ISC: 
IscW_ISCW015932
CEL: 
CELE_B0491.7
CBR: 
CBG02918
BMY: 
Bm1_10785
TSP: 
Tsp_10674
SMM: 
Smp_025570.2
NVE: 
NEMVE_v1g93189
AQU: 
100639400
ATH: 
AT4G31790
ALY: 
ARALYDRAFT_656871
GMX: 
100784092 100787586
MTR: 
MTR_3g113750
CSV: 
101207207
RCU: 
RCOM_1597420
POP: 
POPTR_1071060
VVI: 
100242000
OSA: 
4332866
DOSA: 
Os03t0355800-01(Os03g0355800)
BDI: 
100846135
SBI: 
SORBI_01g035110(SORBIDRAFT_01g035110)
ZMA: 
100191377(pco083348)
SMO: 
SELMODRAFT_232025 SELMODRAFT_232835
PPP: 
PHYPADRAFT_198807
CRE: 
CHLREDRAFT_122908
VCN: 
VOLCADRAFT_109194
OLU: 
OSTLU_40543
CME: 
CMJ181C
SCE: 
YLR172C(DPH5)
AGO: 
AGOS_AFR123W
ERC: 
Ecym_2725
KLA: 
KLLA0F22836g
LTH: 
KLTH0H11748g
PPA: 
PAS_chr2-1_0577
VPO: 
Kpol_1061p22
ZRO: 
ZYRO0E05258g
CGR: 
CAGL0B04961g
NCS: 
NCAS_0A08800(NCAS0A08800)
NDI: 
NDAI_0G05220(NDAI0G05220)
TPF: 
TPHA_0B03580(TPHA0B03580)
TBL: 
TBLA_0C01150(TBLA0C01150)
TDL: 
TDEL_0B06200(TDEL0B06200)
KAF: 
KAFR_0I02070(KAFR0I02070)
DHA: 
DEHA2E23914g
PIC: 
PICST_78373(DPH5)
PGU: 
PGUG_04761
LEL: 
LELG_03884
CAL: 
CaO19.6676(DPH51)
CTP: 
CTRG_05542
CDU: 
CD36_53390 CD36_53440
COT: 
CORT_0E05000
YLI: 
YALI0F17138g
CLU: 
CLUG_05937
NCR: 
NCU07266
SMP: 
SMAC_00801
PAN: 
PODANS72p127
TTT: 
THITE_2114762
MTM: 
MYCTH_2298356
MGR: 
MGG_10496
FGR: 
FG09628.1
NHE: 
NECHADRAFT_58379
VAL: 
VDBG_01098
SSL: 
SS1G_09828
BFU: 
BC1G_15005
ANI: 
AN0720.2
NFI: 
NFIA_011450
AFM: 
AFUA_1G14020
AOR: 
AOR_1_826194(AO090012000475)
ANG: 
ANI_1_1012074(An08g07220)
AFV: 
AFLA_017390
ACT: 
ACLA_021450
PCS: 
Pc16g12550
CIM: 
CIMG_03364
CPW: 
CPC735_008450
PBL: 
PAAG_02178
URE: 
UREG_07192
ABE: 
ARB_00078
TVE: 
TRV_00926
AJE: 
HCAG_06727
PNO: 
SNOG_03141
PTE: 
PTT_18731
ZTR: 
MYCGRDRAFT_93945
TML: 
GSTUM_00008315001
SPO: 
SPCC576.14(dph5)
CNE: 
CNF03650
CNB: 
CNBF1170
CGI: 
CGB_F1570W
PPL: 
POSPLDRAFT_88734
LBC: 
LACBIDRAFT_334053
CCI: 
CC1G_04639
SCM: 
SCHCODRAFT_57471
UMA: 
UM04406.1
MGL: 
MGL_2369
PGR: 
PGTG_12290
ECU: 
ECU11_1430
EIN: 
Eint_111320
EHE: 
EHEL_111320
NCE: 
NCER_101909
MBR: 
MONBRDRAFT_33663
DDI: 
DDB_G0276115(dph5)
DPP: 
DICPUDRAFT_37635
EHI: 
EHI_127240(129.t00009)
EDI: 
EDI_190670
PFA: 
PF10_0087
PFH: 
PFHG_02658
PYO: 
PY01561
PCB: 
PC000390.05.0
PBE: 
PB001435.02.0
PKN: 
PKH_080840
PVX: 
PVX_094665
PCY: 
PCYB_081860
TAN: 
TA03050
TPV: 
TP01_0634
BBO: 
BBOV_IV002920(21.m02908)
CPV: 
cgd6_1200
CHO: 
Chro.60154
TGO: 
TGME49_049920
TET: 
TTHERM_00833850
PTM: 
GSPATT00026998001 GSPATT00028621001
TBR: 
Tb927.4.4650(Tb04.3I12.360)
TCR: 
407477.30 511389.130
LMA: 
LMJF_31_1610
LIF: 
LINJ_31_1640
LDO: 
LDBPK_311640
LMI: 
LMXM_30_1610
LBZ: 
LBRM_31_1820
GLA: 
GL50803_28666
PTI: 
PHATRDRAFT_5236
TPS: 
THAPSDRAFT_20788
PIF: 
PITG_11907
MJA: 
MJ_1274
MFE: 
Mefer_0044
MVU: 
Metvu_1279
MFS: 
MFS40622_0657
MIF: 
Metin_0832
MIG: 
Metig_1174
MMP: 
MMP0588(dph5)
MMQ: 
MmarC5_1019
MMX: 
MmarC6_0305
MMZ: 
MmarC7_1609
MMD: 
GYY_03540
MAE: 
Maeo_0435
MVN: 
Mevan_1459
MVO: 
Mvol_0810
MOK: 
Metok_0033
MAC: 
MA1370(dph5)
MBA: 
Mbar_A2900
MMA: 
MM_2355
MMAZ: 
MmTuc01_2405
MBU: 
Mbur_0971
MMH: 
Mmah_0645
MEV: 
Metev_1417
MZH: 
Mzhil_0765
MPY: 
Mpsy_3166
MHZ: 
Metho_1698
MTP: 
Mthe_1120
MCJ: 
MCON_1449(dphB)
MHI: 
Mhar_0531
MHU: 
Mhun_0788
MLA: 
Mlab_0386
MEM: 
Memar_1783
MBG: 
BN140_0607(dphB)
MPI: 
Mpet_0734
MBN: 
Mboo_2068
MFO: 
Metfor_0785
MPL: 
Mpal_0116
MPD: 
MCP_0924(dphB)
MEZ: 
Mtc_2082
RCI: 
RRC519(dphB)
MTH: 
MTH1874
MMG: 
MTBMA_c04450(dphB)
MST: 
Msp_1017(dphB)
MSI: 
Msm_0801
MRU: 
mru_1764(dphB)
MEL: 
Metbo_0693
MEW: 
MSWAN_1562
MFV: 
Mfer_0700
MKA: 
MK0747(DPH5)
AFU: 
AF0381(dph5)
APO: 
Arcpr_0161
AVE: 
Arcve_1504
AST: 
Asulf_00669
FPL: 
Ferp_1481
HAL: 
VNG1118G(lds)
HSL: 
OE2613R
HMA: 
rrnAC1406(dph5)
HHI: 
HAH_1989(dph5)
HWA: 
HQ1603A(dph5)
HWC: 
Hqrw_1714(dph5)
NPH: 
NP1884A
NMO: 
Nmlp_1959(dph5)
HLA: 
Hlac_0892
HUT: 
Huta_0243
HMU: 
Hmuk_1953
HTU: 
Htur_2752
NMG: 
Nmag_0897
HVO: 
HVO_0916(dph5)
HME: 
HFX_0887(dph5)
HJE: 
HacjB3_08510
HBO: 
Hbor_23080
HXA: 
Halxa_2402
NAT: 
NJ7G_1756
NPE: 
Natpe_0971
NGE: 
Natgr_0089
HRU: 
Halru_1978
NOU: 
Natoc_2717
TAC: 
Ta0883
TVO: 
TVN0982
PTO: 
PTO1233
TAR: 
TALC_01129
PHO: 
PH0725
PAB: 
PAB1501(dph5)
PFU: 
PF0595
PFI: 
PFC_02105
PYN: 
PNA2_1369
PYA: 
PYCH_07540
PYS: 
Py04_0739
TKO: 
TK0106
TON: 
TON_1372
TGA: 
TGAM_0314(dph5)
TSI: 
TSIB_0124
TBA: 
TERMP_01321
THE: 
GQS_08695
THA: 
TAM4_1375
THM: 
CL1_1363
ABI: 
Aboo_0591
ACF: 
AciM339_0455
MAX: 
MMALV_11700
APE: 
APE_0931(dphB)
SMR: 
Smar_0509
SHC: 
Shell_0288
IHO: 
Igni_0657
DKA: 
DKAM_1445
DMU: 
Desmu_0139
DFD: 
Desfe_0069
TAG: 
Tagg_1079
IAG: 
Igag_0048
THG: 
TCELL_0750
HBU: 
Hbut_0128
PFM: 
Pyrfu_1089
SSO: 
SSO0953
SOL: 
Ssol_1929
STO: 
ST1278
SAI: 
Saci_1334
SACN: 
SacN8_06505
SACR: 
SacRon12I_06495
SIS: 
LS215_1360
SIA: 
M1425_1273
SIM: 
M1627_1323
SID: 
M164_1257
SIY: 
YG5714_1258
SIN: 
YN1551_1590
SII: 
LD85_1385
SIH: 
SiH_1218
SIR: 
SiRe_1136
SIC: 
SiL_1133
MSE: 
Msed_1735
MCN: 
Mcup_0493
AHO: 
Ahos_1400
PAI: 
PAE1139
PIS: 
Pisl_1283
PCL: 
Pcal_0705
PAS: 
Pars_0230
PYR: 
P186_2537
POG: 
Pogu_2233
TNE: 
Tneu_0216
CMA: 
Cmaq_1990
TUZ: 
TUZN_1249
TTN: 
TTX_0095(dph5)
VDI: 
Vdis_2028
VMO: 
VMUT_0435
TPE: 
Tpen_0689
ASC: 
ASAC_0414
CLG: 
Calag_1209
FFO: 
FFONT_1021
NMR: 
Nmar_1297
NIR: 
NSED_07195
CSY: 
CENSYa_0126
NGA: 
Ngar_c14540
NEQ: 
NEQ422
HAH: 
Halar_0827
 » show all
Taxonomy
Reference
1  [PMID:3042777]
  Authors
Chen JY, Bodley JW.
  Title
Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase.
  Journal
J. Biol. Chem. 263 (1988) 11692-6.
  Organism
Saccharomyces cerevisiae [GN:sce]
Reference
2  [PMID:3346227]
  Authors
Moehring JM, Moehring TJ.
  Title
The post-translational trimethylation of diphthamide studied in vitro.
  Journal
J. Biol. Chem. 263 (1988) 3840-4.
  Organism
Homo sapiens [GN:hsa], Mus musculus [GN:mmu], Rattus norvegicus [GN:rno], Bos taurus [GN:bta], Gallus gallus [GN:gga], Saccharomyces cerevisiae [GN:sce], Cercopithecus aethiops, Oryctolagus cuniculus, Cricetulus griseus, Mesocricetus auratus, Muntiacus muntjak, Notopthalmus viridescens, Porthetria dispar, Triticum aestivum, Schizophyllum commune
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
114514-25-9
DBGET integrated database retrieval system