KEGG   ENZYME: 2.4.1.131Help
Entry
EC 2.4.1.131                Enzyme                                 

Name
GDP-Man:Man3GlcNAc2-PP-dolichol alpha-1,2-mannosyltransferase;
ALG11;
ALG11 mannosyltransferase;
LEW3 (gene name);
At2G40190 (gene name);
gmd3 (gene name);
galactomannan deficiency protein 3;
GDP-mannose:glycolipid 1,2-alpha-D-mannosyltransferase;
glycolipid 2-alpha-mannosyltransferase;
GDP-mannose:glycolipid 2-alpha-D-mannosyltransferase;
GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
GDP-alpha-D-mannose:D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol 2-alpha-D-mannosyltransferase
Reaction(IUBMB)
2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol [RN:R06127 R06128]
Reaction(KEGG)
R06127(G) R06128(G)
Show
Substrate
GDP-alpha-D-mannose [CPD:C00096];
D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Product
GDP [CPD:C00035];
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Comment
The biosynthesis of asparagine-linked glycoproteins (N-linked protein glycosylation) utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. ALG11 mannosyltransferase from Saccharomyces cerevisiae carries out two sequential steps in the formation of the lipid-linked core oligosaccharide, adding two mannose residues in alpha(1->2) linkages to the nascent oligosaccharide.
History
EC 2.4.1.131 created 1984, modified 2011, modified 2012
Pathway
N-Glycan biosynthesis
Various types of N-glycan biosynthesis
Metabolic pathways
Orthology
K03844  
alpha-1,2-mannosyltransferase
Genes
HSA: 
440138(ALG11)
PON: 
100173205(ALG11)
MCC: 
100498659(ALG11)
MMU: 
207958(Alg11)
RNO: 
361174(Alg11)
CGE: 
HGL: 
101712342(Alg11)
TUP: 
102475258(ALG11)
CFA: 
100682632(ALG11)
AML: 
100469498(ALG11)
FCA: 
101091245(ALG11)
PTG: 
102956830(ALG11)
BTA: 
540845(ALG11)
BOM: 
102286251(ALG11)
PHD: 
102336218(ALG11)
CHX: 
102190970(ALG11)
OAS: 
101120844(ALG11)
SSC: 
100152645(ALG11)
CFR: 
102506887(ALG11)
BACU: 
103002538(ALG11)
LVE: 
103089951(ALG11)
ECB: 
100049892(ALG11)
MYB: 
102255926(ALG11)
MYD: 
102762995(ALG11)
PALE: 
102887410(ALG11)
MDO: 
100028147(ALG11)
OAA: 
100082628(ALG11)
GGA: 
418880(ALG11)
MGP: 
100541393(ALG11)
TGU: 
100231100(ALG11)
FAB: 
101818493(ALG11)
PHI: 
102101256(ALG11)
APLA: 
101796223(ALG11)
FPG: 
101910543(ALG11)
FCH: 
102056572(ALG11)
CLV: 
102086030(ALG11)
ASN: 
102370078(ALG11)
AMJ: 
102577351(ALG11)
PSS: 
102453750(ALG11)
CMY: 
102937670(ALG11)
ACS: 
100552965(alg11)
PBI: 
103061317(ALG11)
XLA: 
733388(alg11)
XTR: 
394898(alg11)
DRE: 
407614(alg11)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102363993(ALG11)
CMK: 
103177168(alg11)
BFO: 
CIN: 
SPU: 
582224(alg11)
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
552533(GB13757)
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CBR: 
BMY: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
TAD: 
ATH: 
AT2G40190(LEW3)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os12t0583000-01(Os12g0583000)
OBR: 
BDI: 
SBI: 
SORBI_08g019540(SORBIDRAFT_08g019540)
ZMA: 
SITA: 
ATR: 
s00019p00025160(AMTR_s00019p00025160)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YNL048W(ALG11)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0G03240(NCAS0G03240)
NDI: 
NDAI_0D01260(NDAI0D01260)
TPF: 
TPHA_0D01640(TPHA0D01640)
TBL: 
TBLA_0B05860(TBLA0B05860)
TDL: 
TDEL_0C03950(TDEL0C03950)
KAF: 
KAFR_0H03470(KAFR0H03470)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.10972(ALG11) CaO19.3468(ALG11)
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_858164(AO090001000490)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
SPCC330.08(alg11)
CNE: 
CNB: 
CGI: 
TMS: 
PPL: 
DSQ: 
SHS: 
PCO: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT123007(AGABI1DRAFT_123007)
ABV: 
AGABI2DRAFT202169(AGABI2DRAFT_202169)
CPUT: 
SLA: 
UMA: 
PFP: 
MGL: 
PGR: 
MLR: 
WSE: 
MBR: 
DDI: 
DPP: 
DFA: 
DFA_11738(alg11)
EHI: 
EHI_093950(3.t00023)
EDI: 
ACAN: 
CPV: 
CHO: 
TET: 
PTM: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
TVA: 
 » show all
Taxonomy
Reference
1  [PMID:16878994]
  Authors
O'Reilly MK, Zhang G, Imperiali B
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry. 45 (2006) 9593-603.
  Sequence
[sce:YNL048W]
Reference
2  [PMID:19929855]
  Authors
Absmanner B, Schmeiser V, Kampf M, Lehle L
  Title
Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an alpha1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide.
  Journal
Biochem. J. 426 (2010) 205-17.
  Sequence
[sce:YNL048W]
Reference
3  [PMID:6154707]
  Authors
Schutzbach JS, Springfield JD, Jensen JW.
  Title
The biosynthesis of oligosaccharide-lipids. Formation of an alpha-1,2-mannosyl-mannose linkage.
  Journal
J. Biol. Chem. 255 (1980) 4170-5.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
74506-43-7

DBGET integrated database retrieval system