KEGG   ENZYME: 2.4.1.101Help
Entry
EC 2.4.1.101                Enzyme                                 

Name
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
N-acetylglucosaminyltransferase I;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase;
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I;
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I;
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTI
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Reaction(IUBMB)
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R [RN:R05983]
Reaction(KEGG)
R05983(G)
Show
Substrate
UDP-N-acetyl-D-glucosamine [CPD:C00043];
3-(alpha-D-mannosyl)-beta-D-mannosyl-R [CPD:C04614]
Product
UDP [CPD:C00015];
3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R [CPD:C04880]
Comment
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor. Note that this enzyme acts before N-acetylglucosaminyltransferases II, III, IV, V and VI (click here for diagram).
History
EC 2.4.1.101 created 1983, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984)
Pathway
N-Glycan biosynthesis
Various types of N-glycan biosynthesis
Metabolic pathways
Orthology
K00726  
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
HSA: 
4245(MGAT1)
PTR: 
462340(MGAT1)
PPS: 
100992340(MGAT1)
GGO: 
101154311(MGAT1)
PON: 
100172524(MGAT1)
MCC: 
707375(MGAT1)
MCF: 
102123962(MGAT1)
MMU: 
17308(Mgat1)
RNO: 
81519(Mgat1)
CGE: 
100682529(Mgat1)
HGL: 
TUP: 
102480807(MGAT1)
CFA: 
491674(MGAT1)
AML: 
FCA: 
101082035(MGAT1)
PTG: 
102954826(MGAT1)
BTA: 
BOM: 
102270151(MGAT1)
PHD: 
102322360(MGAT1)
CHX: 
102181772(MGAT1)
SSC: 
CFR: 
102518411(MGAT1)
BACU: 
103001085(MGAT1)
LVE: 
103084987(MGAT1)
ECB: 
102149649(MGAT1)
MYB: 
102262787(MGAT1)
MYD: 
102753141(MGAT1)
PALE: 
102889186(MGAT1)
MDO: 
100019068(MGAT1)
SHR: 
100930793(MGAT1)
OAA: 
100078839(MGAT1)
TGU: 
FAB: 
PHI: 
APLA: 
FPG: 
CLV: 
ASN: 
AMJ: 
PSS: 
CMY: 
ACS: 
PBI: 
XLA: 
379038(mgat1) 379047(MGC52514)
XTR: 
496815(mgat1)
DRE: 
393649(mgat1a) 558524(mgat1b)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
103191271(mgat1)
BFO: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
410105(Mgat1)
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CELE_B0416.6(gly-13) CELE_F48E3.1(gly-12) CELE_M01F1.1(gly-14)
CBR: 
CBG09783(Cbr-gly-14) CBG16168(Cbr-gly-13) CBG16700(Cbr-gly-12)
BMY: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
AT4G38240(CGL1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
102596963(GntI)
OSA: 
DOSA: 
Os02t0832800-01(Os02g0832800)
OBR: 
BDI: 
SBI: 
SORBI_08g003660(SORBIDRAFT_08g003660)
ZMA: 
100274447(si945027c08(99))
SITA: 
ATR: 
s00044p00146360(AMTR_s00044p00146360) s00044p00146510(AMTR_s00044p00146510)
SMO: 
PPP: 
MIS: 
MPP: 
CSL: 
CVR: 
GSL: 
CCP: 
MBR: 
DDI: 
DPP: 
DFA: 
DFA_02421(gnt3)
ACAN: 
PTI: 
TPS: 
EHX: 
GTT: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:6445358]
  Authors
Harpaz N, Schachter H.
  Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
  Journal
J. Biol. Chem. 255 (1980) 4885-93.
  Organism
Bos taurus
Reference
2  [PMID:6452163]
  Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
  Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
  Journal
Biochemistry. 20 (1981) 967-76.
  Organism
Sus scofa
Reference
3  [PMID:6170335]
  Authors
Miyagi T, Tsuiki S.
  Title
Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas.
  Journal
Biochim. Biophys. Acta. 661 (1981) 148-57.
  Organism
Rattus norvegicus
Reference
4  [PMID:6457827]
  Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
  Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
  Journal
J. Biol. Chem. 256 (1981) 11477-82.
  Organism
Sus scofa
Reference
5  [PMID:6450208]
  Authors
Oppenheimer CL, Hill RL.
  Title
Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase.
  Journal
J. Biol. Chem. 256 (1981) 799-804.
  Organism
Oryctolagus cuniculus
Reference
6  [PMID:6366476]
  Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
  Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
  Journal
Methods. Enzymol. 98 (1983) 98-134.
Reference
7  [PMID:6235906]
  Authors
Vella GJ, Paulsen H, Schachter H.
  Title
Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I.
  Journal
Can. J. Biochem. Cell. Biol. 62 (1984) 409-17.
  Organism
Bos taurus
Reference
8  [PMID:11032794]
  Authors
Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM.
  Title
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
  Journal
EMBO. J. 19 (2000) 5269-80.
  Organism
Oryctolagus cuniculus
  Sequence
[up:P27115]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
102576-81-8

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