| Entry |
|
|---|
| Name |
isopenicillin-N N-acyltransferase;
acyl-coenzyme A:isopenicillin N acyltransferase;
isopenicillin N:acyl-CoA: acyltransferase |
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| Class |
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
 |
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| Sysname |
acyl-CoA:isopenicillin N N-acyltransferase |
|---|
| Reaction(IUBMB) |
phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G +
L-2-aminohexanedioate [RN:R04868] |
|---|
| Reaction(KEGG) |
R04868;
(other) R06361
 |
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| Substrate |
phenylacetyl-CoA [CPD:C00582];
isopenicillin N [CPD:C05557];
H2O [CPD:C00001] |
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| Product |
CoA [CPD:C00010];
penicillin G [CPD:C05551];
L-2-aminohexanedioate [CPD:C00956] |
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| Comment |
Proceeds by a two stage mechanism via 6-aminopenicillanic acid.
Different from EC 3.5.1.11, penicillin amidase. |
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| Pathway |
PATH: ec00311 Penicillin and cephalosporin biosynthesis
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K10852 isopenicillin-N N-acyltransferase |
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| Genes |
YLI: YALI0E30833g
FGR: FG02453.1
ANI: AN2623.2
AFM: AFUA_3G12620
AOR: AO090005000709
ANG: An02g08570
PEN: PSEEN3262
BTE: BTH_II2354
BUR: Bcep18194_B2777
BCN: Bcen_4281
BCH: Bcen2424_4085
BCM: Bcenmc03_3438
BAM: Bamb_3495
BAC: BamMC406_3978
BMU: Bmul_4501
BAV: BAV1029(aat)
POL: Bpro_1442
VEI: Veis_4727
MLO: mll9373
BRA: BRADO2616
MSM: MSMEG_2097
RHA: RHA1_ro03846
SGR: SGR_3008
AAU: AAur_1233
FRA: Francci3_3269
SEN: SACE_5325
SAQ: Sare_1057
BVU: BVU_1032
GFO: GFO_1653
FJO: Fjoh_1076
FPS: FP2254
COC: Coch_1418
CYB: CYB_0863
CAU: Caur_1389
HAU: Haur_4392
 |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:2120195]
Tobin MB, Fleming MD, Skatrud PL, Miller JR.
Molecular characterization of the acyl-coenzyme A:isopenicillin N
acyltransferase gene (penDE) from Penicillium chrysogenum and
Aspergillus nidulans and activity of recombinant enzyme in
Escherichia coli.
J. Bacteriol. 172 (1990) 5908-14.
Aspergillus nidulans [GN:ani], Penicillium chrysogenum [GN:pcs] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:8396910]
Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ.
Investigations into the post-translational modification and
mechanism of isopenicillin N:acyl-CoA acyltransferase using
electrospray mass spectrometry.
Biochem. J. 294 ( Pt 2) (1993) 357-63.
Penicillium chrysogenum [GN:pcs] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 2.3.1.164
IUBMB Enzyme Nomenclature: 2.3.1.164
ExPASy - ENZYME nomenclature database: 2.3.1.164
BRENDA, the Enzyme Database: 2.3.1.164
CAS: 54576-90-8 |
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