KEGG   ENZYME: 2.4.1.198Help
Entry
EC 2.4.1.198                Enzyme                                 
Name phosphatidylinositol N-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:phosphatidylinositol
N-acetyl-D-glucosaminyltransferase;
uridine diphosphoacetylglucosamine
alpha1,6-acetyl-D-glucosaminyltransferase
Class Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname UDP-N-acetyl-D-glucosamine:1-phosphatidyl-1D-myo-inositol
6-(N-acetyl-alpha-D-glucosaminyl)transferase
Reaction(IUBMB) UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP +
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
[RN:R02654 R05916]
Reaction(KEGG) R02654 R05916(G)
Show
Substrate UDP-N-acetyl-D-glucosamine [CPD:C00043];
1-phosphatidyl-1D-myo-inositol [CPD:C01194]
Product UDP [CPD:C00015];
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
[CPD:C01288]
Comment Involved in the first step of glycosylphosphatidylinositol (GPI)
anchor formation in all eukaryotes. In mammalian cells, the enzyme
is composed of at least five subunits (PIG-A, PIG-H, PIG-C, GPI1 and
PIG-P). PIG-A subunit is the catalytic subunit. In some species, the
long-chain acyl groups of the phosphatidyl group are partly replaced
by long-chain alkyl or alk-1-enyl groups.
Pathway PATH: ec00563  Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
PATH: ec01100  Metabolic pathways
Orthology KO: K03857  phosphatidylinositol glycan, class A
Genes HSA: 5277(PIGA)
PTR: 465507(PIGA)
MCC: 712514
MMU: 18700(Piga)
RNO: 363464(Piga)
CFA: 491748(PIGA)
SSC: 100156880
ECB: 100055827(PIGA)
MDO: 100031813
OAA: 100085170
GGA: 418624(PIGA)
DRE: 791759(piga)
BFO: BRAFLDRAFT_243162
CIN: 100178746
SPU: 584339
DME: Dmel_CG6401
DPO: Dpse_GA19564
DAN: Dana_GF13228
DER: Dere_GG21024
DPE: Dper_GL17279
DSE: Dsec_GM19954
DSI: Dsim_GD25445
DYA: Dyak_GE13967
DGR: Dgri_GH22654
DMO: Dmoj_GI21166
AGA: AgaP_AGAP011274
AAG: AaeL_AAEL005468
CQU: CpipJ_CPIJ005143
AME: 413643
API: 100161768
ISC: IscW_ISCW020444
CEL: D2085.6
CBR: CBG00513
BMY: Bm1_42330
SMM: Smp_046880.1
NVE: NEMVE_v1g158845
HMG: 100203340
TAD: TRIADDRAFT_23147
ATH: AT3G45100(SETH2)
POP: POPTR_254214
RCU: RCOM_0545690
VVI: 100256927
SBI: SORBI_02g008800(SORBIDRAFT_02g008800)
PPP: PHYPADRAFT_214829
CRE: CHLREDRAFT_139676(GTR18)
OLU: OSTLU_49124
SCE: YPL175W(SPT14)
AGO: AGOS_AAL108C
KLA: KLLA0C09713g
LTH: KLTH0D11880g
DHA: DEHA0F16126g
PIC: PICST_50490(SPT14)
PPA: PAS_chr2-1_0303
VPO: Kpol_1064p18
LEL: LELG_03358
ZRO: ZYRO0F11836g
CAL: CaO19.487(SPT14)
CTP: CTRG_00367
CDU: CD36_29330
CGR: CAGL0F03597g
YLI: YALI0A20482g
SPO: SPBC3D6.07(gpi3)
NCR: NCU09757
PAN: PODANSg6855
MGR: MGG_03933
FGR: FG00960.1
ANI: AN0636.2
AFM: AFUA_1G16950
AOR: AO090005000980
ANG: An01g09910
AFV: AFLA_082330
PCS: Pc21g23560
NFI: NFIA_008430
CIM: CIMG_02994
URE: UREG_02953
SSL: SS1G_10076
BFU: BC1G_12524
CNE: CND02330
CNB: CNBD4030
LBC: LACBIDRAFT_191321
MPR: MPER_11396
UMA: UM05234.1
MGL: MGL_1728
ECU: ECU09_1210
MBR: MONBRDRAFT_33045
DDI: DDB_0231698(pigA)
EHI: EHI_105870
EDI: EDI_111430
PFA: PF10_0316
PFD: PFDG_02018
PFH: PFHG_01314
PYO: PY03143
PCB: PC000590.00.0
PBE: PB106808.00.0
PKN: PKH_061680
PVX: PVX_111105
TAN: TA10130
TPV: TP04_0759
BBO: BBOV_III008560
CPV: cgd4_2100
CHO: Chro.40236
TET: TTHERM_00449060
PTM: GSPATT00001670001 GSPATT00028092001
TBR: Tb927.2.1780
TCR: 509215.16
LMA: LmjF33.1670
LIF: LinJ33.1770
LBZ: LbrM33_V2.1940
GLA: GL50803_113610
PTI: PHATRDRAFT_54068
TPS: THAPSDRAFT_181
Taxonomy
Reference
  Authors
  Title


  Journal
  Organism
 1  [PMID:2525555]
Doering TL, Masterson WJ, Englund PT, Hart GW.
Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of
the trypanosome variant surface glycoprotein. Origin of the
non-acetylated glucosamine.
J. Biol. Chem. 264 (1989) 11168-73.
Trypanosoma brucei [GN:tbr]
Reference
  Authors

  Title

  Journal
  Organism

 2  [PMID:9463366]
Watanabe R, Inoue N, Westfall B, Taron CH, Orlean P, Takeda J,
Kinoshita T.
The first step of glycosylphosphatidylinositol biosynthesis is
mediated by a complex of PIG-A, PIG-H, PIG-C and GPI1.
EMBO. J. 17 (1998) 877-85.
Homo sapiens [GN:hsa], Saccharomyces cerevisiae [GN:sce], Plasmodium
falciparum [GN:pfa], Trypanosoma brucei [GN:tbr]
Reference
  Authors

  Title

  Journal
  Organism
 3  [PMID:10944123]
Watanabe R, Murakami Y, Marmor MD, Inoue N, Maeda Y, Hino J, Kangawa
K, Julius M, Kinoshita T.
Initial enzyme for glycosylphosphatidylinositol biosynthesis
requires PIG-P and is regulated by DPM2.
EMBO. J. 19 (2000) 4402-11.
Plasmodium falciparum [GN:pfa], Trypanosoma brucei [GN:tbr]
Other DBs ExplorEnz - The Enzyme Database: 2.4.1.198
IUBMB Enzyme Nomenclature: 2.4.1.198
ExPASy - ENZYME nomenclature database: 2.4.1.198
BRENDA, the Enzyme Database: 2.4.1.198
CAS: 144388-35-2
KEGG   ENZYME: 3.5.1.89Help
Entry
EC 3.5.1.89                 Enzyme                                 
Name N-acetylglucosaminylphosphatidylinositol deacetylase;
N-acetyl-D-glucosaminylphosphatidylinositol acetylhydrolase;
N-acetylglucosaminylphosphatidylinositol de-N-acetylase;
GlcNAc-PI de-N-acetylase;
GlcNAc-PI deacetylase;
acetylglucosaminylphosphatidylinositol deacetylase
Class Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
acetylhydrolase
Reaction(IUBMB) 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol +
H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol +
acetate [RN:R03482 R05917]
Reaction(KEGG) R03482 R05917(G)
Show
Substrate 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
[CPD:C01288];
H2O [CPD:C00001]
Product 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
[CPD:C04248];
acetate [CPD:C00033]
Comment Involved in the second step of glycosylphosphatidylinositol (GPI)
anchor formation in all eukaryotes. The enzyme appears to be
composed of a single subunit (PIG-L in mammalian cells and GPI12 in
yeast). In some species, the long-chain sn-1-acyl group of the
phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl
group.
Pathway PATH: ec00563  Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
PATH: ec01100  Metabolic pathways
Orthology KO: K03434  N-acetylglucosaminylphosphatidylinositol deacetylase
Genes HSA: 9487(PIGL)
PTR: 468371(PIGL)
MCC: 696605(PIGL)
MMU: 327942(Pigl)
RNO: 192263(Pigl)
CFA: 479514(PIGL)
BTA: 507080(PIGL)
ECB: 100073146
TGU: 100218498
BFO: BRAFLDRAFT_246699
CIN: 100184871
DME: Dmel_CG4433
DPO: Dpse_GA18180
DAN: Dana_GF17341
DER: Dere_GG15675
DPE: Dper_GL12522
DSE: Dsec_GM17700
DSI: Dsim_GD20086
DYA: Dyak_GE25096
DGR: Dgri_GH23249
DMO: Dmoj_GI10317
AGA: AgaP_AGAP001955
AAG: AaeL_AAEL004143
CQU: CpipJ_CPIJ011635
AME: 724648
NVI: 100122783
TCA: 660665
ISC: IscW_ISCW019246
BMY: Bm1_48430
SMM: Smp_035080
HMG: 100200643
TAD: TRIADDRAFT_57271
POP: POPTR_891844
RCU: RCOM_1185650
VVI: 100249862
SBI: SORBI_06g020130(SORBIDRAFT_06g020130)
     SORBI_06g033040(SORBIDRAFT_06g033040)
ZMA: 100283695
PPP: PHYPADRAFT_145059
OLU: OSTLU_34729
SCE: YMR281W(GPI12)
AGO: AGOS_ABL120W
KLA: KLLA0D01430g
LTH: KLTH0C03740g
DHA: DEHA0F04664g
PIC: PICST_30247(GPI12)
PPA: PAS_chr1-1_0044
VPO: Kpol_1068p4
LEL: LELG_00670
ZRO: ZYRO0G14300g
CTP: CTRG_03326
CDU: CD36_09720
CGR: CAGL0M03047g
YLI: YALI0E21934g
SPO: SPAPB2B4.01c(gpi12)
NCR: NCU09510
PAN: PODANSg2422 PODANSg4679
MGR: MGG_05020
ANI: AN0049.2
AFM: AFUA_5G12550
AOR: AO090120000381
ANG: An14g06640
AFV: AFLA_093620
PCS: Pc21g04290
NFI: NFIA_074660
CIM: CIMG_09320
URE: UREG_04986
SSL: SS1G_11606
BFU: BC1G_10984
CNE: CNC00580
CNB: CNBC6650
LBC: LACBIDRAFT_297620
UMA: UM00302.1
MGL: MGL_0955
DDI: DDB_0235227(pigL)
EHI: EHI_015270
EDI: EDI_239010
PFD: PFDG_04448
PFH: PFHG_00599
PCB: PC001117.02.0
PBE: PB001100.03.0
TAN: TA10880
TPV: TP04_0615
BBO: BBOV_III007080
TET: TTHERM_00471420
PTM: GSPATT00009494001 GSPATT00019956001
TBR: Tb11.01.3900
TCR: 504005.20 511481.40
LMA: LmjF09.0040(GPI12)
LIF: LinJ09.0060
LBZ: LbrM09_V2.0060
PTI: PHATRDRAFT_34610
TPS: THAPSDRAFT_8003
FAL: FRAAL3175
Taxonomy
Reference
  Authors
  Title


  Journal
  Organism
 1  [PMID:2525555]
Doering TL, Masterson WJ, Englund PT, Hart GW.
Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of
the trypanosome variant surface glycoprotein. Origin of the
non-acetylated glucosamine.
J. Biol. Chem. 264 (1989) 11168-73.
Trypanosoma brucei [GN:tbr]
Reference
  Authors

  Title

  Journal
  Organism
 2  [PMID:9188481]
Nakamura N, Inoue N, Watanabe R, Takahashi M, Takeda J, Stevens VL,
Kinoshita T.
Expression cloning of PIG-L, a candidate
N-acetylglucosaminyl-phosphatidylinositol deacetylase.
J. Biol. Chem. 272 (1997) 15834-40.
Saccharomyces cerevisiae [GN:sce], Rattus norvegicus [GN:rno]
Reference
  Authors
  Title


  Journal
  Organism
 3  [PMID:10085243]
Watanabe R, Ohishi K, Maeda Y, Nakamura N, Kinoshita T.
Mammalian PIG-L and its yeast homologue Gpi12p are
N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential
in glycosylphosphatidylinositol biosynthesis.
Biochem. J. 339 ( Pt 1) (1999) 185-92.
Saccharomyces cerevisiae [GN:sce], Rattus norvegicus [GN:rno]
Reference
  Authors

  Title

  Journal
  Organism
 4  [PMID:11432820]
Smith TK, Crossman A, Borissow CN, Paterson MJ, Dix A, Brimacombe
JS, Ferguson MA.
Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and
synthesis of parasite-specific suicide substrate inhibitors.
EMBO. J. 20 (2001) 3322-32.
Homo sapiens [GN:hsa], Trypanosoma brucei [GN:tbr]
Other DBs ExplorEnz - The Enzyme Database: 3.5.1.89
IUBMB Enzyme Nomenclature: 3.5.1.89
ExPASy - ENZYME nomenclature database: 3.5.1.89
BRENDA, the Enzyme Database: 3.5.1.89
CAS: 122191-30-4
DBGET integrated database retrieval system