KEGG   ENZYME: 2.4.1.186Help
Entry
EC 2.4.1.186                Enzyme                                 

Name
glycogenin glucosyltransferase;
glycogenin;
priming glucosyltransferase;
UDP-glucose:glycogenin glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucose:glycogenin alpha-D-glucosyltransferase
Reaction(IUBMB)
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin [RN:R03681]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
glycogenin [CPD:C01702]
Product
UDP [CPD:C00015];
alpha-D-glucosylglycogenin [CPD:C02687]
Comment
The first reaction of this enzyme is to catalyse its own glucosylation, normally at Tyr-194 of the protein if this group is free. When Tyr-194 is replaced by Thr or Phe, the enzyme's Mn2+-dependent self-glucosylation activity is lost but its intermolecular transglucosylation ability remains [7]. It continues to glucosylate an existing glucosyl group until a length of about 5--13 residues has been formed. Further lengthening of the glycogen chain is then carried out by EC 2.4.1.11, glycogen (starch) synthase. The enzyme is not highly specific for the donor, using UDP-xylose in addition to UDP-glucose (although not glucosylating or xylosylating a xylosyl group so added). It can also use CDP-glucose and TDP-glucose, but not ADP-glucose or GDP-glucose. Similarly it is not highly specific for the acceptor, using water (i.e. hydrolysing UDP-glucose) among others. Various forms of the enzyme exist, and different forms predominate in different organs. Thus primate liver contains glycogenin-2, of molecular mass 66 kDa, whereas the more widespread form is glycogenin-1, with a molecular mass of 38 kDa.
History
EC 2.4.1.186 created 1992 (EC 2.4.1.112 created 1984, incorporated 2007)
Orthology
K00750  
glycogenin glucosyltransferase
Genes
HSA: 
2992(GYG1) 8908(GYG2)
PTR: 
460765(GYG1) 465469(GYG2)
PPS: 
100977408(GYG1) 100995852(GYG2)
GGO: 
101130219(GYG1) 101153384(GYG2)
PON: 
100431369(GYG2) 100434629(GYG1)
NLE: 
100579312(GYG1) 100598771(GYG2)
MCC: 
712495(GYG1) 720568(GYG2)
MCF: 
CJC: 
100405072(GYG1) 100407906(GYG2)
MMU: 
27357(Gyg)
RNO: 
81675(Gyg1)
CGE: 
100751671(Gyg1)
NGI: 
103745990(Gyg1)
HGL: 
101709254(Gyg2) 101716513(Gyg1)
OCU: 
100009058(GYG1)
TUP: 
102473120(GYG1) 102475805(GYG2)
CFA: 
491717(GYG2) 611614(GYG1)
AML: 
UMR: 
103666302(GYG2) 103681483(GYG1)
FCA: 
101080946(GYG2) 101082176(GYG1)
PTG: 
102959321(GYG2) 102968386(GYG1)
BTA: 
280812(GYG1) 505258(GYG2)
BOM: 
PHD: 
102332563(GYG2) 102338836(GYG1)
CHX: 
102175944(GYG2) 102184201(GYG1)
OAS: 
101103289(GYG1) 101115765(GYG2)
SSC: 
100620160(GYG1)
CFR: 
102509684(GYG1) 102514856(GYG2)
BACU: 
103001146(GYG1) 103003273(GYG2)
LVE: 
103082327(GYG2) 103089765(GYG1)
ECB: 
100058811(GYG1) 100064536(GYG2)
MYB: 
102241317(GYG2) 102244006(GYG1)
MYD: 
102758721(GYG2) 102770013(GYG1)
PALE: 
102881568(GYG1) 102897839(GYG2)
MDO: 
100010174(GYG2) 100012580(GYG1)
SHR: 
100926747(GYG1) 100931311(GYG2)
OAA: 
100075592(GYG2)
GGA: 
418660(GYG2) 425050(GYG1)
MGP: 
APLA: 
101789597(GYG1) 101797402(GYG2)
TGU: 
FAB: 
101817442(GYG2) 101821265(GYG1)
PHI: 
FPG: 
101918206(GYG2) 101922940(GYG1)
FCH: 
102045779(GYG1) 102056432(GYG2)
CLV: 
102093487(GYG2) 102093817(GYG1)
ASN: 
102371930(GYG1)
AMJ: 
102570451(GYG1)
PSS: 
102455672(GYG2) 102456769(GYG1)
CMY: 
102942072(GYG1) 102942316(GYG2)
ACS: 
100557488(gyg2) 100562212(gyg1)
PBI: 
XLA: 
100126641(gyg2) 446485(gyg1) 779133(gyg)
XTR: 
394883(gyg1) 496877(gyg2)
DRE: 
406831(gyg1a) 415152(gyg1b) 561803(gyg2)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
103184449(gyg1)
BFO: 
CIN: 
SPU: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
CQU: 
AME: 
552421(GB16294)
NVI: 
TCA: 
API: 
PHU: 
ISC: 
CEL: 
CBR: 
CBG13253 CBG14232(Cbr-uvt-5)
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
GMX: 
PVU: 
MTR: 
CAM: 
PPER: 
PMUM: 
MDM: 
CMO: 
RCU: 
POP: 
SOT: 
DOSA: 
Os05t0426400-01(Os05g0426400)
SBI: 
SORBI_03g040240(SORBIDRAFT_03g040240)
SITA: 
ATR: 
s00025p00246110(AMTR_s00025p00246110)
SMO: 
PPP: 
BPG: 
CVR: 
CCP: 
ERC: 
KLA: 
ZRO: 
NCS: 
NCAS_0B08120(NCAS0B08120)
NDI: 
NDAI_0B05430(NDAI0B05430)
TPF: 
TPHA_0A02970(TPHA0A02970)
TBL: 
TBLA_0D05740(TBLA0D05740)
TDL: 
TDEL_0H00940(TDEL0H00940)
KAF: 
KAFR_0G00660(KAFR0G00660)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.7434(GLG22)
CTP: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
CTHR: 
TMN: 
TRE: 
MAW: 
CMT: 
CIM: 
URE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
NPA: 
DSQ: 
PCO: 
SHS: 
ADL: 
FME: 
LBC: 
MLR: 
MBR: 
DFA: 
ACAN: 
TGO: 
TPS: 
PIF: 
NGD: 
EHX: 
GTT: 
NGR: 
TVA: 
MBJ: 
 » show all
Taxonomy
Reference
1  [PMID:809265]
  Authors
Krisman CR, Barengo R.
  Title
A precursor of glycogen biosynthesis: alpha-1,4-glucan-protein.
  Journal
Eur. J. Biochem. 52 (1975) 117-23.
Reference
2  [PMID:3121316]
  Authors
Pitcher J, Smythe C, Campbell DG, Cohen P.
  Title
Identification of the 38-kDa subunit of rabbit skeletal muscle glycogen synthase as glycogenin.
  Journal
Eur. J. Biochem. 169 (1987) 497-502.
Reference
3  [PMID:2970965]
  Authors
Pitcher J, Smythe C, Cohen P.
  Title
Glycogenin is the priming glucosyltransferase required for the initiation of glycogen biogenesis in rabbit skeletal muscle.
  Journal
Eur. J. Biochem. 176 (1988) 391-5.
Reference
4
  Authors
Kennedy, L.D., Kirkman, B.R., Lomako, J., Rodriguez, I.R. and Whelan, W.J.
  Title
The biogenesis of rabbit-muscle glycogen.
  Journal
In: Berman, M.C. and Opie, L.A. (Eds.), Membranes and Muscle, ICSU Press/IRL Press, Oxford, 1985, p. 65-84.
Reference
5  [PMID:4062948]
  Authors
Rodriguez IR, Whelan WJ.
  Title
A novel glycosyl-amino acid linkage: rabbit-muscle glycogen is covalently linked to a protein via tyrosine.
  Journal
Biochem. Biophys. Res. Commun. 132 (1985) 829-36.
Reference
6  [PMID:2973423]
  Authors
Lomako J, Lomako WM, Whelan WJ.
  Title
A self-glucosylating protein is the primer for rabbit muscle glycogen biosynthesis.
  Journal
FASEB. J. 2 (1988) 3097-103.
Reference
7  [PMID:7797519]
  Authors
Alonso MD, Lomako J, Lomako WM, Whelan WJ.
  Title
Catalytic activities of glycogenin additional to autocatalytic self-glucosylation.
  Journal
J. Biol. Chem. 270 (1995) 15315-9.
Reference
8  [PMID:7672505]
  Authors
Alonso MD, Lomako J, Lomako WM, Whelan WJ.
  Title
A new look at the biogenesis of glycogen.
  Journal
FASEB. J. 9 (1995) 1126-37.
Reference
9  [PMID:9857012]
  Authors
Mu J, Roach PJ.
  Title
Characterization of human glycogenin-2, a self-glucosylating initiator of liver glycogen metabolism.
  Journal
J. Biol. Chem. 273 (1998) 34850-6.
  Sequence
[hsa:8908]
Reference
10 [PMID:12051921]
  Authors
Gibbons BJ, Roach PJ, Hurley TD.
  Title
Crystal structure of the autocatalytic initiator of glycogen biosynthesis, glycogenin.
  Journal
J. Mol. Biol. 319 (2002) 463-77.
  Sequence
[up:P13280]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
117590-73-5

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