KEGG   ENZYME: 2.3.2.16Help
Entry
EC 2.3.2.16                 Enzyme                                 

Name
lipid II:glycine glycyltransferase;
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase;
femX (gene name);
alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine N6-glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNAGly [RN:R08776]
Reaction(KEGG)
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
The enzyme from Staphylococcus aureus catalyses the transfer of glycine from a charged tRNA to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc (lipid II), attaching it to the N6 of the L-Lys at position 3 of the pentapeptide. This is the first step in the synthesis of the pentaglycine interpeptide bridge that is used in S. aureus for the crosslinking of different glycan strands to each other. Four additional Gly residues are subsequently attached by EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase) and EC 2.3.2.18 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase).
History
EC 2.3.2.16 created 2010
Pathway
Peptidoglycan biosynthesis
Metabolic pathways
Orthology
K11693  
peptidoglycan pentaglycine glycine transferase (the first glycine)
Genes
SAU: 
SA2057(fmhB)
SAV: 
SAV2262(fmhB)
SAW: 
SAHV_2246(fmhB)
SAH: 
SAJ: 
SAM: 
MW2180(fmhB)
SAS: 
SAR: 
SAR2346(fmhB)
SAC: 
SACOL2253(femX)
SAX: 
SAA: 
SAO: 
SAE: 
NWMN_2163(fmhB)
SAD: 
SAAV_2326(femX)
SUU: 
SUV: 
SUH: 
SUE: 
SUJ: 
SUK: 
SUC: 
SUT: 
SUQ: 
SUZ: 
MS7_2282(femX)
SUD: 
SUX: 
SUW: 
SUG: 
SUF: 
SAUA: 
SAUE: 
SAUN: 
SAUS: 
SA40_2008(fmhB)
SAUU: 
SAUZ: 
SAB: 
SAB2134c(fmhB)
SUY: 
SAUB: 
C248_2299(fmhB)
SAUM: 
SAUC: 
SAUR: 
SAUI: 
SAUT: 
SAUJ: 
SAUK: 
SAUQ: 
SAUV: 
SAUW: 
SAUX: 
SAUY: 
SEP: 
SER: 
SERP1844(femX)
SHA: 
SH0790(fmtB)
SSP: 
SCA: 
Sca_1750(fmhB)
SLG: 
SLN: 
SSD: 
SDT: 
SPSE_0559(femX)
SWA: 
SPAS: 
STP1_0738(femX)
SXY: 
MCL: 
ESI: 
EAT: 
EAN: 
EXM: 
PLV: 
AUR: 
ASM: 
ASO: 
ASB: 
FMA: 
CLO: 
 » show all
Taxonomy
Reference
1  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol. Microbiol. 53 (2004) 675-85.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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