KEGG   ENZYME: 2.3.2.18Help
Entry
EC 2.3.2.18                 Enzyme                                 

Name
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase;
femB (gene name);
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNAGly [RN:R08778]
Reaction(KEGG)
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
This Staphylococcus aureus enzyme catalyses the successive transfer of two Gly moieties from charged tRNAs to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc, attaching them to the three Gly molecules that were previously attached to the N6 of the L-Lys at position 3 of the pentapeptide by EC 2.3.2.16 (lipid II:glycine glycyltransferase) and EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase). This is the last step in the synthesis of the pentaglycine interpeptide bridge that is used in this organism for the crosslinking of different glycan strands to each other.
History
EC 2.3.2.18 created 2010
Pathway
Peptidoglycan biosynthesis
Metabolic pathways
Orthology
K11695  
peptidoglycan pentaglycine glycine transferase (the fourth and fifth glycine)
Genes
SAU: 
SA1207(femB)
SAV: 
SAV1375(femB)
SAW: 
SAHV_1363(femB)
SAH: 
SAJ: 
SAM: 
MW1262(femB)
SAS: 
SAR: 
SAR1388(femB)
SAC: 
SAX: 
SAA: 
SAO: 
SAE: 
NWMN_1287(femB)
SAD: 
SUU: 
SUV: 
SUH: 
SUE: 
SUJ: 
SUK: 
SUC: 
SUT: 
SUQ: 
SUZ: 
MS7_1332(femB)
SUD: 
SUX: 
SUW: 
SUG: 
SUF: 
SAUA: 
SAUE: 
SAUN: 
SAUS: 
SA40_1252(femB)
SAUU: 
SAUZ: 
SAB: 
SAB1230(femB)
SUY: 
SAUB: 
C248_1413(femB)
SAUM: 
SAUC: 
SAUR: 
SAUI: 
SAUT: 
SAUJ: 
SAUK: 
SAUQ: 
SAUV: 
SAUW: 
SAUX: 
SAUY: 
SEP: 
SER: 
SERP0947(femB)
SHA: 
SH1531(femB)
SSP: 
SCA: 
Sca_1020(femB)
SLG: 
SLN: 
SSD: 
SDT: 
SPSE_1402(femB)
SWA: 
SPAS: 
STP1_2405(femB)
MCL: 
MCCL_1042(femB)
TPY: 
BSA: 
 » show all
Taxonomy
Reference
1  [PMID:9393725]
  Authors
Ehlert K, Schroder W, Labischinski H
  Title
Specificities of FemA and FemB for different glycine residues: FemB cannot substitute for FemA in staphylococcal peptidoglycan pentaglycine side chain formation.
  Journal
J. Bacteriol. 179 (1997) 7573-6.
  Organism
Staphylococcus aureus
  Sequence
Reference
2  [PMID:14523106]
  Authors
Rohrer S, Berger-Bachi B
  Title
Application of a bacterial two-hybrid system for the analysis of protein-protein  interactions between FemABX family proteins.
  Journal
Microbiology. 149 (2003) 2733-8.
  Organism
Staphylococcus aureus
  Sequence
Reference
3  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol. Microbiol. 53 (2004) 675-85.
  Organism
Staphylococcus aureus
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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