KEGG   ENZYME: 2.4.1.14Help
Entry
EC 2.4.1.14                 Enzyme                                 

Name
sucrose-phosphate synthase;
UDP-glucose---fructose-phosphate glucosyltransferase;
sucrosephosphate---UDP glucosyltransferase;
UDP-glucose-fructose-phosphate glucosyltransferase;
SPS;
uridine diphosphoglucose-fructose phosphate glucosyltransferase;
sucrose 6-phosphate synthase;
sucrose phosphate synthetase;
sucrose phosphate-uridine diphosphate glucosyltransferase;
sucrose phosphate synthase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Reaction(IUBMB)
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6F-phosphate [RN:R00766 R06073]
Reaction(KEGG)
Substrate
UDP-glucose [CPD:C00029];
D-fructose 6-phosphate [CPD:C00085]
Product
UDP [CPD:C00015];
sucrose 6F-phosphate [CPD:C02591]
Comment
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
History
EC 2.4.1.14 created 1961, modified 2008
Pathway
Starch and sucrose metabolism
Metabolic pathways
Orthology
K00696  
sucrose-phosphate synthase
Genes
ATH: 
AT1G04920(SPS3F) AT4G10120(ATSPS4F) AT5G11110(SPS2F) AT5G20280(SPS1F)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os02t0184400-01(Os02g0184400) Os08t0301500-01(Os08g0301500)
OBR: 
BDI: 
SBI: 
SORBI_03g043900(SORBIDRAFT_03g043900) SORBI_04g005720(SORBIDRAFT_04g005720) SORBI_05g007310(SORBIDRAFT_05g007310) SORBI_09g028570(SORBIDRAFT_09g028570) SORBI_10g025240(SORBIDRAFT_10g025240)
ZMA: 
SITA: 
ATR: 
s00033p00204130(AMTR_s00033p00204130) s00061p00145220(AMTR_s00061p00145220) s00099p00104230(AMTR_s00099p00104230)
SMO: 
PPP: 
CRE: 
VCN: 
CSL: 
CVR: 
VSP: 
VSA: 
MMT: 
MAH: 
TCX: 
MEJ: 
MEC: 
NOC: 
NHL: 
NWA: 
ALV: 
TVI: 
TMB: 
HHC: 
TGR: 
TKM: 
TNI: 
TVNIR_0279(mfpsA_[H])
HNA: 
AFE: 
AFR: 
ACU: 
AFI: 
NEU: 
NE1213(sps)
NET: 
NIT: 
NII: 
NMU: 
MFA: 
MEI: 
MEP: 
DBA: 
DAK: 
DSF: 
DOL: 
DAT: 
MEX: 
MEA: 
MDI: 
MCH: 
MPO: 
MGM: 
BSUB: 
HOR: 
HAS: 
HPK: 
HHL: 
RBA: 
RB5197(sps)
PLM: 
PBS: 
SYN: 
sll0045(sps)
SYZ: 
SYY: 
SYT: 
SYS: 
SYQ: 
SYW: 
SYC: 
SYF: 
SYD: 
SYE: 
SYG: 
SYR: 
SYX: 
SYP: 
SYNE: 
TEL: 
tlr0582(sps)
THN: 
CGC: 
OAC: 
MIC: 
NPU: 
NOS: 
NOP: 
ANB: 
ACY: 
CSG: 
CALT: 
PMA: 
Pro_1880(rfaG)
PMM: 
PMM1711(sps)
PMT: 
PMT2268(sps)
PMN: 
PMI: 
PMB: 
PMC: 
PMF: 
PMG: 
PMH: 
PMJ: 
PME: 
LBY: 
RSI: 
FJO: 
MRO: 
TME: 
TAF: 
FNO: 
FPE: 
PMO: 
KOL: 
MPG: 
DIN: 
DAP: 
 » show all
Taxonomy
Reference
1  [PMID:13769376]
  Authors
MENDICINO J.
  Title
Sucrose phosphate synthesis in wheat germ and green leaves.
  Journal
J. Biol. Chem. 235 (1960) 3347-52.
  Organism
Triticum aestivum, Spinacia oleracea
Reference
2  [PMID:9852031]
  Authors
Curatti L, Folco E, Desplats P, Abratti G, Limones V, Herrera-Estrella L, Salerno G
  Title
Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
  Journal
J. Bacteriol. 180 (1998) 6776-9.
  Organism
Synechocystis sp.
Reference
3  [PMID:15012296]
  Authors
Huber SC, Huber JL
  Title
ROLE AND REGULATION OF SUCROSE-PHOSPHATE SYNTHASE IN HIGHER PLANTS.
  Journal
Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 47 (1996) 431-444.
  Organism
Zea mays, Spinacia oleracea
Reference
4  [PMID:12062401]
  Authors
Cumino A, Curatti L, Giarrocco L, Salerno GL
  Title
Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.
  Journal
FEBS. Lett. 517 (2002) 19-23.
  Organism
Anabaena sp.
Reference
5  [PMID:18424616]
  Authors
Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J
  Title
The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.
  Journal
Plant. Cell. 20 (2008) 1059-72.
  Organism
Halothermothrix orenii
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9030-06-2

DBGET integrated database retrieval system