| Entry |
|
|---|
| Name |
2-methylcitrate synthase;
2-methylcitrate oxaloacetate-lyase;
MCS;
methylcitrate synthase;
methylcitrate synthetase |
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| Class |
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
 |
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| Sysname |
propanoyl-CoA:oxaloacetate C-propanoyltransferase
(thioester-hydrolysing, 1-carboxyethyl-forming) |
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| Reaction(IUBMB) |
propanoyl-CoA + H2O + oxaloacetate =
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA [RN:R00931] |
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| Reaction(KEGG) |
R00931
 |
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| Substrate |
propanoyl-CoA [CPD:C00100];
H2O [CPD:C00001];
oxaloacetate [CPD:C00036] |
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| Product |
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate [CPD:C02225];
CoA [CPD:C00010] |
|---|
| Comment |
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and
pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and
oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but
the enzyme has been separated from EC 2.3.3.1 citrate (Si)-synthase.
Oxaloacetate cannot be replaced by glyoxylate, pyruvate or
2-oxoglutarate. |
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| Pathway |
PATH: ec00640 Propanoate metabolism |
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| Orthology |
KO: K01659 2-methylcitrate synthase |
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| Genes |
DDI: DDB_0220637
TET: TTHERM_00537060
ECO: b0333(prpC)
ECJ: JW0324(prpC)
ECE: Z0428(prpC)
ECS: ECs0386
ECF: ECH74115_0404(prpC)
ETW: ECSP_0394(prpC)
ECG: E2348C_0292(prpC)
ECC: c0452(prpC)
ECI: UTI89_C0364(prpC)
ECP: ECP_0408
ECV: APECO1_1656(prpC)
ECW: EcE24377A_0357(prpC)
ECX: EcHS_A0398(prpC)
ECM: EcSMS35_0364(prpC)
ECY: ECSE_0357
ECL: EcolC_3292
ECK: EC55989_0339(prpC)
ECQ: ECED1_0365(prpC)
ECR: ECIAI1_0334(prpC)
ECT: ECIAI39_0347(prpC)
ECZ: ECS88_0344(prpC)
EUM: ECUMN_0376(prpC)
ELF: LF82_1740(prpC)
EFE: EFER_2659(prpC)
STY: STY0401(prpC)
STT: t2495(prpC)
SPT: SPA2354(prpC)
SEK: SSPA2196
SPQ: SPAB_03228
SEI: SPC_0379(prpC)
SEC: SC0410(prpC)
SEH: SeHA_C0463(prpC)
SEE: SNSL254_A0409(prpC)
SEW: SeSA_A0421(prpC)
SEA: SeAg_B0403(prpC)
SED: SeD_A0401(prpC)
SEG: SG0381(prpC)
SET: SEN0352(prpC)
SES: SARI_02556
STM: STM0369(prpC)
PLU: plu3541(prpC)
PAY: PAU_01235(prpC)
CKO: CKO_02829
XCC: XCC1032(prpC)
XCB: XC_3214
XCA: xccb100_3313(prpC)
XCV: XCV1157
XAC: XAC1138(prpC)
XOO: XOO0893(prpC)
XOM: XOO_0818
XOP: PXO_02719
SML: Smlt3609(prpC)
SMT: Smal_3029
VCH: VC1337
VCO: VC0395_A0953(prpC)
VVU: VV1_2731
VVY: VV1530
VPA: VP1647
VHA: VIBHAR_02396
VSP: VS_1344
VEX: VEA_003389
PPR: PBPRA1247 PBPRB0236
PAE: PA0795(prpC)
PAU: PA14_53950(prpC)
PAP: PSPA7_4723(prpC)
PAG: PLES_45471(prpC)
PPU: PP_2335
PPF: Pput_3435
PPG: PputGB1_1936
PPW: PputW619_1776
PST: PSPTO_2288(prpC)
PSB: Psyr_2086
PSP: PSPPH_2057(prpC)
PFL: PFL_1862(prpC)
PFO: Pfl01_1765
PEN: PSEEN1903(prpC)
PMY: Pmen_2081
PSA: PST_2035(prpC)
PAR: Psyc_1111(prpC)
PCR: Pcryo_1310
PRW: PsycPRwf_1198
ACI: ACIAD2756(prpC)
ABM: ABSDF0092(prpC)
ABY: ABAYE3792(prpC)
ABC: ACICU_00100
ABN: AB57_0123
ABB: ABBFA_003438
SON: SO_0344(prpC)
SDN: Sden_1665
SFR: Sfri_1858
SAZ: Sama_3295
SBL: Sbal_4048
SBM: Shew185_4022
SBN: Sbal195_4141
SBP: Sbal223_3946
SLO: Shew_1821
SPC: Sputcn32_3645
SSE: Ssed_2112
SPL: Spea_2321
SHE: Shewmr4_3630
SHM: Shewmr7_0314
SHN: Shewana3_3826
SHW: Sputw3181_3785
SHL: Shal_1962
SWD: Swoo_2498
SWP: swp_2429
ILO: IL1426(prpC)
CPS: CPS_2821
PHA: PSHAa1774(prpC)
PAT: Patl_1420
MAQ: Maqu_1665
AMC: MADE_03371
PIN: Ping_1868
CBU: CBU_0772(prpC)
CBS: COXBURSA331_A1176(prpC)
CBD: CBUD_0820(prpC)
CBG: CbuG_1229(prpC)
CBC: CbuK_0642(prpC)
LPN: lpg1530
LPF: lpl1496(prpC)
LPP: lpp1487(prpC)
LPC: LPC_0951(prpC)
NOC: Noc_2208
AEH: Mlg_2604
HHA: Hhal_1071
TGR: Tgr7_1508
HCH: HCH_02711
CSA: Csal_2420
ABO: ABO_1432(prpC)
MMW: Mmwyl1_0924
AHA: AHA_2266
ASA: ASA_2040(prpC)
NMA: NMA2054(prpC)
NME: NMB0431(pprC)
NMC: NMC1732(prpC)
NMN: NMCC_1714(prpC)
NGO: NGO1525
NGK: NGK_1813
CVI: CV_2056(prpC)
RSO: RSp0121(prpC)
RPI: Rpic_3860
RPF: Rpic12D_3974
REU: Reut_A1809 Reut_A2329
REH: H16_A1906(prpC1) H16_A2636(prpC2)
RME: Rmet_1586
CTI: RALTA_A1600(prpC) RALTA_A2135(prpC2)
BMA: BMAA1869
BMV: BMASAVP1_0878
BML: BMA10229_1165
BMN: BMA10247_A2142
BPS: BPSS0207(prpC)
BPM: BURPS1710b_A1737(prpC)
BPL: BURPS1106A_A0289(prpC)
BPD: BURPS668_A0383(prpC)
BTE: BTH_II2188
BVI: Bcep1808_3679
BUR: Bcep18194_B0142
BCN: Bcen_5345
BCH: Bcen2424_5516
BCM: Bcenmc03_4756
BCJ: BCAM2702(prpC)
BAM: Bamb_4841
BAC: BamMC406_5388
BMU: Bmul_3288
BMJ: BMULJ_05237(prpC)
BXE: Bxe_B2302 Bxe_B2900
BPH: Bphy_4355 Bphy_6707
BPY: Bphyt_6081 Bphyt_6860
BPE: BP2368(prpC)
BPA: BPP3234(prpC)
BBR: BB3686(prpC)
BPT: Bpet1816(prpC)
BAV: BAV1178(prpC)
AJS: Ajs_1635
VEI: Veis_4451
DAC: Daci_5862
DIA: Dtpsy_2079
HAR: HEAR3267(prpC)
MMS: mma_3488(prpC)
NMU: Nmul_A0866
APP: CAP2UW1_0708
HHE: HH0398(prpC)
ABU: Abu_0277(prpC)
GUR: Gura_0072
BBA: Bd2511(cit)
PUB: SAR11_1156(prpC)
RPA: RPA2394(prpC)
RPC: RPC_3511
RPT: Rpal_2647
OCA: OCAR_7075
PDE: Pden_1347
SWI: Swit_4790
ACR: Acry_0621
RRU: Rru_A2319
RCE: RC1_3805(prpC)
DSY: DSY4425
EEL: EUBELI_20659
CGL: NCgl0630(cgl0659) NCgl0666(cgl0696)
CGB: cg0762(prpC2)
CGT: cgR_0779 cgR_0816
CEF: CE0718
CJK: jk1665(prpC)
CUR: cur_0448
CMI: CMM_2371(gltA1)
CMS: CMS_2312
AAU: AAur_1660(gltA) AAur_pTC20150(gltA)
ACH: Achl_1523
RSA: RSal33209_0824
KRH: KRH_15320(prpC)
ACE: Acel_1658
KRA: Krad_0109
RXY: Rxyl_2401
MIN: Minf_0334(gltA)
SYW: SYNW2275(gltA)
PMM: PMM0161(gltA)
PMB: A9601_01791(gltA)
PMC: P9515_01901(gltA)
PMG: P9301_01811(gltA)
PTO: PTO0169
 |
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Reference
Authors
Title
Journal
|
1
Uchiyama, H. and Tabuchi, T.
Properties of methylcitrate synthase from Candida lipolytica.
Agric. Biol. Chem. 40 (1976) 1411-1418. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:9325432]
Textor S, Wendisch VF, De Graaf AA, Muller U, Linder MI, Linder D,
Buckel W.
Propionate oxidation in Escherichia coli: evidence for operation of
a methylcitrate cycle in bacteria.
Arch. Microbiol. 168 (1997) 428-36.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:10482501]
Horswill AR, Escalante-Semerena JC.
Salmonella typhimurium LT2 catabolizes propionate via the
2-methylcitric acid cycle.
J. Bacteriol. 181 (1999) 5615-23.
Salmonella typhimurium [GN:stm] |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:7707698]
van Rooyen JP, Mienie LJ, Erasmus E, De Wet WJ, Ketting D, Duran M,
Wadman SK.
Identification of the stereoisomeric configurations of methylcitric
acid produced by si-citrate synthase and methylcitrate synthase
using capillary gas chromatography-mass spectrometry.
J. Inherit. Metab. Dis. 17 (1994) 738-47.
Candida lipolytica |
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| Other DBs |
ExplorEnz - The Enzyme Database: 2.3.3.5
IUBMB Enzyme Nomenclature: 2.3.3.5
ExPASy - ENZYME nomenclature database: 2.3.3.5
BRENDA, the Enzyme Database: 2.3.3.5
CAS: 57827-78-8 |
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